Effect of adenovirus mediated β2-AR overexpression on IL-10 level secreted by cardiomyocytes of heart failure rats

Lin,Yan; Zheng,Cheng; Liu,Ying; Wang,Lei; Gong,Haibin

doi:10.3892/etm.2016.3451

Effect of adenovirus mediated β2-AR overexpression on IL-10 level secreted by cardiomyocytes of heart failure rats

Authors:
- Yan Lin
- Cheng Zheng
- Ying Liu
- Lei Wang
- Haibin Gong
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Affiliations: Xuzhou Central Hospital, Xuzhou Cardiovascular Disease Institute, Xuzhou, Jiangsu 221009, P.R. China
Published online on: June 13, 2016 https://doi.org/10.3892/etm.2016.3451
Pages: 1349-1354
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effect of β2-adrenergic receptor (AR) overexpression on interleukin (IL)-10 content secreted by cardiomyocytes of heart failure (HF) rats was investigated. A rat model of chronic HF was established by partially banding abdominal aorta and the cardiomyocytes were isolated with collagenase II. The cardiomyocytes were then transfected with adenovirus type 5-ADRβ2-enhanced green fluorescent protein (EGFP) for 48 h to observe the changes of β2‑AR protein expression using western blot analysis. The IL‑10 level was detected by ELISA. The experiment was divided into seven groups: Control, HF, HF+EGFP, HF+β2, sham, sham+EGFP and sham+β2 groups. Compared with the sham‑operated group, left ventricular diastolic dimension, and left ventricular systolic dimension were increased (P<0.05), whereas ejection fraction and fractional shortening were decreased (P<0.05) in the HF group. Compared with the sham group, the cardiomyocyte survival rate of the HF group was significantly reduced (P<0.05). Compared with the control or sham group, the β2‑AR protein level of the HF group showed no significant differences (P>0.05). Compared with the HF and HF+EGFP groups, the expression of β2‑AR protein of cardiomyocytes was increased in the HF+β2 group (P<0.05). Compared with the sham group, IL‑10 content secreted by cardiomyocytes in the HF group was increased (P<0.05). Compared with the HF and HF+EGFP groups, IL‑10 content in the HF+β2 group was increased significantly (P<0.05). In conclusion, the concentration of IL‑10 secreted by cardiomyocytes of HF rats was increased. The overexpression of β2‑AR in the cardiomyocytes of HF rats was able to enhance the secretion of IL-10.

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