Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor

  • Authors:
    • Xiaofeng Zhou
    • Guoqiang Cai
    • Yi He
    • Guotong Tong
  • View Affiliations

  • Published online on: July 20, 2016     https://doi.org/10.3892/etm.2016.3536
  • Pages: 1812-1816
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cordycepin exhibits various bio-activities, including anticancer, antibacterial, antiviral and immune regulation activities, and is a significant focus of research. However, the preparation of high-purity cordycepin remains challenging. Also, the molecular target with which cordycepin interacts to cause an antibacterial effect remains unknown. In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. The results of computational molecular docking analysis indicated that the interaction energy between cordycepin and NAD+‑dependent DNA ligase (LigA) was lower than that between cordycepin and other common antibacterial targets. The highly pure cordycepin obtained by prep‑HPLC demonstrated inhibitory activity against LigA from various bacteria in vitro. In conclusion, cordycepin may be useful as a broad‑spectrum antibiotic targeting LigA in various bacteria.
View Figures
View References

Related Articles

Journal Cover

September-2016
Volume 12 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhou X, Cai G, He Y and Tong G: Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor. Exp Ther Med 12: 1812-1816, 2016
APA
Zhou, X., Cai, G., He, Y., & Tong, G. (2016). Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor. Experimental and Therapeutic Medicine, 12, 1812-1816. https://doi.org/10.3892/etm.2016.3536
MLA
Zhou, X., Cai, G., He, Y., Tong, G."Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor". Experimental and Therapeutic Medicine 12.3 (2016): 1812-1816.
Chicago
Zhou, X., Cai, G., He, Y., Tong, G."Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1812-1816. https://doi.org/10.3892/etm.2016.3536