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Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3

  • Authors:
    • Ju‑Dong Lee
    • Haiyoung Jung
    • Sang‑Hyun Min
  • View Affiliations / Copyright

    Affiliations: Graduate School of Medical Science and Engineering, Biomedical Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305‑701, Republic of Korea, Immunotherapy Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305‑806, Republic of Korea, New Drug Development Center, Daegu‑Gyeongbuk Medical Innovation Foundation, Daegu 701‑310, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2974-2982
    |
    Published online on: September 20, 2016
       https://doi.org/10.3892/etm.2016.3722
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Abstract

The phosphatase of regenerating liver (PRL) family, including PRL-1, PRL-2, and PRL-3, comprises protein tyrosine phosphatases whose deregulation is associated with the tumorigenesis and metastasis of many types of cancer. However, the underlying mechanism is poorly understood. In this study, aiming to increase understanding of the molecular mechanisms underlying the functions of PRL‑1 and PRL‑3, a yeast two‑hybrid system was employed to screen for their interacting proteins. Alignment with the NCBI BLAST database revealed 12 interactive proteins: Synaptic nuclear envelope protein 2, emerin, mannose 6‑phosphate receptor‑binding protein 1, low‑density lipoprotein receptor‑related protein 10, Rab acceptor 1, tumor protein D52‑like 2, selectin P ligand (SELPLG), guanylate binding protein 1, transmembrane and ubiquitin‑like domain‑containing 2, NADH:ubiquinone oxidoreductase subunit B8, syndecan 4 and FK506‑binding protein 8 (FKBP8). These proteins are associated with cell proliferation, apoptosis, immune response, cell fate specification and metabolic process in biological process categories, and involved in various signaling pathways, including Alzheimer's disease, Parkinson's disease, Huntington's disease, hypertrophic cardiomyopathy and cell adhesion molecules. Interactions of PRL‑1 with the prey proteins SELPLG and FKBP8 were confirmed by immunoprecipitation or immunostaining. Furthermore, SELPLG and FKBP8 suppressed PRL‑1‑ or PRL‑3‑mediated p53 activity. Identification of the proteins interacting with PRL family proteins may provide valuable information to better understand the mechanism of PRL‑mediated signal transduction in cancer and other diverse diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Lee JD, Jung H and Min SH: Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3. Exp Ther Med 12: 2974-2982, 2016.
APA
Lee, J., Jung, H., & Min, S. (2016). Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3. Experimental and Therapeutic Medicine, 12, 2974-2982. https://doi.org/10.3892/etm.2016.3722
MLA
Lee, J., Jung, H., Min, S."Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3". Experimental and Therapeutic Medicine 12.5 (2016): 2974-2982.
Chicago
Lee, J., Jung, H., Min, S."Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3". Experimental and Therapeutic Medicine 12, no. 5 (2016): 2974-2982. https://doi.org/10.3892/etm.2016.3722
Copy and paste a formatted citation
x
Spandidos Publications style
Lee JD, Jung H and Min SH: Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3. Exp Ther Med 12: 2974-2982, 2016.
APA
Lee, J., Jung, H., & Min, S. (2016). Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3. Experimental and Therapeutic Medicine, 12, 2974-2982. https://doi.org/10.3892/etm.2016.3722
MLA
Lee, J., Jung, H., Min, S."Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3". Experimental and Therapeutic Medicine 12.5 (2016): 2974-2982.
Chicago
Lee, J., Jung, H., Min, S."Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3". Experimental and Therapeutic Medicine 12, no. 5 (2016): 2974-2982. https://doi.org/10.3892/etm.2016.3722
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