Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars

  • Authors:
    • Juan Zhao
    • Yan‑Chun Liu
    • Yan‑Hua Shi
    • Ya‑Qin Xie
    • Hai‑Peng Cui
    • Ying Li
    • Xiang‑Jun Li
    • Li‑Qun Ren
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China, Department of Experimental Pharmacology and Toxicology, Medical School of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 945-950
    |
    Published online on: June 8, 2016
       https://doi.org/10.3892/etm.2016.3442
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to provide reliable experimental evidence for the application of autologous skin fibroblasts (asFbs) in the repair of depressed scars. In the experiments, depressed trauma was induced in male Wistar rats, and fibroblasts were separated from the removed skin tissues to culture in medium. In vitro cultured asFbs were injected into the depressed scar sites of rats, and the repair function of asFbs in the depressed scars was then examined at the cellular and whole‑animal levels. The expression levels of type I and type III collagen in the dermal layer of the skin injected with asFb cells were significantly higher, as compared with those of the control, and type I collagen expression was significantly higher compared with Type III. Re‑injection of asFbs into the dermal layer of depressed scars can markedly improve their repair. These results may prove useful for skin repair in clinical settings.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Zeng W, Wei ZR, Liu D, Chai M and Zhao YM: Preliminary clinical observations on autologous cultured skin fibroblasts transplantation to treat the facial soft tissue deficiencies. Zhonghua Zheng Xing Wai Ke Za Zhi. 29:29–33. 2013.(In Chinese). PubMed/NCBI

2 

Ren HT, Hu H, Li Y, Jiang HF, Hu XL and Han CM: Endostatin inhibits hypertrophic scarring in a rabbit ear model. J Zhejiang Univ Sci B. 14:224–230. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Jung DH, Medikeri GS, Chang GU and Hyun SM: Surgical techniques for the correction of postrhinoplasty depressed scars on the nasal tip. JAMA Facial Plast Surg. 17:405–412. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Seok J, Choi SY, Park KY, Jang JH, Bae JH, Kim BJ, Kim MN and Hong CK: Depressed scar after filler injection successfully treated with pneumatic needleless injector and radiofrequency device. Dermatol Ther. 29:45–47. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Skigen AL, Bedrock RD and Stopperich PS: Correction of the depressed, retracted, post-tracheostomy scar. Plast Reconstr Surg. 103:1703–1705. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Inchingolo F, Tatullo M, Abenavoli FM, Marrelli M, Inchingolo AD, Corelli R, Inchingolo AM and Dipalma G: Surgical treatment of depressed scar: A simple technique. Int J Med Sci. 8:377–379. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Khan F, Richards K and Rashid RM: Hyaluronic acid filler for a depressed scar. Dermatol Online J. 18:152012.PubMed/NCBI

8 

Kubo K and Kuroyanagi Y: A study of cytokines released from fibroblasts in cultured dermal substitute. Artif Organs. 29:845–849. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Burd A and Chiu T: Allogeneic skin in the treatment of burns. Clin Dermatol. 23:376–387. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Chang H, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D and Brown PO: Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci USA. 99:12877–12882. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Clark DP, Hanke CW and Swanson NA: Dermal implants: Safety of products injected for soft tissue augmentation. J Am Aced Dermatol. 21:992–998. 1989. View Article : Google Scholar

12 

Viyoch J, Buranajaree S, Grandmottet F, Robin S, Binda D, Viennet C, Waranuch N and Humbert P: Evaluation of the effect of Thai breadfruit's heartwood extract on the biological functions of fibroblasts from wrinkles. J Cosmet Sci. 61:311–324. 2010.PubMed/NCBI

13 

Kotaska K, Petrak B, Kukacka J, Kraus J and Prusa R: Anti-vimentin antibodies and neuron-specific enolase in children with neurofibromatosis type-1. Neuro Endocrinol Lett. 28:761–764. 2007.PubMed/NCBI

14 

Chen X, Xu R, Jiang YN, Zhu WN and Wang YH: Simultaneous separation of primary cardiomyocytes and cardiac fibroblasts from neonatal rats with density gradient centrifugation. Sheng Li Xue Bao. 67:423–430. 2015.(In Chinese). PubMed/NCBI

15 

Avelar-Freitas BA, Almeida VG, Pinto MC, Mourão FA, Massensini AR, Martins-Filho OA, Rocha-Vieira E and Brito-Melo GE: Trypan blue exclusion assay by flow cytometry. Braz J Med Biol Res. 47:307–315. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Suto A, Kubota T, Shimoyama Y, Ishibiki K and Abe O: MTT assay with reference to the clinical effect of chemotherapy. J Surg Oncol. 42:28–32. 1989. View Article : Google Scholar : PubMed/NCBI

17 

Werely WA: Automated hematoxylin and eosin staining for large volumes of tissue. Am J Med Technol. 42:285–287. 1976.PubMed/NCBI

18 

Cooper JS and Lee BT: Treatment of facial scarring: lasers, filler, and nonoperative techniques. Facial Plast Surg. 25:311–315. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Han HH, Kim SY, Lee YJ, Moon SH and Oh DY: Donor-site closure using absorbable dermal staple for deep inferior epigastric artery perforator flaps: Its efficacy and cosmetic outcomes. Springerplus. 23:3632016. View Article : Google Scholar

20 

Byun SH, Ahn KM, Kim SM and Lee JH: Functional and cosmetic outcome after closure of radial forearm free flap donor defect with porcine collagen membrane. J Craniomaxillofac Surg. 44:527–532. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Tanaydin V, Conings J, Malyar M, van der Hulst R and van der Lei B: The role of topical vitamin E in scar management: A systematic review. Aesthet Surg J. Mar 14–2016.(Epub ahead of print) pii: sjw046. View Article : Google Scholar : PubMed/NCBI

22 

Munavalli GS, Smith S, Maslowski JM and Weiss RA: Successful treatment of depressed, distensible acne scars using autologous fibroblasts: A multi-site, prospective, double blind, placebo-controlled clinical trial. Dermatol Surg. 39:1226–1236. 2013. View Article : Google Scholar : PubMed/NCBI

23 

McAnulty RJ: Fibroblasts and myofibroblasts: Their source, function and role in disease. Int J Biochem Cell Biol. 39:666–671. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Pan S, Chen W, Liu X, Xiao J, Wang Y, Liu J, Du Y, Wang Y and Zhang Y: Application of a novel population of multipotent stem cells derived from skin fibroblasts as donor cells in bovine SCNT. PLoS One. 10:e01144232015. View Article : Google Scholar : PubMed/NCBI

25 

Doucet YS and Owens DM: Isolation and functional assessment of cutaneous stem cells. Methods Mol Biol. 1235:147–164. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Tondato F, Zeng H, Goodchild T, Ng FS, Chronos N and Peters NS: Autologous dermal fibroblast injections slow atrioventricular conduction and ventricular rate in atrial fibrillation in swine. Circ Arrhythm Electrophysiol. 8:439–446. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Walmsley GG, Maan ZN, Hu MS, Atashroo DA, Whittam AJ, Duscher D, TEvlin R, Marecic O, Lorenz HP, Gurtner GC and Longaker MT: Murine dermal fibroblast isolation by FACS. J Vis Exp. 107:e534302016.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Liu YC, Shi YH, Xie YQ, Cui HP, Li Y, Li XJ and Ren LQ: Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars. Exp Ther Med 12: 945-950, 2016.
APA
Zhao, J., Liu, Y., Shi, Y., Xie, Y., Cui, H., Li, Y. ... Ren, L. (2016). Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars. Experimental and Therapeutic Medicine, 12, 945-950. https://doi.org/10.3892/etm.2016.3442
MLA
Zhao, J., Liu, Y., Shi, Y., Xie, Y., Cui, H., Li, Y., Li, X., Ren, L."Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars". Experimental and Therapeutic Medicine 12.2 (2016): 945-950.
Chicago
Zhao, J., Liu, Y., Shi, Y., Xie, Y., Cui, H., Li, Y., Li, X., Ren, L."Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars". Experimental and Therapeutic Medicine 12, no. 2 (2016): 945-950. https://doi.org/10.3892/etm.2016.3442
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Liu YC, Shi YH, Xie YQ, Cui HP, Li Y, Li XJ and Ren LQ: Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars. Exp Ther Med 12: 945-950, 2016.
APA
Zhao, J., Liu, Y., Shi, Y., Xie, Y., Cui, H., Li, Y. ... Ren, L. (2016). Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars. Experimental and Therapeutic Medicine, 12, 945-950. https://doi.org/10.3892/etm.2016.3442
MLA
Zhao, J., Liu, Y., Shi, Y., Xie, Y., Cui, H., Li, Y., Li, X., Ren, L."Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars". Experimental and Therapeutic Medicine 12.2 (2016): 945-950.
Chicago
Zhao, J., Liu, Y., Shi, Y., Xie, Y., Cui, H., Li, Y., Li, X., Ren, L."Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars". Experimental and Therapeutic Medicine 12, no. 2 (2016): 945-950. https://doi.org/10.3892/etm.2016.3442
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team