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MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway

  • Authors:
    • Guangyao Zhou
    • Wei Lin
    • Peipei Fang
    • Xiuzhen Lin
    • Lu Zhuge
    • Zhiqiu Hu
    • Lingxiang Jin
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Surgery, Minhang Hospital, Fudan University, Shanghai 201199, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1719-1722
    |
    Published online on: July 26, 2016
       https://doi.org/10.3892/etm.2016.3542
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Abstract

The aim of the present study was to examine the expression variation of the mouse hepatic fibrosis tissue transforming growth factor (TGF)-βl/Smads signal transduction pathway and its correlation with progression of hepatic fibrosis. The promotion effect of microRNA (miR)-10a on hepatic fibrosis and its possible mechanism was also assessed. Forty healthy female 8-week-old C57BL6/J mice were randomly divided into the control group (intraperitoneal injection of 5 µl/g normal saline, twice per week for 8 weeks) and the hepatic fibrosis group (intraperitoneal injection of 5 µl/g 10% CCI4 olive oil, twice per week for 8 weeks), with 20 mice per group. RT-PCR was used to test miR-10a expression in cells in the control and hepatic fibrosis groups. Cell culture and transfection of miR-10a mimics were conducted in the two groups and a Cell Counting Kit-8 was used to test the expression of TGF-β1 and Smad7 in hepatic fibroblasts. It was found that in comparison with the control group, miR-10a expression was significantly increased in the hepatic fibrosis group compared with the control group (P<0.05). The expression quantity of miR-10a was significantly increased in the transfection group compared with the control group (P<0.05). A high expression of miR-10a significantly improved TGF-β1 expression and reduced Smad7 expression in the hepatic fibrosis group (P<0.05). In conclusion, miR-10a expression was high in mouse hepatic tissues, transfection of miR-10a mimics significantly promoted the cell proliferation of hepatic fibrosis, and miR-10a improved hepatic fibrosis by regulating the TGF-βl/Smads signal transduction pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou G, Lin W, Fang P, Lin X, Zhuge L, Hu Z and Jin L: MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway. Exp Ther Med 12: 1719-1722, 2016.
APA
Zhou, G., Lin, W., Fang, P., Lin, X., Zhuge, L., Hu, Z., & Jin, L. (2016). MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway. Experimental and Therapeutic Medicine, 12, 1719-1722. https://doi.org/10.3892/etm.2016.3542
MLA
Zhou, G., Lin, W., Fang, P., Lin, X., Zhuge, L., Hu, Z., Jin, L."MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway". Experimental and Therapeutic Medicine 12.3 (2016): 1719-1722.
Chicago
Zhou, G., Lin, W., Fang, P., Lin, X., Zhuge, L., Hu, Z., Jin, L."MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1719-1722. https://doi.org/10.3892/etm.2016.3542
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou G, Lin W, Fang P, Lin X, Zhuge L, Hu Z and Jin L: MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway. Exp Ther Med 12: 1719-1722, 2016.
APA
Zhou, G., Lin, W., Fang, P., Lin, X., Zhuge, L., Hu, Z., & Jin, L. (2016). MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway. Experimental and Therapeutic Medicine, 12, 1719-1722. https://doi.org/10.3892/etm.2016.3542
MLA
Zhou, G., Lin, W., Fang, P., Lin, X., Zhuge, L., Hu, Z., Jin, L."MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway". Experimental and Therapeutic Medicine 12.3 (2016): 1719-1722.
Chicago
Zhou, G., Lin, W., Fang, P., Lin, X., Zhuge, L., Hu, Z., Jin, L."MiR-10a improves hepatic fibrosis by regulating the TGFβl/Smads signal transduction pathway". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1719-1722. https://doi.org/10.3892/etm.2016.3542
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