Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression

  • Authors:
    • Xiaoliang Shu
    • Jian Zhang
    • Qingxiu Wang
    • Zengguang Xu
    • Tingting Yu
  • View Affiliations / Copyright

    Affiliations: Department of Nutrition, Jinshan Hospital, Fudan University School of Medicine, Shanghai 201508, P.R. China, Department of Anesthesiology, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China, Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China
    Copyright: © Shu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1367-1372
    |
    Published online on: June 17, 2016
       https://doi.org/10.3892/etm.2016.3468
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Intestinal ischemia-reperfusion (IR) is a common clinical pathophysiological process that is common in severe trauma, major surgery, and in post-resuscitation. Glutamine (Gln) reduces intestinal IR injury, however, its mechanism of action remains to be determined. High mobility group box 1 (HMGB1) protein, nuclear factor-κB (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-1 (IL-1) are mediators involved in the pathophysiology of intestinal IR injury. The aim of the present study was to investigate the effects of Gln on the intestinal mucosa of HMGB1 expression following IR to determine whether Gln relieved intestinal IR injury in the intestinal mucosal barrier. Forty‑eight Sprague‑Dawley rats were included in the present study. A model of intestinal ischemia-reperfusion injury was established by clamping the superior mesenteric artery of the rats to cause ischemia, followed by restoring blood flow. The animals were randomly divided into the control (n=24) and the Gln (n=24) groups for the experiments. The two groups of rats were given enteral nutrition with equal heat, nitrogen (heat 125.4 kJ/kg/day, nitrogen 0.2 g/kg/day). The Gln group of rats was fed with enteral nutrition plus 3% Gln, while the control rats were fed with enteral nutrition plus 3% soybean protein. After 7 days, the HMGB1 and plasma levels of NF-κB, TNF-α, IL-1, Gln, D-lactic acid and diamine oxidase (DAO) were observed. The changes in the morphology of intestinal mucosa were observed using electron microscopy. The plasma levels of TNF-α, IL-1, D-lactic acid and DAO, and the level of HMGB1, NF-κB, TNF-α and IL-1 in intestinal mucosa were significantly higher after IR (p<0.05), while the plasma level of Gln was lower in the two groups. In the control group, the plasma level of IL-1, TNF-α, DAO and D-lactic acid, and that of HMGB1, NF-κB, TNF-α, and IL-1 in intestinal mucosa were significantly higher, while the plasma level of Gln was lower than that prior to modeling on the 3rd and 7th days of the experiment. In the Gln group, the plasma level of IL-1, TNF-α, DAO and D-lactic acid, and that of HMGB1, NF-κB, IL-1, and TNF-α in intestinal mucosa were significantly higher (p<0.05) compared to the control on the 3rd and 7th days of the experiment. By contrast, after the 7th day, the plasma level of IL-1, TNF-α, DAO and D-lactic acid, and the level of HMGB1, NF-κB, IL-1, TNF-α in intestinal mucosa were significantly lower in the Gln group, while the plasma level of Gln was significantly higher than those in control group and after IR on the 7th day of the experiment. Additionally, the structure of villi and recess was damaged, villi was sparse and short, and considerable inflammatory cell influx embellished the lamina propria, lymphangiectasia, and edema after IR. On the 7th day, compared to after IR, the intestinal villi and recess structure of the controls was significantly restored in the Gln group. In conclusion, Gln repaired the intestinal mucosal injury in IR by reducing the expression of HMGB1 and inflammatory cytokines, and reducing the permeability of the intestinal mucosa.
View Figures

Figure 1

View References

1 

Mura M, Andrade CF, Han B, Seth R, Zhang Y, Bai XH, Waddell TK, Hwang D, Keshavjee S and Liu M: Intestinal ischemia-reperfusion-induced acute lung injury and oncotic cell death in multiple organs. Shock. 28:227–238. 2007. View Article : Google Scholar : PubMed/NCBI

2 

He GZ, Dong LG, Chen XF, Zhou KG and Shu H: Lymph duct ligation during ischemia/reperfusion prevents pulmonary dysfunction in a rat model with ω-3 polyunsaturated fatty acid and glutamine. Nutrition. 27:604–614. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Collange O, Tamion F, Chanel S, Hue G, Richard V, Thuilliez C, Dureuil B and Plissonnier D: D-lactate is not a reliable marker of gut ischemia-reperfusion in a rat model of supraceliac aortic clamping. Crit Care Med. 34:1415–1419. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Wischmeyer PE: Glutamine: role in gut protection in critical illness. Curr Opin Clin Nutr Metab Care. 9:607–612. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Songsasen N, Wesselowski S, Carpenter JW and Wildt DE: The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation. Mol Reprod Dev. 79:186–196. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Barnes JL, Hartmann B, Holst JJ and Tappenden KA: Intestinal adaptation is stimulated by partial enteral nutrition supplemented with the prebiotic short-chain fructooligosaccharide in a neonatal intestinal failure piglet model. JPEN J Parenter Enteral Nutr. 36:524–537. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Padda RS, Gkouvatsos K, Guido M, Mui J, Vali H and Pantopoulos K: A high-fat diet modulates iron metabolism but does not promote liver fibrosis in hemochromatotic Hjv−/− mice. Am J Physiol Gastrointest Liver Physiol. 308:G251–G261. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Wen AD, Jiang YP and Fan YX: Using HPLC fluorescence method for rapid detection of glutamine in human plasma and muscle. Chromatography. 406–407. 1995.

9 

Kim KH, Kuh SU, Park JY, Kim KS, Chin DK and Cho YE: What is the importance of ‘halo’ phenomenon around bone cement following vertebral augmentation for osteoporotic compression fracture? Osteoporos Int. 23:2559–2565. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Tian R, Tan JT, Wang RL, Xie H, Qian YB and Yu KL: The role of intestinal mucosa oxidative stress in gut barrier dysfunction of severe acute pancreatitis. Eur Rev Med Pharmacol Sci. 17:349–355. 2013.PubMed/NCBI

11 

Chen X, Guan T, Li C, Shang H, Cui L, Li XM and Kong J: SOD1 aggregation in astrocytes following ischemia/reperfusion injury: a role of NO-mediated S-nitrosylation of protein disulfide isomerase (PDI). J Neuroinflammation. 9:2372012. View Article : Google Scholar : PubMed/NCBI

12 

Alves MA, Guimarães SB, Dias DA and Vasconcelos PR, Coelho VP and Vasconcelos PR: Effects of L-alanyl-glutamine upon the blood and kidney biochemical parameters in the rat hind limb model of ischemia/reperfusion. Acta Cir Bras. 20:445–449. 2005. View Article : Google Scholar : PubMed/NCBI

13 

De-Souza DA and Greene LJ: Intestinal permeability and systemic infections in critically ill patients: effect of glutamine. Crit Care Med. 33:1125–1135. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Sözen S, Topuz O, Uzun AS, Cetinkünar S and Das K: Prevention of bacterial translocation using glutamine and melatonin in small bowel ischemia and reperfusion in rats. Ann Ital Chir. 83:143–148. 2012.PubMed/NCBI

15 

Deniel N, Marion-Letellier R, Charlionet R, Tron F, Leprince J, Vaudry H, Ducrotté P, Déchelotte P and Thébault S: Glutamine regulates the human epithelial intestinal HCT-8 cell proteome under apoptotic conditions. Mol Cell Proteomics. 6:1671–1679. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Yeh SL, Lai YN, Shang HF, Lin MT, Chiu WC and Chen WJ: Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis. World J Gastroenterol. 11:1742–1746. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Kessel A, Toubi E, Pavlotzky E, Mogilner J, Coran AG, Lurie M, Karry R and Sukhotnik I: Treatment with glutamine is associated with down-regulation of Toll-like receptor-4 and myeloid differentiation factor 88 expression and decrease in intestinal mucosal injury caused by lipopolysaccharide endotoxaemia in a rat. Clin Exp Immunol. 151:341–347. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Filipp FV, Ratnikov B, De Ingeniis J, Smith JW, Osterman AL and Scott DA: Glutamine-fueled mitochondrial metabolism is decoupled from glycolysis in melanoma. Pigment Cell Melanoma Res. 25:732–739. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Naruse K, Sado T, Noguchi T, Tsunemi T, Yoshida S, Akasaka J, Koike N, Oi H and Kobayashi H: Peripheral RAGE (receptor for advanced glycation endproducts)-ligands in normal pregnancy and preeclampsia: novel markers of inflammatory response. J Reprod Immunol. 93:69–74. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Hedl M and Abraham C: Nod2-induced autocrine interleukin-1 alters signaling by ERK and p38 to differentially regulate secretion of inflammatory cytokines. Gastroenterology. 143:1530–1543. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Kang J, Tae N, Min BS, Choe J and Lee JH: Malabaricone C suppresses lipopolysaccharide-induced inflammatory responses via inhibiting ROS-mediated Akt/IKK/NF-κB signaling in murine macrophages. Int Immunopharmacol. 14:302–310. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Karatepe O, Acet E, Battal M, Adas G, Kemik A, Altiok M, Kamali G, Koculu S, Catay A, Kamali S, et al: Effects of glutamine and curcumin on bacterial translocation in jaundiced rats. World J Gastroenterol. 16:4313–4320. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Websky M, Fujishiro J, Ohsawa I, Praktiknjo M, Wehner S, Abu-Elmagd K, Kitamura K, Kalff JC, Schaefer N and Pech T: The novel guanylhydrazone CPSI-2364 ameliorates ischemia reperfusion injury after experimental small bowel transplantation. Transplantation. 95:1315–1323. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Pan ZY, Long CL and Wang H: Functional and morphological structure changes in the gut barrier during cholinesterase inhibitor intoxication and therapeutic effect of benthiactzine in rats. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 22:197–200. 2010.(In Chinese). PubMed/NCBI

25 

Cai C, Li W, Chen J, Li X and Chen S: Diamine oxidase as a marker for diagnosis of superior mesenteric arterial occlusion. Hepatogastroenterology. 59:155–158. 2012.PubMed/NCBI

26 

Santos RG, Quirino IE, Viana ML, Generoso SV, Nicoli JR, Martins FS, Nogueira-Machado JA, Arantes RM, Correia MI and Cardoso VN: Effects of nitric oxide synthase inhibition on glutamine action in a bacterial translocation model. Br J Nutr. 111:93–100. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shu X, Zhang J, Wang Q, Xu Z and Yu T: Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression. Exp Ther Med 12: 1367-1372, 2016.
APA
Shu, X., Zhang, J., Wang, Q., Xu, Z., & Yu, T. (2016). Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression. Experimental and Therapeutic Medicine, 12, 1367-1372. https://doi.org/10.3892/etm.2016.3468
MLA
Shu, X., Zhang, J., Wang, Q., Xu, Z., Yu, T."Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression". Experimental and Therapeutic Medicine 12.3 (2016): 1367-1372.
Chicago
Shu, X., Zhang, J., Wang, Q., Xu, Z., Yu, T."Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1367-1372. https://doi.org/10.3892/etm.2016.3468
Copy and paste a formatted citation
x
Spandidos Publications style
Shu X, Zhang J, Wang Q, Xu Z and Yu T: Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression. Exp Ther Med 12: 1367-1372, 2016.
APA
Shu, X., Zhang, J., Wang, Q., Xu, Z., & Yu, T. (2016). Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression. Experimental and Therapeutic Medicine, 12, 1367-1372. https://doi.org/10.3892/etm.2016.3468
MLA
Shu, X., Zhang, J., Wang, Q., Xu, Z., Yu, T."Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression". Experimental and Therapeutic Medicine 12.3 (2016): 1367-1372.
Chicago
Shu, X., Zhang, J., Wang, Q., Xu, Z., Yu, T."Glutamine decreases intestinal mucosal injury in a rat model of intestinal ischemia-reperfusion by downregulating HMGB1 and inflammatory cytokine expression". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1367-1372. https://doi.org/10.3892/etm.2016.3468
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team