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Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats

  • Authors:
    • Zheng Chen
    • Qiaoting Kuang
    • Xue-Jun Lao
    • Jie Yang
    • Weidong Huang
    • Dong Zhou
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Xiangyang Hospital Affiliated to Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1775-1779
    |
    Published online on: July 26, 2016
       https://doi.org/10.3892/etm.2016.3543
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Abstract

The aim of the study was to investigate the possibility of human umbilical cord mesenchymal stem cells (UC-MSCs) surviving and differentiating into hepatocyte-like cells in partially hepatectomized model rats. MSCs were isolated from human umbilical cord and cultured with collagenase digestion. Cell surface markers were detected and fifth generation UC-MSCs were labeled with PKH26. The partially hepatectomized model rats were injected with the labeled human umbilical cord MSCs and transplanted through the portal vein. The survival of the labeled cells, in differentiation conditions and the expression of hepatic marker albumin were observed at post‑transplantation 1, 2 and 3 weeks under a fluorescence microscope. It was found that the human umbilical cord MSCs could be cultured and amplified in vitro. Following transplantation to the partially hepatectomized liver of the model rat, the cells survived and expresses the hepatic marker albumin in vivo. After being labeled with PKH26, the cells were visualized as red fluorescence under a fluorescence microscope. In the frozen sections of the liver, the marked cells scattered around and most of them expressed albumin with green fluorescence under the fluorescence microscope. In conclusion, the transplanted human umbilical cord MSCs survived and differentiated into hepatocyte-like cells. The human umbilical cord MSCs may therefore be a main source of hepatocytes in transplantation.
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1 

Mezey E and Chandross KJ: Bone marrow: a possible altern ative source of cells in the adult nervous system. Eur J Pharmacol. 4:297–302. 2000. View Article : Google Scholar

2 

Fukuda K and Preck D: Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes. C R Biol. 325:1027–1038. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Woodbury D, Schwarz EJ, Prockop DJ and Black IB: Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res. 61:364–370. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Yu SC, Xu YY, Li Y, Xu B, Sun Q, Li F and Zhang XG: Construction of tissue engineered skin with human amniotic mesenchymal stem cells and human amniotic epithelial cells. Eur Rev Med Pharmacol Sci. 19:4627–4635. 2015.PubMed/NCBI

5 

He J, Cai Y, Luo LM and Liu HB: Hypoxic adipose mesenchymal stem cells derived conditioned medium protects myocardial infarct in rat. Eur Rev Med Pharmacol Sci. 19:4397–4406. 2015.PubMed/NCBI

6 

Forraz N and McGuckin CP: The umbilical cord: a rich and ethical stem cell source to advance regenerative medicine. Cell Prolif. 44(Suppl 1): 60–69. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Lu LL, Song YP, Wei XD, Fang BJ, Zhang YL and Li YF: Comparative characterization of mesenchymal stem cells from human umbilical cord tissue and bone marrow. J Exp Hematol. 16:140–146. 2008.(In Chinese).

8 

Zhan YT, Wang Y, Wei L, Liu B, Chen HS, Cong X and Fei R: Differentiation of rat bone marrow stem cells in liver after partial hepatectomy. World J Gastroenterol. 12:5051–5054. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Blute JW, Douglas T, Witwer B, Zhang SC, Strable E, Lewis BK, et al: Magnetodendrimers allow endosomal magnetic labeling the in vivo tracking of stem cells. Nat Biotechnol. 19:1141–1147. 2011. View Article : Google Scholar

10 

Ji KH, Xiong J, Fan LX, Meng HK and Liu HQ: Rat marrow derived multipotent adult progenitor cells differentiate into skin epidermal cells in vivo. J Dermatol. 36:403–409. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Wallace PK and Muirhead KA: Cells tracking 2007: a proliferation of probes and applications. Immunol Invest. 36:527–561. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Haas J, Bauer P, Rolf A and Wree A: Immunocytochemical characterization PKH26 labelled an intracerebrally transplanted neonatal cells. Acta Histochem. 102:273–280. 2011. View Article : Google Scholar

13 

Fox D, Kouris GJ, Blumofe KA, Heilizer TJ, Husak V and Greisler HP: Optimizing fluorescent labeling of endothelial cells for tracking during long term studies of autologous transplantation. J Surg Res. 86:9–16. 2015. View Article : Google Scholar

14 

Weiss ML and Troyer DL: Stem cells in the umbilical cord. Stem Cell Rev. 2:155–162. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Li B, Tian XB, Hu RY, Xu FB and Zhao JM: Mechanism of BMP and TG2 in mesenchymal stem cell osteogenesis. Eur Rev Med Pharmacol Sci. 19:4214–4219. 2015.PubMed/NCBI

16 

Xue Z, Niu LY, An G, Guo YS, Lv SC and Ren XP: Expression of recombinant BMP-7 gene increased ossification activity in the rabbit bone mesenchymal stem cells. Eur Rev Med Pharmacol Sci. 19:3056–3062. 2015.PubMed/NCBI

17 

Hendrikx PJ, Martens CM, Hagenbeek A, Keij JF and Visser JW: Homing of fluorescently labeled murine hematopoietic stem cells. Exp Hematol. 24:129–140. 1996.PubMed/NCBI

18 

Oyagi S, Hirose M, Kojima M, Okuyama M, Kawase M, Nakamura T, Ohgushi H and Yagi K: Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CCl4-injured rats. J Hepatol. 44:742–748. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Zhang Y, Tang CL, Chen WJ, Zhang Q and Wang SL: Dynamic compression combined with exogenous SOX-9 promotes chondrogenesis of adipose-derived mesenchymal stem cells in PLGA scaffold. Eur Rev Med Pharmacol Sci. 19:2671–2678. 2015.PubMed/NCBI

20 

Han YF, Sun TJ, Han YQ, Xu G, Liu J and Tao R: Clinical perspectives on mesenchymal stem cells promoting wound healing in diabetes mellitus patients by inducing autophagy. Eur Rev Med Pharmacol Sci. 19:2666–2670. 2015.PubMed/NCBI

21 

Javazon EH, Beggs KJ and Flake AW: Mesenchymal stem cells: paradoxes of passaging. Exp Hematol. 32:414–425. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Wang J, Lu Y, He DM and Zhang Y: Isolation, purification and identification of mesenchymal stem cells derived from human umbilical cord. J Jinan University. 17:367–372. 2009.(In Chinese).

23 

Jia Z: Basic therapy and clinic of hepatopathy cells. Beijing: People's Medical Publishing House; pp. 139–140. 2005

24 

Ma J, Duan FL, Yan FG, Li WX, Wang X, Chen XY, Gao TH, Zhu WL and Wang ZQ: Serum from partial hepatectomy rat and hepatocyte growth factor stimulate bone marrow cell expressing albumin and alpha fetoprotein. Zhonghua Gan Zang Bing Za Zhi. 12:410–413. 2004.(In Chinese). PubMed/NCBI

25 

Li JW and Wu X: Mesenchymal stem cells ameliorate LPS-induced acute lung injury through KGF promoting alveolar fluid clearance of alveolar type II cells. Eur Rev Med Pharmacol Sci. 19:2368–2378. 2015.PubMed/NCBI

26 

Zhao YF, Luo YM, Xiong W, Ding W, Li YR, Zhao W, Zeng HZ, Gao HC and Wu XL: Mesenchymal stem cell-based FGF2 gene therapy for acute lung injury induced by lipopolysaccharide in mice. Eur Rev Med Pharmacol Sci. 19:857–865. 2015.PubMed/NCBI

27 

Garcea G and Maddern GJ: Liver failure after major hepatic resection. J Hepatobiliary Pancreat Surg. 16:145–155. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Sun TJ, Tao R, Han YQ, Xu G, Liu J and Han YF: Wnt3a promotes human umbilical cord mesenchymal stem cells to differentiate into epidermal-like cells. Eur Rev Med Pharmacol Sci. 19:86–91. 2015.PubMed/NCBI

29 

Roseti L, Serra M, Canella F, Munno C, Tosi A, Zuntini M, Pandolfi M, Sangiorgi L, Biso P, Pittalis MC, et al: In vitro gene and chromosome characterization of expanded bone marrow mesenchymal stem cells for musculo-skeletal applications. Eur Rev Med Pharmacol Sci. 18:3702–3711. 2014.PubMed/NCBI

30 

Xu Y, Sun DC, Wei ZT, Hong BF and Yang Y: Experimental study on transplantation of autologous minced muscle with human umbilical cord mesenchymal stem cells for urethral reconstruction. Eur Rev Med Pharmacol Sci. 18:3412–3419. 2014.PubMed/NCBI

31 

Yao XL, Li L, He XL, Cui L, Kuang W and Tang M: Activation of β-catenin stimulated by mechanical strain and estrogen requires estrogen receptor in mesenchymal stem cells (MSCs). Eur Rev Med Pharmacol Sci. 18:3149–3155. 2014.PubMed/NCBI

32 

Zhu XW, Zuo JL, Liu YH, Zang R, Li YK, Wang X and Li JM: Osteogenesis of umbilical mesenchymal stem cells is enhanced in absence of DNA methyltransferase 3B (DNMT3B) through upregulating Runx2 expression. Eur Rev Med Pharmacol Sci. 18:3004–3009. 2014.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen Z, Kuang Q, Lao X, Yang J, Huang W and Zhou D: Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats. Exp Ther Med 12: 1775-1779, 2016.
APA
Chen, Z., Kuang, Q., Lao, X., Yang, J., Huang, W., & Zhou, D. (2016). Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats. Experimental and Therapeutic Medicine, 12, 1775-1779. https://doi.org/10.3892/etm.2016.3543
MLA
Chen, Z., Kuang, Q., Lao, X., Yang, J., Huang, W., Zhou, D."Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats". Experimental and Therapeutic Medicine 12.3 (2016): 1775-1779.
Chicago
Chen, Z., Kuang, Q., Lao, X., Yang, J., Huang, W., Zhou, D."Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1775-1779. https://doi.org/10.3892/etm.2016.3543
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Kuang Q, Lao X, Yang J, Huang W and Zhou D: Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats. Exp Ther Med 12: 1775-1779, 2016.
APA
Chen, Z., Kuang, Q., Lao, X., Yang, J., Huang, W., & Zhou, D. (2016). Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats. Experimental and Therapeutic Medicine, 12, 1775-1779. https://doi.org/10.3892/etm.2016.3543
MLA
Chen, Z., Kuang, Q., Lao, X., Yang, J., Huang, W., Zhou, D."Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats". Experimental and Therapeutic Medicine 12.3 (2016): 1775-1779.
Chicago
Chen, Z., Kuang, Q., Lao, X., Yang, J., Huang, W., Zhou, D."Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1775-1779. https://doi.org/10.3892/etm.2016.3543
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