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Article

Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome

  • Authors:
    • Anush Martirosyan
    • Martin Petrek
    • Amit Kishore
    • Gayane Manukyan
  • View Affiliations / Copyright

    Affiliations: Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacky University, Olomouc 77520, Czech Republic
  • Pages: 1189-1195
    |
    Published online on: June 8, 2016
       https://doi.org/10.3892/etm.2016.3441
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Abstract

Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by thrombosis and recurrent fetal loss, with the persistent presence of antiphospholipid antibodies (aPLs). aPLs exert their pathogenic effect via the overproduction of tissue factor and activation of complement and several cell types, including endothelial cells, platelets and notably monocytes. As a result, a hypercoagulable state develops leading to APS‑associated obstetric complications and fetal loss. Despite being far from optimal, treatment of APS usually includes heparin and low dose aspirin. Recently, plasma exchange (PE) therapy was successfully used in patients with APS with obstetric complications who did not respond to the standard treatment. Therefore, the present study investigated the mechanism underlying PE action, and aimed to determine whether PE affects the functional activity of APS monocytes by examining the expression of 11 mRNA transcripts encoding cytokines, signaling molecules and transcription factors. Monocytes were collected prior to and following the PE treatment from women with APS who experienced recurrent pregnancy losses, as well as from healthy volunteers. Compared with control cells, APS monocytes showed deregulated expression of interleukin (IL)‑1β, IL‑6, IL‑23, chemokine (C‑C motif) ligand 2 (CCL2), C‑X‑C motif chemokine 10 (CXCL10), toll‑like receptor 2, and signal transducer and activator of transcription 3. PE treatment resulted in increased IL‑1β, IL‑6, IL‑23, CCL2, P2X7 and tumor necrosis factor‑α mRNA transcripts in APS monocytes, restoring the mRNA expression levels to within normal ranges. Furthermore, PE therapy counterbalanced the expression levels of CCL2 and CXCL10, the levels of which are indicative of T helper cell 1/2 balance. The results of the present study indicate that the altered transcriptional profile in APS monocytes was restored by the immunomodulatory effect of plasmapheresis.
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Copy and paste a formatted citation
Spandidos Publications style
Martirosyan A, Petrek M, Kishore A and Manukyan G: Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome. Exp Ther Med 12: 1189-1195, 2016.
APA
Martirosyan, A., Petrek, M., Kishore, A., & Manukyan, G. (2016). Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome. Experimental and Therapeutic Medicine, 12, 1189-1195. https://doi.org/10.3892/etm.2016.3441
MLA
Martirosyan, A., Petrek, M., Kishore, A., Manukyan, G."Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome". Experimental and Therapeutic Medicine 12.2 (2016): 1189-1195.
Chicago
Martirosyan, A., Petrek, M., Kishore, A., Manukyan, G."Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome". Experimental and Therapeutic Medicine 12, no. 2 (2016): 1189-1195. https://doi.org/10.3892/etm.2016.3441
Copy and paste a formatted citation
x
Spandidos Publications style
Martirosyan A, Petrek M, Kishore A and Manukyan G: Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome. Exp Ther Med 12: 1189-1195, 2016.
APA
Martirosyan, A., Petrek, M., Kishore, A., & Manukyan, G. (2016). Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome. Experimental and Therapeutic Medicine, 12, 1189-1195. https://doi.org/10.3892/etm.2016.3441
MLA
Martirosyan, A., Petrek, M., Kishore, A., Manukyan, G."Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome". Experimental and Therapeutic Medicine 12.2 (2016): 1189-1195.
Chicago
Martirosyan, A., Petrek, M., Kishore, A., Manukyan, G."Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome". Experimental and Therapeutic Medicine 12, no. 2 (2016): 1189-1195. https://doi.org/10.3892/etm.2016.3441
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