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Article

Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension

  • Authors:
    • Min Shi
    • Jue Wei
    • Wen‑Ying Meng
    • Na Wang
    • Ting Wang
    • Yu‑Gang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Shanghai Tongren Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200336, P.R. China
  • Pages: 1618-1624
    |
    Published online on: June 14, 2016
       https://doi.org/10.3892/etm.2016.3454
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Abstract

The current study investigated the effects of phased joint intervention on clinical efficacy and Rho/Rho-associated coil protein kinase (ROCK) expression in patients with portal hypertension complicated by esophageal variceal bleeding (EVB) and hypersplenism. Patients with portal hypertension (n=53) caused by liver cirrhosis complicated by EVB and hypersplenism treated with phased joint intervention were assessed, and portal hemodynamics, blood, liver function, complications, and rebleeding incidence were analyzed. Reverse transcription‑quantitative polymerase chain reaction was used to measure Rho, ROCK1 and ROCK2 mRNA expression levels in peripheral blood mononuclear cells prior to and following phased joint intervention, and western blotting was employed to determine the protein expression levels of Rho, ROCK1, ROCK2, phosphorylated (p) myosin phosphatase target subunit 1 (MYPT1) and total‑MYPT1. All patients underwent an emergency assessment of hemostasis with a 100% success rate. Varicose veins were alleviated, and portal hemodynamics and liver function improved following intervention. Furthermore, preoperative and postoperative expression levels of Rho, ROCK1 and ROCK2 mRNA were higher compared with the control group. Notably, the mRNA expression levels of Rho, ROCK1 and ROCK2 in the postoperative group were significantly lower when compared with the preoperative group. Protein expression levels of Rho, ROCK1, ROCK2 and pMYPT1 in the postoperative group were lower, as compared with the preoperative group. Concentration levels of transforming growth factor‑β1, connective tissue growth factor and platelet‑derived growth factor in peripheral blood were significantly reduced following phased joint intervention. Therefore, the present findings demonstrated that phased joint intervention is able to effectively treat EVB and hypersplenism, and improve liver function. The efficacy of phased joint intervention may be associated with its role in the regulation of the Rho-ROCK signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Shi M, Wei J, Meng WY, Wang N, Wang T and Wang YG: Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension. Exp Ther Med 12: 1618-1624, 2016.
APA
Shi, M., Wei, J., Meng, W., Wang, N., Wang, T., & Wang, Y. (2016). Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension. Experimental and Therapeutic Medicine, 12, 1618-1624. https://doi.org/10.3892/etm.2016.3454
MLA
Shi, M., Wei, J., Meng, W., Wang, N., Wang, T., Wang, Y."Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension". Experimental and Therapeutic Medicine 12.3 (2016): 1618-1624.
Chicago
Shi, M., Wei, J., Meng, W., Wang, N., Wang, T., Wang, Y."Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1618-1624. https://doi.org/10.3892/etm.2016.3454
Copy and paste a formatted citation
x
Spandidos Publications style
Shi M, Wei J, Meng WY, Wang N, Wang T and Wang YG: Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension. Exp Ther Med 12: 1618-1624, 2016.
APA
Shi, M., Wei, J., Meng, W., Wang, N., Wang, T., & Wang, Y. (2016). Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension. Experimental and Therapeutic Medicine, 12, 1618-1624. https://doi.org/10.3892/etm.2016.3454
MLA
Shi, M., Wei, J., Meng, W., Wang, N., Wang, T., Wang, Y."Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension". Experimental and Therapeutic Medicine 12.3 (2016): 1618-1624.
Chicago
Shi, M., Wei, J., Meng, W., Wang, N., Wang, T., Wang, Y."Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertension". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1618-1624. https://doi.org/10.3892/etm.2016.3454
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