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Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis

  • Authors:
    • Jia Wang
    • Hao Li
    • Bo Li
    • Qiulin Gong
    • Xinmin Chen
    • Qi Wang

  • View Affiliations

  • Published online on: May 24, 2016     https://doi.org/10.3892/etm.2016.3386
  • Pages: 567-572

  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic periodontitis (DP), which has been shown to cause alveolar bone loss, is among the most common complications associated with diabetes. The precise mechanisms underlying alveolar bone loss in patients with DP remain unclear. Therefore, the present study established a co‑culture system of bone marrow stem cells (BMSCs) and macrophages, in order to investigate the potential mechanisms underlying DP‑associated alveolar bone loss in vitro. In addition, Porphyromonas gingivalis (PG) periodontal infection and high glucose levels were used to induce DP in mice. The present study evaluated the protein expression levels of various chemokines and the migration of BMSCs and macrophages. The protein expression levels of extracellular signal‑regulated kinase 1 and 2, c‑Jun N‑terminal kinase and p38 mitogen-activated protein kinase (MAPK) were significantly increased in the BMSCs exposed to high glucose and PG, which may have been due to the activation of MAPK. In addition, DP induction in mice was associated with the release of chemokine (C‑C motif) ligand 2 (CCL2) from BMSCs and the secretion of chemokine (C‑C Motif) receptor 2 (CCR2) and tumor necrosis factor‑α from macrophages, which was associated in turn with enhanced adhesion and chemotaxis of macrophages. The results of the present study suggested that DP led to the upregulation of CCL2 in the periodontal tissues and enhanced macrophage infiltration via the CCL2/CCR2 axis, which in turn promoted alveolar bone loss.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang J, Li H, Li B, Gong Q, Chen X and Wang Q: Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis. Exp Ther Med 12: 567-572, 2016
APA
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., & Wang, Q. (2016). Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis. Experimental and Therapeutic Medicine, 12, 567-572. https://doi.org/10.3892/etm.2016.3386
MLA
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., Wang, Q."Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis". Experimental and Therapeutic Medicine 12.2 (2016): 567-572.
Chicago
Wang, J., Li, H., Li, B., Gong, Q., Chen, X., Wang, Q."Co-culture of bone marrow stem cells and macrophages indicates intermediate mechanism between local inflammation and innate immune system in diabetic periodontitis". Experimental and Therapeutic Medicine 12, no. 2 (2016): 567-572. https://doi.org/10.3892/etm.2016.3386