Effect of baicalin on hippocampal damage in kainic acid-induced epileptic mice

Liao,Zheng‑Jian; Liang,Ri‑Sheng; Shi,Song‑Sheng; Wang,Chun‑Hua; Yang,Wei‑Zhong

doi:10.3892/etm.2016.3461

Effect of baicalin on hippocampal damage in kainic acid-induced epileptic mice

Authors:
- Zheng‑Jian Liao
- Ri‑Sheng Liang
- Song‑Sheng Shi
- Chun‑Hua Wang
- Wei‑Zhong Yang
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Affiliations: Department of Neurosurgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China
Published online on: June 16, 2016 https://doi.org/10.3892/etm.2016.3461
Pages: 1405-1411
Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to determine the effect of baicalin on the expression of miR-497 and its target B‑cell lymphoma-2 (Bcl-2) in the hippocampus of kainic acid (KA)-induced epileptic mice. To establish status epilepticus (SE), 0.1 µg/5 µl KA was injected into the lateral cerebral ventricle in mice, which then received an intraperitoneal injection of baicalin (100 mg/kg) after 1 and 8 h. Hematoxylin and eosin staining was used to observe the pathological changes in morphology and neuronal apoptosis was determined by terminal transferase‑mediated dUTP nick end‑labeling staining. Western blot analysis was used to detect the expression of Bcl‑2 and cleaved caspase‑3 proteins in the hippocampus, while reverse transcription‑quantitative polymerase chain reaction was used to quantify hippocampal miR‑497 expression. The results showed that baicalin significantly attenuated neuronal damage and apoptosis in the hippocampus 72 h after SE. In addition, baicalin decreased SE‑induced expression of miR‑497 and cleaved caspase‑3 protein, while upregulating the expression of Bcl‑2 protein. In conclusion, the present results suggest that baicalin possesses potent antiapoptotic properties and attenuates hippocampal injury in mice after SE, which may be associated with the downregulation of miR‑497 and cleaved caspase‑3 and the upregulation of Bcl-2.

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