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Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis

  • Authors:
    • Jia Wang
    • Hao Li
    • Bo Li
    • Qiulin Gong
    • Xinmin Chen
    • Qi Wang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Chengdu Fifth People's Hospital, Chengdu, Sichuan 611130, P.R. China, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 567-572
    |
    Published online on: May 24, 2016
       https://doi.org/10.3892/etm.2016.3386
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Abstract

Diabetic periodontitis (DP), which has been shown to cause alveolar bone loss, is among the most common complications associated with diabetes. The precise mechanisms underlying alveolar bone loss in patients with DP remain unclear. Therefore, the present study established a co‑culture system of bone marrow stem cells (BMSCs) and macrophages, in order to investigate the potential mechanisms underlying DP‑associated alveolar bone loss in vitro. In addition, Porphyromonas gingivalis (PG) periodontal infection and high glucose levels were used to induce DP in mice. The present study evaluated the protein expression levels of various chemokines and the migration of BMSCs and macrophages. The protein expression levels of extracellular signal‑regulated kinase 1 and 2, c‑Jun N‑terminal kinase and p38 mitogen-activated protein kinase (MAPK) were significantly increased in the BMSCs exposed to high glucose and PG, which may have been due to the activation of MAPK. In addition, DP induction in mice was associated with the release of chemokine (C‑C motif) ligand 2 (CCL2) from BMSCs and the secretion of chemokine (C‑C Motif) receptor 2 (CCR2) and tumor necrosis factor‑α from macrophages, which was associated in turn with enhanced adhesion and chemotaxis of macrophages. The results of the present study suggested that DP led to the upregulation of CCL2 in the periodontal tissues and enhanced macrophage infiltration via the CCL2/CCR2 axis, which in turn promoted alveolar bone loss.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Li H, Li B, Gong Q, Chen X and Wang Q: Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis. Exp Ther Med 12: 567-572, 2016.
APA
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., & Wang, Q. (2016). Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis. Experimental and Therapeutic Medicine, 12, 567-572. https://doi.org/10.3892/etm.2016.3386
MLA
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., Wang, Q."Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis". Experimental and Therapeutic Medicine 12.2 (2016): 567-572.
Chicago
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., Wang, Q."Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis". Experimental and Therapeutic Medicine 12, no. 2 (2016): 567-572. https://doi.org/10.3892/etm.2016.3386
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Li H, Li B, Gong Q, Chen X and Wang Q: Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis. Exp Ther Med 12: 567-572, 2016.
APA
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., & Wang, Q. (2016). Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis. Experimental and Therapeutic Medicine, 12, 567-572. https://doi.org/10.3892/etm.2016.3386
MLA
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., Wang, Q."Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis". Experimental and Therapeutic Medicine 12.2 (2016): 567-572.
Chicago
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., Wang, Q."Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis". Experimental and Therapeutic Medicine 12, no. 2 (2016): 567-572. https://doi.org/10.3892/etm.2016.3386
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