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Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage

  • Authors:
    • Qing‑Zhen Liu
    • Xu‑Dong Chen
    • Gang Liu
    • Guang‑Ju Guan
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Respiratory Medicine, Shandong Jiaotong Hospital, Jinan, Shandong 250031, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1651-1656
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    Published online on: July 13, 2016
       https://doi.org/10.3892/etm.2016.3516
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Abstract

Adult stem cells have been well characterized in numerous organs, with the exception of the kidneys. Therefore, the present study aimed to identify and isolate kidney‑derived stem cells. A total of 12 Fischer 344 transgenic rats expressing the human diphtheria toxin receptor in podocyte cells of the kidney, were used in the present study. The rats were administered 5-bromo‑2'-deoxyuridine (BrdU) in order to detect cellular proliferation. After 60 days, the rats were treated with the diphtheria toxin (DT), in order to induce kidney injury. Immunohistochemical analysis indicated that the number of BrdU‑positive cells were increased following DT treatment. In addition, the expression of octamer-binding transcription factor 4 (Oct‑4), a stem cell marker, was detected and suggested that kidney‑specific stem cells were present in the DT‑treated tissue samples. Furthermore, tissue samples exhibited repair of the DT‑induced injury. Further cellular culturing was conducted in order to isolate the kidney‑specific stem cells. After 5 weeks of culture, the majority of the cells were non‑viable, with the exception of certain specialized, unique cell types, which were monomorphic and spindle‑shaped in appearance. The unique cells were isolated and subjected to immunostaining and reverse transcription‑polymerase chain reaction analyses in order to reconfirm the expression of Oct-4 and to detect the expression of Paired box 2 (Pax‑2), which is necessary for the formation of kidney structures. The unique cells were positive for Oct-4 and Pax-2; thus suggesting that the identified cells were kidney‑derived stem cells. The results of the present study suggested that the unique cell type identified in the kidneys of the DT‑treated rats were kidney‑specific stem cells that may have been involved in the repair of DT‑induced tissue injury. In addition, these cells may provide a useful cell line for studying the fundamental characteristics of kidney stem cells, as well as identifying kidney‑specific stem cell markers.
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1 

Lindvall O, Kokaia Z and Martinez-Serrano A: Stem cell therapy for human neurodegenerative disorders - how to make it work. Nat Med. 10:S42–S50. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Segers VFM and Lee RT: Stem-cell therapy for cardiac disease. Nature. 451:937–942. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Weissman IL: Stem cells: Units of development, units of regeneration and units in evolution. Cell. 100:157–168. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Cotsarelis G, Cheng SZ, Dong G, Sun TT and Lavker RM: Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: Implications on epithelial stem cells. Cell. 57:201–209. 1989. View Article : Google Scholar : PubMed/NCBI

5 

Taylor G, Lehrer MS, Jensen PJ, Sun TT and Lavker RM: Involvement of follicular stem cells in forming not only the follicle but also the epidermis. Cell. 102:451–461. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Lavker RM and Sun TT: Epidermal stem cells: Properties, markers, and location. Proc Natl Acad Sci USA. 97:13473–13475. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Beltrami AP, Barlucchi L, Torella D, Baker M, Limana F, Chimenti S, Kasahara H, Rota M, Musso E, Urbanek K, et al: Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell. 114:763–776. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Alison MR, Poulsom R and Forbes SJ: Update on hepatic stem cells. Liver. 21:367–373. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Bernard-Kargar C and Ktorza A: Endocrine pancreas plasticity under physiological and pathological conditions. Diabetes. 50(Suppl 1): S30–S35. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Forbes SJ, Poulsom R and Wright NA: Hepatic and renal differentiation from blood-borne stem cells. Gene Ther. 9:625–630. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Morrison SJ, White PM, Zock C and Anderson DJ: Prospective identification, isolation by flow cytometry and in vivo self-renewal of multipotent mammalian neural crest stem cells. Cell. 96:737–749. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Wright NA: Epithelial stem cell repertoire in the gut: Clues to the origin of cell lineages, proliferative units and cancer. Int J Exp Pathol. 81:117–143. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Gupta S and Rosenberg ME: Do stem cells exist in the adult kidney? Am J Nephrol. 28:607–613. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Drummond IA, Mukhopadhyay D and Sukhatme VP: Expression of fetal kidney growth factors in a kidney tumor line: Role of FGF2 in kidney development. Exp Nephrol. 6:522–533. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Elger M, Hentschel H, Litteral J, Wellner M, Kirsch T, Luft FC and Haller H: Nephrogenesis is induced by partial nephrectomy in the elasmobranch Leucoraja erinacea. J Am Soc Nephrol. 14:1506–1518. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Salice CJ, Rokous JS, Kane AS and Reimschuessel R: New nephron development in goldfish (Carassius auratus) kidneys following repeated gentamicin-induced nephrotoxicosis. Comp Med. 51:56–59. 2001.PubMed/NCBI

17 

Poulsom R, Forbes SJ, Hodivala-Dilke K, Ryan E, Wyles S, Navaratnarasah S, Jeffery R, Hunt T, Alison M, Cook T, et al: Bone marrow contributes to renal parenchymal turnover and regeneration. J Pathol. 195:229–235. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Gupta S, Verfaillie C, Chmielewski D, Kim Y and Rosenberg ME: A role for extrarenal cells in the regeneration following acute renal failure. Kidney Int. 62:1285–1290. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Lin F, Cordes K, Li L, Hood L, Couser WG, Shankland SJ and Igarashi P: Hematopoietic stem cells contribute to the regeneration of renal tubules after renal ischemia-reperfusion injury in mice. J Am Soc Nephrol. 14:1188–1199. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Kale S, Karihaloo A, Clark PR, Kashgarian M, Krause DS and Cantley LG: Bone marrow stem cells contribute to repair of the ischemically injured renal tubule. J Clin Invest. 112:42–49. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Szczypka MS, Westover AJ, Clouthier SG, Ferrara JL and Humes HD: Rare incorporation of bone marrow-derived cells into kidney after folic acid-induced injury. Stem Cells. 23:44–54. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Herzlinger D, Koseki C, Mikawa T and Al-Awqati Q: Metanephric mesenchyme contains multipotent stem cells whose fate is restricted after induction. Development. 114:565–572. 1992.PubMed/NCBI

23 

Oliver JA, Maarouf O, Cheema FH, Martens TP and Al-Awqati Q: The renal papilla is a niche for adult kidney stem cells. J Clin Invest. 114:795–804. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Dyce PW, Zhu H, Craig J and Li J: Stem cells with multilineage potential derived from porcine skin. Biochem Biophys Res Commun. 316:651–658. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Yeom YI, Fuhrmann G, Ovitt CE, Brehm A, Ohbo K, Gross M, Hübner K and Schöler HR: Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells. Development. 122:881–894. 1996.PubMed/NCBI

26 

Mitalipov SM, Kuo HC, Hennebold JD and Wolf DP: Oct-4 expression in pluripotent cells of the rhesus monkey. Biol Reprod. 69:1785–1792. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Kehler J, Tolkunova E, Koschorz B, Pesce M, Gentile L, Boiani M, Lomelí H, Nagy A, McLaughlin KJ, Schöler HR and Tomilin A: Oct4 is required for primordial germ cell survival. EMBO Rep. 5:1078–1083. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Jiang Y, Vaessen B, Lenvik T, Blackstad M, Reyes M and Verfaillie CM: Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle and brain. Exp Hematol. 30:896–904. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, et al: Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 418:41–49. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Schwartz RE, Reyes M, Koodie L, Jiang Y, Blackstad M, Lund T, Lenvik T, Johnson S, Hu WS and Verfaillie CM: Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest. 109:1291–1302. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Reyes M, Dudek A, Jahagirdar B, Koodie L, Marker PH and Verfaillie CM: Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest. 109:337–346. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M and Xu X: Isolation of a novel population of multipotent adult stem cells from human hair follicles. Am J Pathol. 168:1879–1888. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Baal N, Reisinger K, Jahr H, Bohle RM, Liang O, Münstedt K, Rao CV, Preissner KT and Zygmunt MT: Expression of transcription factor Oct-4 and other embryonic genes in CD133 positive cells from human umbilical cord blood. Thromb Haemost. 92:767–775. 2004.PubMed/NCBI

34 

D'Ippolito G, Diabira S, Howard GA, Menei P, Roos BA and Schiller PC: Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential. J Cell Sci. 117:2971–2981. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Davis SF, Hood J, Thomas A and Bunnell BA: Isolation of adult rhesus neural stem and progenitor cells and differentiation into immature oligodendrocytes. Stem Cells Dev. 15:191–199. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Romagnani P, Annunziato F, Liotta F, Lazzeri E, Mazzinghi B, Frosali F, Cosmi L, Maggi L, Lasagni L, Scheffold A, et al: CD14+ CD34 low cells with stem cell phenotypic and functional features are the major source of circulating endothelial progenitors. Circ Res. 97:314–322. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Romero-Ramos M, Vourc'h P, Young HE, Lucas PA, Wu Y, Chivatakarn O, Zaman R, Dunkelman N, el-Kalay MA and Chesselet MF: Neuronal differentiation of stem cells isolated from adult muscle. J Neurosci Res. 69:894–907. 2002. View Article : Google Scholar : PubMed/NCBI

38 

Trosko JE and Tai MH: Adult stem cell theory of the multi-stage, multi-mechanism theory of carcinogenesis: Role of inflammation on the promotion of initiated stem cells. Contrib Microbiol. 13:45–65. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Tsai MS, Hwang SM, Tsai YL, Cheng FC, Lee JL and Chang YJ: Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells. Biol Reprod. 74:545–551. 2006. View Article : Google Scholar : PubMed/NCBI

40 

Xiao J, Nan Z, Motooka Y and Low WC: Transplantation of a novel cell line population of umbilical cord blood stem cells ameliorates neurological deficits associated with ischemic brain injury. Stem Cells Dev. 14:722–733. 2005. View Article : Google Scholar : PubMed/NCBI

41 

Zhou YF, Fang F, Fu JR, Dong YS, Ye DY, Shu SN, Zhen H and Li G: An experimental study on astrocytes promoting production of neural stem cells derived from mouse embryonic stem cells. Chin Med J (Engl). 118:1994–1999. 2005.PubMed/NCBI

42 

Pappenheimer AM Jr: The story of a toxic protein, 1888–1992. Protein Sci. 2:292–298. 1993. View Article : Google Scholar : PubMed/NCBI

43 

Wharram BL, Goyal M, Wiggins JE, Sanden SK, Hussain S, Filipiak WE, Saunders TL, Dysko RC, Kohno K, Holzman LB and Wiggins RC: Podocyte depletion causes glomerulosclerosis: Diphtheria toxin-induced podocyte depletion in rats expressing human diphtheria toxin receptor transgene. J Am Soc Nephrol. 16:2941–2952. 2005. View Article : Google Scholar : PubMed/NCBI

44 

Maeshima A, Sakurai H and Nigam SK: Adult kidney tubular cell population showing phenotypic plasticity, tubulogenic capacity and integration capability into developing kidney. J Am Soc Nephrol. 17:188–198. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Gupta S, Verfaillie C, Chmielewski D, Kren S, Eidman K, Connaire J, Heremans Y, Lund T, Blackstad M, Jiang Y, et al: Isolation and characterization of kidney-derived stem cells. J Am Soc Nephrol. 17:3028–3040. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu QZ, Chen XD, Liu G and Guan GJ: Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage. Exp Ther Med 12: 1651-1656, 2016.
APA
Liu, Q., Chen, X., Liu, G., & Guan, G. (2016). Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage. Experimental and Therapeutic Medicine, 12, 1651-1656. https://doi.org/10.3892/etm.2016.3516
MLA
Liu, Q., Chen, X., Liu, G., Guan, G."Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage". Experimental and Therapeutic Medicine 12.3 (2016): 1651-1656.
Chicago
Liu, Q., Chen, X., Liu, G., Guan, G."Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1651-1656. https://doi.org/10.3892/etm.2016.3516
Copy and paste a formatted citation
x
Spandidos Publications style
Liu QZ, Chen XD, Liu G and Guan GJ: Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage. Exp Ther Med 12: 1651-1656, 2016.
APA
Liu, Q., Chen, X., Liu, G., & Guan, G. (2016). Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage. Experimental and Therapeutic Medicine, 12, 1651-1656. https://doi.org/10.3892/etm.2016.3516
MLA
Liu, Q., Chen, X., Liu, G., Guan, G."Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage". Experimental and Therapeutic Medicine 12.3 (2016): 1651-1656.
Chicago
Liu, Q., Chen, X., Liu, G., Guan, G."Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1651-1656. https://doi.org/10.3892/etm.2016.3516
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