Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis

  • Authors:
    • Xin‑Cai Zhou
    • Yu‑Lan Li
    • De‑Xi Liu
    • Ying‑Guang Cao
    • Xin‑Pei Lu
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Baoan Maternal and Child Health Hospital, College of Medicine, Jinan University, Shenzhen, Guangdong 518133, P.R. China, Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, College of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P.R. China
  • Pages: 3073-3077
    |
    Published online on: September 20, 2016
       https://doi.org/10.3892/etm.2016.3726
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to assess the antimicrobial activity of plasma jet with helium (He) flowing through 3% hydrogen peroxide in root canals infected with Enterococcus faecalis. A total of 42 single‑rooted anterior teeth were prepared, sterilized, inoculated with an E. faecalis suspension and incubated for 7 days. Next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without He for different time durations) and one control group treated without plasma. The number of surviving bacteria in each canal was determined by counting the colony forming units (CFU)/ml on nutrient agar plates. The results indicated that statistically significant reduction in CFU/ml (P<0.005) existed for all treatment groups relative to the control group. The greatest reductions in CFU/ml were observed for Group 3 (7.027 log unit reduction) and Group 2 (6.237 log unit reduction), which were treated by plasma jet sterilization with He flowing through 3% hydrogen peroxide for 4 min or for 2 min, respectively. In addition, the reduction in Group 3 was significantly greater compared with that in Group 2 or in the groups treated by plasma jet sterilization without He flowing through 3% hydrogen peroxide for 1, 2 or 4 min. In conclusion, plasma jet with or without He flowing through 3% hydrogen peroxide can effectively sterilized root canals infected with E. faecalis and should be considered as an alternative method for root canal disinfection in endodontic treatments.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kakehashi S, Stanley HR and Fitzgerald RJ: The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol. 20:340–349. 1965. View Article : Google Scholar : PubMed/NCBI

2 

Möller AJ, Fabricius L, Dahlén G, Ohman AE and Heyden G: Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 89:475–484. 1981.PubMed/NCBI

3 

Fabricius L, Dahlén G, Sundqvist G, Happonen RP and Möller AJ: Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci. 114:278–285. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Walter J: Ecological role of lactobacilli in the gastrointestinal tract: Implications for fundamental and biomedical research. Appl Environ Microbiol. 74:4985–4996. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Wisplinghoff H, Seifert H, Tallent SM, Bischoff T, Wenzel RP and Edmond MB: Nosocomial bloodstream infections in pediatric patients in United States hospitals: Epidemiology, clinical features and susceptibilities. Pediatr Infect Dis J. 22:686–691. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Hancock HH III, Sigurdsson A, Trope M and Moiseiwitsch J: Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 91:579–586. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Evans M, Davies JK, Sundqvist G and Figdor D: Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 35:221–228. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Gordon S, Swenson JM, Hill BC, Pigott NE, Facklam RR, Cooksey RC, Thornsberry C, Jarvis WR and Tenover FC: Antimicrobial susceptibility patterns of common and unusual species of enterococci causing infections in the United States. Enterococcal Study Group. J Clin Microbiol. 30:2373–2378. 1992.PubMed/NCBI

9 

Haapasalo M and Orstavik D: In vitro infection and disinfection of dentinal tubules. J Dent Res. 66:1375–1379. 1987. View Article : Google Scholar : PubMed/NCBI

10 

Siqueira JF Jr, Rôças IN, Favieri A and Lima KC: Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod. 26:331–334. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Nair PN, Henry S, Cano V and Vera J: Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after ‘one-visit’ endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 99:231–252. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Sjögren U, Figdor D, Persson S and Sundqvist G: Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 30:297–306. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Sladek REJ, Stoffels E, Walraven R, Tielbeek PJA and Koolhoven RA: Plasma treatment of dental cavities: A feasibility study. IEEE Trans Plasma Sci. 32:1540–1543. 2004. View Article : Google Scholar

14 

Sladek REJ and Stoffels E: Deactivation of Escherichia coli by the plasma needle. J Phys D: Appl Phys. 38:1717–1721. 2005. View Article : Google Scholar

15 

Stoffels E, Kieft IE and Sladek REJ: Superficial treatment of mammalian cells using plasma needle. J Phys D: Appl Phys. 36:2908–2913. 2003. View Article : Google Scholar

16 

Fridman G, Friedman G, Gutsol A, Shekhter AB, Vasilets VN and Fridman A: Applied plasma medicine. Plasma Process Polym. 5:503–533. 2008. View Article : Google Scholar

17 

Sun P, Pan J, Tian Y, Bai N, Wu H, Wang L, Yu C, Zhang J, Zhu W, Becker K and Fang J: Tooth whitening with hydrogen peroxide assisted by a direct-current cold atmospheric-pressure air plasma microjet. IEEE Trans Plasma Sci. 38:1892–1896. 2010. View Article : Google Scholar

18 

Lee HW, Nam SH, Mohamed AAH, Kim GC and Lee JK: Atmospheric pressure plasma jet composed of three electrodes: Application to tooth bleaching. Plasma Process Polym. 7:274–280. 2010. View Article : Google Scholar

19 

Zhou X, Xiong Z, Cao Y, Lu X and Liu D: The antimicrobial activity of an atmospheric-pressure room-temperature plasma in a simulated root-canal model infected with Enterococcus faecalis. IEEE Trans Plasma Sci. 38:3370–3374. 2010. View Article : Google Scholar

20 

Cao Y, Yang P, Lu X, Xiong Z, Ye T, Xiong Q and Sun Z: Efficacy of atmospheric pressure plasma as an antibacterial agent against Enterococcus faecalis in vitro. Plasma Sci Tech. 13:932011. View Article : Google Scholar

21 

Chen W, Huang J, Du N, Liu XD, Wang XQ, Lv GH, Zhang GP, Guo LH and Yang SZ: Treatment of Enterococcus faecalis bacteria by a helium atmospheric cold plasma brush with oxygen addition. J Appl Phys. 112:0133042012. View Article : Google Scholar

22 

Lu X, Xiong Z, Zhao F, Xiam Y, Xiong Q, Gong W, Zhou C, Jiang Z and Pan Y: A simple atmospheric pressure room-temperature air plasma needle device for biomedical applications. Appl Phys Lett. 95:1815012009. View Article : Google Scholar

23 

Lu X, Cao Y, Yang P, Xiong Q, Xiong Z, Xian Y and Pan Y: An RC plasma device for sterilization of root canal of teeth. IEEE Trans Plasma Sci. 37:668–673. 2009. View Article : Google Scholar

24 

Distel JW, Hatton JF and Gillespie MJ: Biofilm formation in medicated root canals. J Endod. 28:689–693. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Kim GJ, Kim W, Kim KT and Lee JK: DNA damage and mitochondria dysfunction in cell apoptosis induced by nonthermal air plasma. Appl Phys Lett. 96:0215022010. View Article : Google Scholar

26 

Buttke TM and Sandstrom PA: Oxidative stress as a mediator of apoptosis. Immunol Today. 15:7–10. 1994. View Article : Google Scholar : PubMed/NCBI

27 

Simon HU, Haj-Yehia A and Levi-Schaffer F: Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis. 5:415–418. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Heaselgrave W, Andrew PW and Kilvington S: Acidified nitrite enhances hydrogen peroxide disinfection of Acanthamoeba, bacteria and fungi. J Antimicrob Chemother. 65:1207–1214. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Soloshenko IA, Tsiolko VV, Khomich VA, Bazhenov VY, Ryabtsev A, Schedrin AI and Mikhno IL: Features of sterilization using low-pressure DC-discharge hydrogen-peroxide plasma. IEEE Trans Plasma Sci. 30:1440–1444. 2002. View Article : Google Scholar

30 

Locke BR and Shih KY: Review of the methods to form hydrogen peroxide in electrical discharge plasma with liquid water. Plasma Sources Sci Technol. 20:34006–34020, (34015). 2011. View Article : Google Scholar

31 

Wang D, Zhao D, Feng K, Zhang X, Liu D and Yang S: The cold and atmospheric-pressure air surface barrier discharge plasma for large-area sterilization applications. Appl Phys Lett. 98:1615012011. View Article : Google Scholar

32 

Lu X, Ye T, Cao Y, Sun Z, Xiong Q, Tang Z, Xiong Z, Hu J, Jiang Z and Pan Y: The roles of the various plasma agents in the inactivation of bacteria. J Appl Phys. 104:0533092008. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou XC, Li YL, Liu DX, Cao YG and Lu XP: Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis. Exp Ther Med 12: 3073-3077, 2016.
APA
Zhou, X., Li, Y., Liu, D., Cao, Y., & Lu, X. (2016). Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis. Experimental and Therapeutic Medicine, 12, 3073-3077. https://doi.org/10.3892/etm.2016.3726
MLA
Zhou, X., Li, Y., Liu, D., Cao, Y., Lu, X."Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis". Experimental and Therapeutic Medicine 12.5 (2016): 3073-3077.
Chicago
Zhou, X., Li, Y., Liu, D., Cao, Y., Lu, X."Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3073-3077. https://doi.org/10.3892/etm.2016.3726
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou XC, Li YL, Liu DX, Cao YG and Lu XP: Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis. Exp Ther Med 12: 3073-3077, 2016.
APA
Zhou, X., Li, Y., Liu, D., Cao, Y., & Lu, X. (2016). Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis. Experimental and Therapeutic Medicine, 12, 3073-3077. https://doi.org/10.3892/etm.2016.3726
MLA
Zhou, X., Li, Y., Liu, D., Cao, Y., Lu, X."Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis". Experimental and Therapeutic Medicine 12.5 (2016): 3073-3077.
Chicago
Zhou, X., Li, Y., Liu, D., Cao, Y., Lu, X."Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against Enterococcus faecalis". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3073-3077. https://doi.org/10.3892/etm.2016.3726
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team