AICAR reduces the collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: Suppression of HSP27 phosphorylation via p44/p42 MAP kinase

  • Authors:
    • Masanori Tsujimoto
    • Haruhiko Tokuda
    • Gen Kuroyanagi
    • Naohiro Yamamoto
    • Shingo Kainuma
    • Rie Matsushima‑Nishiwaki
    • Takashi Onuma
    • Yuko Iida
    • Akiko Kojima
    • Shigenobu Sawada
    • Tomoaki Doi
    • Yukiko Enomoto
    • Kumiko Tanabe
    • Shigeru Akamatsu
    • Hiroki Iida
    • Shinji Ogura
    • Takanobu Otsuka
    • Osamu Kozawa
    • Toru Iwama
  • View Affiliations

  • Published online on: June 7, 2016     https://doi.org/10.3892/etm.2016.3435
  • Pages: 1107-1112
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Abstract

We have previously reported that collagen-induced phosphorylation of heat shock protein (HSP) 27 via p44/p42 mitogen-activated protein (MAP) kinase in human platelets is sufficient to induce the secretion of platelet‑derived growth factor (PDGF)-AB and the release of soluble cluster of differentiation 40 ligand (sCD40L). Adenosine monophosphate-activated protein kinase (AMPK), which is known to regulate energy homeostasis, has a crucial role as an energy sensor in various eukaryotic cells. The present study investigated the effects of 5-aminoimidazole-4-carbox amide-1-β-d-ribofuranosyl 5'-monophosphate (AICAR), which is an activator of AMPK, on the collagen-induced activation of human platelets. It was demonstrated that AICAR dose‑dependently reduced collagen‑stimulated platelet aggregation up to 1.0 µM. Analysis of the size of platelet aggregates demonstrated that AICAR decreased the ratio of large aggregates (50‑70 µm), whereas the ratio of small aggregates (9-25 µm) was increased by AICAR administration. AICAR markedly attenuated the phosphorylation levels of p44/p42 MAP kinase and HSP27, which are induced by collagen. Furthermore, AICAR significantly decreased the secretion of PDGF‑AB and the collagen‑induced release of sCD40L. These results indicated that AICAR-activated AMPK may reduce the secretion of PDGF‑AB and the collagen‑induced release of sCD40L by inhibiting HSP27 phosphorylation via p44/p42 MAP kinase in human platelets.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tsujimoto M, Tokuda H, Kuroyanagi G, Yamamoto N, Kainuma S, Matsushima‑Nishiwaki R, Onuma T, Iida Y, Kojima A, Sawada S, Sawada S, et al: AICAR reduces the collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: Suppression of HSP27 phosphorylation via p44/p42 MAP kinase. Exp Ther Med 12: 1107-1112, 2016
APA
Tsujimoto, M., Tokuda, H., Kuroyanagi, G., Yamamoto, N., Kainuma, S., Matsushima‑Nishiwaki, R. ... Iwama, T. (2016). AICAR reduces the collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: Suppression of HSP27 phosphorylation via p44/p42 MAP kinase. Experimental and Therapeutic Medicine, 12, 1107-1112. https://doi.org/10.3892/etm.2016.3435
MLA
Tsujimoto, M., Tokuda, H., Kuroyanagi, G., Yamamoto, N., Kainuma, S., Matsushima‑Nishiwaki, R., Onuma, T., Iida, Y., Kojima, A., Sawada, S., Doi, T., Enomoto, Y., Tanabe, K., Akamatsu, S., Iida, H., Ogura, S., Otsuka, T., Kozawa, O., Iwama, T."AICAR reduces the collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: Suppression of HSP27 phosphorylation via p44/p42 MAP kinase". Experimental and Therapeutic Medicine 12.2 (2016): 1107-1112.
Chicago
Tsujimoto, M., Tokuda, H., Kuroyanagi, G., Yamamoto, N., Kainuma, S., Matsushima‑Nishiwaki, R., Onuma, T., Iida, Y., Kojima, A., Sawada, S., Doi, T., Enomoto, Y., Tanabe, K., Akamatsu, S., Iida, H., Ogura, S., Otsuka, T., Kozawa, O., Iwama, T."AICAR reduces the collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: Suppression of HSP27 phosphorylation via p44/p42 MAP kinase". Experimental and Therapeutic Medicine 12, no. 2 (2016): 1107-1112. https://doi.org/10.3892/etm.2016.3435