Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation

  • Authors:
    • Feijia Ding
    • Fan Li
    • Yunhong Li
    • Xiaolin Hou
    • Yi Ma
    • Nan Zhang
    • Jiao Ma
    • Rui Zhang
    • Bing Lang
    • Hongyan Wang
    • Yin Wang
  • View Affiliations / Copyright

    Affiliations: Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Ningxia 750004, P.R. China, Department of Neurology, The General Hospital of Ningxia Medical University, Ningxia 750004, P.R. China, Department of ICU, The First People's Hospital of Yinchuan, Ningxia 750004, P.R. China
  • Pages: 823-828
    |
    Published online on: June 1, 2016
       https://doi.org/10.3892/etm.2016.3413
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Curcumin has anti-inflammatory and antioxidant properties and has been widely used to treat or prevent neurodegenerative diseases. However, the mechanisms underlying the neuroprotective effects of curcumin are not well known. In the present study, the effect of curcumin on lipopolysaccharide (LPS)‑stimulated BV2 mouse microglia cells was investigated using enzyme‑linked immunosorbent assays of the culture medium and western blotting of cell lysates. The results showed that curcumin significantly inhibited the LPS‑induced expression and release of heat shock protein 60 (HSP60) in the BV2 cells. The level of heat shock factor (HSF)‑1 was upregulated in LPS‑activated BV2 microglia, indicating that the increased expression of HSP60 was driven by HSF‑1 activation. However, the increased HSF‑1 level was downregulated by curcumin. Extracellular HSP60 is a ligand of Toll‑like receptor 4 (TLR‑4), and the level of the latter was increased in the LPS‑activated BV2 microglia and inhibited by curcumin. The activation of TLR‑4 is known to be associated with the activation of myeloid differentiation primary response 88 (MyD88) and nuclear factor (NF)‑κB, with the subsequent production of proinflammatory and neurotoxic factors. In the present study, curcumin demonstrated marked suppression of the LPS‑induced expression of MyD88, NF‑κB, caspase‑3, inducible nitric oxide synthase, tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6 in the microglia. These results indicate that curcumin may exert its neuroprotective and anti‑inflammatory effects by inhibiting microglial activation through the HSP60/TLR‑4/MyD88/NF‑κB signaling wpathway. Therefore, curcumin may be useful for the treatment of neurodegenerative diseases that are associated with microglial activation.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Perry VH, Nicoll JA and Holmes C: Microglia in neurodegenerative disease. Nat Rev Neurol. 6:193–201. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Block ML, Zecca L and Hong JS: Microglia-mediated neurotoxicity: Uncovering the molecular mechanisms. Nature Rev Neurosci. 8:57–69. 2007. View Article : Google Scholar

3 

Hanisch UK and Kettenmann H: Microglia: Active sensor and versatile effector cells in the normal and pathologic brain. Nat Neurosci. 10:1387–1394. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Gehrmann J, Matsumoto Y and Kreutzberg GW: Microglia: Intrinsic immuneffector cell of the brain. Brain Res Brain Res Rev. 20:269–287. 1995. View Article : Google Scholar : PubMed/NCBI

5 

Lynch MA: The multifaceted profile of activated microglia. Mol Neurobiol. 40:139–156. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Li YH, Teng P, Wang Y, Zhang YM, Ma CJ and Pu J: Expression and regulation of HSP60 in activated microglia cells. Ningxia Yixueyuan Xuebao. 8:712–714. 2011.(In Chinese).

7 

Zhang D, Sun L, Zhu H, Wang L, Wu W, Xie J and Gu J: Microglial LOX-1 reacts with extracellular HSP60 to bridge neuroinflammation and neurotoxicity. Neurochem Int. 61:121–1035. 2012. View Article : Google Scholar

8 

Lehnardt S, Schott E, Trimbuch T, Laubisch D, Krueger C, Wulczyn G, Nitsch R and Werber JR: A vicious cycle involving release of heat shock protein 60 from injured cells and activation of toll-like receptor 4 mediates neurodegeneration in the CNS. J Neurosci. 28:2320–2331. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Motterlini R, Foresti R, Bassi R and Green CJ: Curcumin, an antioxidant and anti-inflammatory agent, induces heme oxygenase-1 and protects endothelial cells against oxidative stress. Free Radic Biol Med. 28:1303–1312. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Aggarwal BB and Harikumar KB: Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 41:40–59. 2009. View Article : Google Scholar : PubMed/NCBI

11 

De R, Kundu P, Swarnakar S, Ramamurthy T, Chowdhury A, Nair GB and Mukhopadhyay AK: Antimicrobial activity of curcumin against Helicobacter pylori isolates from India and during infections in mice. Antimicrob Agents Chemother. 53:1592–1597. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Piper JT, Singhal SS, Salameh MS, Torman RT, Awasthi YC and Awasthi S: Mechanisms of anticarcinogenic properties of curcumin: The effect of curcumin on glutathione linked detoxification enzymes in rat liver. Int J Biochem Cell Biol. 30:445–456. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Yue YK, Mo B, Zhao J, Yu YJ, Liu L, Yue CL and Liu W: Neuroprotective effect of curcumin against oxidative damage in BV-2 microglia and high intraocular pressure animal model. J Ocul Pharmacol Ther. 30:657–664. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Guo L, Xing Y, Pan R, Jiang M, Gong Z, Lin L, Wang J, Xiong G and Dong J: Curcumin protects microglia and primary rat cortical neurons against HIV-1 gp120-mediated inflammation and apoptosis. PLoS One. 8:e705652013. View Article : Google Scholar : PubMed/NCBI

15 

Mimeault M and Batra SK: Potential applications of curcumin and its novel synthetic analogs and nanotechnology-based formulations in cancer prevention and therapy. Chin Med. 6:1–19. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Kim SC, Stice JP, Chen L, Jung JS, Gupta S, Wang Y, Baumgarten G, Trial J and Knowlton AA: Extracellular heat shock protein 60, cardiac myocytes and apoptosis. Circ Res. 105:1186–1195. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Lin L, Kim SC, Wang Y, Gupta S, Davis B, Simon SI, Torre-Amione G and Knowlton AA: HSP60 in heart failure: Abnormal distribution and role in cardiac myocyte apoptosis. Am J Physiol Heart Circ Physiol. 293:H2238–H2247. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Youn HS, Saitoh SI, Miyake K and Hwang DH: Inhibition of homodimerization of Toll-like receptor 4 by curcumin. Biochem Pharmacol. 72:62–69. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Zeng Z, Zhan L, Liao H, Chen L and Lv X: Curcumin improves TNBS-induced colitis in rats by inhibiting IL-27 expression via the TLR4/NF-κB signaling pathway. Planta Med. 79:102–109. 2013.PubMed/NCBI

20 

Meng Z, Yan C, Deng Q, Gao DF and Niu XL: Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways. Acta Pharmacol Sin. 34:901–911. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Hansen JJ, Bross P, Westergaard M, Nielsen MN, Eiberg H, Børglum AD, Mogensen J, Kristiansen K, Bolund L and Gregersen N: Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter. Hum Genet. 112:71–77. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Soria JA, Arroyo DS, Gaviglio EA, Rodriguez-Galan MC, Wang JM and Iribarren P: Interleukin 4 induces the apoptosis of mouse microglial cells by a caspase-dependent mechanism. Neurobiol Dis. 43:616–624. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Burguillos MA, Deierborg T, Kavanagh E, Persson A, Hajji N, Garcia-Quintanilla A, Cano J, Brundin P, Englund E, Venero JL and Joseph B: Caspase signalling controls microglia activation and neurotoxicity. Nature. 472:319–324. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Kreutzberg GW: Microglia: A sensor for pathological events in the CNS. Trends Neurosci. 19:312–318. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Park E, Kim DY and Chun HS: Resveratrol inhibits lipopolysaccharide-induced phagocytotic activity in BV2 cells. J Korean Soc Appl Biol Chem. 55:803–807. 2012. View Article : Google Scholar

26 

Thanos S, Mey J and Wild M: Treatment of the adult retina with microglia-suppressing factors retards axotomy-induced neuronal degradation and enhances axonal regeneration in vivo and in vitro. J Neurosci. 13:455–466. 1993.PubMed/NCBI

27 

Teng P, Li YH, Cheng W, Zhou L, Shen Y and Wang Y: Neuroprotective effects of Lycium barbarum polysaccharides in lipopolysaccharide-induced BV2 microglial cells. Mol Med Rep. 7:1977–1981. 2013.PubMed/NCBI

28 

Ravindran J, Prasad S and Aggarwal BB: Curcumin and Cancer Cells: How Many Ways Can Curry Kill Tumor Cells Selectively? AAPS J. 11:495–510. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Cole GM, Teter B and Frautschy SA: Neuroprotective effects of curcumin. Adv Exp Med Biol. 595:197–212. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Mishra S and Palanivelu K: The effect of curcumin (turmeric) on Alzheimer's disease: An overview. Ann Indian Acad Neurol. 11:13–19. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Gong Z, Yang L, Tang H, Pan R, Xie S, Guo L, Wang J, Deng Q, Xiong G, Xing Y and Dong J: Protective effects of curcumin against human immunodeficiency virus 1 gp120 V3 loop-induced neuronal injury in rats. Neural Regen Res. 7:171–175. 2012.PubMed/NCBI

32 

Wang Y, Chen L, Hagiwara N and Knowlton AA: Regulation of heat shock protein 60 and 72 expression in the failing heart. J Mol Cell Cardiol. 48:360–366. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Ohashi K, Burkart V, Flohe S and Kolb H: Cutting edge: Heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex. J Immunol. 164:558–561. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Khasnavis S, Jana A, Roy A, Mazumder M, Bhushan B, Wood T, Ghosh S, Watson R and Pahan K: Suppression of nuclear factor-κB activation and inflammation in microglia by physically modified saline. J Biol Chem. 287:29529–29542. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Gu JH, Ge JB, Li M, Wu F, Zhang W and Qin ZH: Inhibition of NF-κB activation is associated with anti-inflammatory and anti-apoptotic effects of Ginkgolide B in a mouse model of cerebral ischemia/reperfusion injury. Eur J Pharm Sci. 47:652–660. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Kröncke KD, Fehsel K and Kolb-Bachofen V: Inducible nitric oxide synthase and its product nitric oxide, a small molecule with complex biological activities. Biol Chem Hoppe Seyler. 376:327–343. 1995.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ding F, Li F, Li Y, Hou X, Ma Y, Zhang N, Ma J, Zhang R, Lang B, Wang H, Wang H, et al: HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation. Exp Ther Med 12: 823-828, 2016.
APA
Ding, F., Li, F., Li, Y., Hou, X., Ma, Y., Zhang, N. ... Wang, Y. (2016). HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation. Experimental and Therapeutic Medicine, 12, 823-828. https://doi.org/10.3892/etm.2016.3413
MLA
Ding, F., Li, F., Li, Y., Hou, X., Ma, Y., Zhang, N., Ma, J., Zhang, R., Lang, B., Wang, H., Wang, Y."HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation". Experimental and Therapeutic Medicine 12.2 (2016): 823-828.
Chicago
Ding, F., Li, F., Li, Y., Hou, X., Ma, Y., Zhang, N., Ma, J., Zhang, R., Lang, B., Wang, H., Wang, Y."HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation". Experimental and Therapeutic Medicine 12, no. 2 (2016): 823-828. https://doi.org/10.3892/etm.2016.3413
Copy and paste a formatted citation
x
Spandidos Publications style
Ding F, Li F, Li Y, Hou X, Ma Y, Zhang N, Ma J, Zhang R, Lang B, Wang H, Wang H, et al: HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation. Exp Ther Med 12: 823-828, 2016.
APA
Ding, F., Li, F., Li, Y., Hou, X., Ma, Y., Zhang, N. ... Wang, Y. (2016). HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation. Experimental and Therapeutic Medicine, 12, 823-828. https://doi.org/10.3892/etm.2016.3413
MLA
Ding, F., Li, F., Li, Y., Hou, X., Ma, Y., Zhang, N., Ma, J., Zhang, R., Lang, B., Wang, H., Wang, Y."HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation". Experimental and Therapeutic Medicine 12.2 (2016): 823-828.
Chicago
Ding, F., Li, F., Li, Y., Hou, X., Ma, Y., Zhang, N., Ma, J., Zhang, R., Lang, B., Wang, H., Wang, Y."HSP60 mediates the neuroprotective effects of curcumin by suppressing microglial activation". Experimental and Therapeutic Medicine 12, no. 2 (2016): 823-828. https://doi.org/10.3892/etm.2016.3413
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team