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Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation

  • Authors:
    • Bo Zhang
    • Qiu‑Hua Liu
    • Cui‑Jie Zhou
    • Ming‑Zheng Hu
    • Hai‑Xin Qian
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Zhangjiagang Hospital Affiliated to Soochow University, Zhangjiagang, Jiangsu 215600, P.R. China, Institute of Hepatopancreatobiliary Surgery, Yichang Central People's Hospital, China Three Gorges University, Yichang, Hubei 443003, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3181-3188
    |
    Published online on: September 30, 2016
       https://doi.org/10.3892/etm.2016.3762
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Abstract

Ischemia/reperfusion (I/R) injury can occur during small-for-size liver transplantation, resulting in delayed graft function and decreased long‑term graft survival. The aim of the present study was to evaluate the effects of genetic overexpression of endothelial nitric oxide synthase (eNOS) in protecting hepatocytes against I/R injury in a rat model of small-for-size liver transplantation. L02 liver cells were transfected with the eNOS gene using an adenovirus (Ad‑eNOS). eNOS expression was detected using quantitative polymerase chain reaction and western blot analysis. To evaluate the effect of eNOS overexpression, L02 cells were placed in a hypoxic environment for 12 h and immediately transferred to an oxygen‑enriched atmosphere. For in vivo testing, rats pretreated with Ad‑eNOS or control underwent small‑for‑size liver transplantation. At 6 h after reperfusion, the bile quantity, serum transaminase and nitric oxide (NO) levels, and histological outcomes were evaluated. Cell apoptosis was assessed by flow cytometry or TUNEL assay. In vitro, Ad‑eNOS prevented apoptosis in L02 cells with an increase in the level of NO in culture supernatant. In vivo, Ad‑eNOS pre‑treatment significantly increased bile production, improved abnormal transaminase levels, diminished apoptosis among liver cells, and decreased hepatocellular damage at 6 h after I/R injury. The eNOS‑mediated renal protective effects might be associated with the downregulation of tumor necrosis factor‑α and a reduction in macrophage activation in the early stage of reperfusion in small‑for‑size liver allografts. eNOS‑derived NO production significantly attenuates hepatic I/R injury. Thus, eNOS overexpression constitutes a promising therapeutic approach to prevent liver I/R injury following small‑for‑size liver transplantation.
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View References

1 

Li KK and Neuberger J: The management of patients awaiting liver transplantation. Nat Rev Gastroenterol Hepatol. 6:648–659. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Ma T, Liu H, Chen W, Xia X, Bai X, Liang L, Zhang Y and Liang T: Implanted adipose-derived stem cells attenuate small-for-size liver graft injury by secretion of VEGF in rats. Am J Transplant. 12:620–629. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Park GC, Song GW, Moon DB and Lee SG: A review of current status of living donor liver transplantation. Hepatobiliary Surg Nutr. 5:107–117. 2016.PubMed/NCBI

4 

Wang HS, Ohkohchi N, Enomoto Y, Usuda M, Miyagi S, Asakura T, Masuoka H, Aiso T, Fukushima K, Narita T, et al: Excessive portal flow causes graft failure in extremely small-for-size liver transplantation in pigs. World J Gastroenterol. 11:6954–6959. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Yamada N, Sanada Y, Hirata Y, Okada N, Ihara Y, Sasanuma H, Urahashi T, Sakuma Y, Yasuda Y and Mizuta K: The outcomes of pediatric living donor liver transplantation using small-for-size grafts: Experience of a single institute. Pediatr Surg Int. 32:363–368. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Wang W, Du Z, Yan J, Ma D, Shi M, Zhang M, Peng C and Li H: Mesenchymal stem cells promote liver regeneration and prolong survival in small-for-size liver grafts: Involvement of C-Jun N-terminal kinase, cyclin D1 and NF-κB. PLoS One. 9:e1125322014. View Article : Google Scholar : PubMed/NCBI

7 

Carini R and Albano E: Recent insights on the mechanisms of liver preconditioning. Gastroenterology. 125:1480–1491. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Weigand K, Brost S, Steinebrunner N, Büchler M, Schemmer P and Müller M: Ischemia/reperfusion injury in liver surgery and transplantation: pathophysiology. HPB Surg. 2012:1767232012. View Article : Google Scholar : PubMed/NCBI

9 

Kalogeris T, Baines CP, Krenz M and Korthuis RJ: Cell biology of ischemia/reperfusion injury. Int Rev Cell Mol Biol. 298:229–317. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Choukér A, Martignoni A, Schauer RJ, Dugas M, Schachtner T, Kaufmann I, Setzer F, Rau HG, Löhe F, Jauch KW, et al: Alpha-gluthathione S-transferase as an early marker of hepatic ischemia/reperfusion injury after liver resection. World J Surg. 29:528–534. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Selzner N, Rudiger H, Graf R and Clavien PA: Protective strategies against ischemic injury of the liver. Gastroenterology. 125:917–936. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Iñiguez M, Dotor J, Feijoo E, Goñi S, Prieto J, Berasain C and Avila MA: Novel pharmacologic strategies to protect the liver from ischemia-reperfusion injury. Recent Pat Cardiovasc Drug Discov. 3:9–18. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Han KH, Jung JY, Chung KY, Kim H and Kim J: Nitric oxide synthesis in the adult and developing kidney. Electrolyte Blood Press. 4:1–7. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Lundberg JO and Weitzberg E: NO-synthase independent NO generation in mammals. Biochem Biophys Res Commun. 396:39–45. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Mukhopadhyay P, Rajesh M, Horváth B, Bátkai S, Park O, Tanchian G, Gao RY, Patel V, Wink DA, Liaudet L, et al: Cannabidiol protects against hepatic ischemia/reperfusion injury by attenuating inflammatory signaling and response, oxidative/nitrative stress, and cell death. Free Radic Biol Med. 50:1368–1381. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Siriussawakul A, Zaky A and Lang JD: Role of nitric oxide in hepatic ischemia-reperfusion injury. World J Gastroenterol. 16:6079–6086. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Kamada N and Calne RY: A surgical experience with five hundred thirty liver transplants in the rat. Surgery. 93:64–69. 1983.PubMed/NCBI

18 

Tan F, Chen Z, Zhao Y, Liang T, Li J and Wei J: Novel technique for suprahepatic vena cava reconstruction in rat orthotopic liver transplantation. Microsurgery. 25:556–560. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Gueler F, Gwinner W, Schwarz A and Haller H: Long-term effects of acute ischemia and reperfusion injury. Kidney Int. 66:523–527. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Shimamura T, Zhu Y, Zhang S, Jin MB, Ishizaki N, Urakami A, Totsuka E, Kishida A, Lee R, Subbotin V, et al: Protective role of nitric oxide in ischemia and reperfusion injury of the liver. J Am Coll Surg. 188:43–52. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Grossini E, Pollesello P, Bellofatto K, Sigaudo L, Farruggio S, Origlia V, Mombello C, Mary DA, Valente G and Vacca G: Protective effects elicited by levosimendan against liver ischemia/reperfusion injury in anesthetized rats. Liver Transpl. 20:361–375. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Phillips L, Toledo AH, Lopez-Neblina F, Anaya-Prado R and Toledo-Pereyra LH: Nitric oxide mechanism of protection in ischemia and reperfusion injury. J Invest Surg. 22:46–55. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Lang JD Jr, Teng X, Chumley P, Crawford JH, Isbell TS, Chacko BK, Liu Y, Jhala N, Crowe DR, Smith AB, et al: Inhaled NO accelerates restoration of liver function in adults following orthotopic liver transplantation. J Clin Invest. 117:2583–2591. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Aiba M, Takeyoshi I, Ohwada S, Kawashima Y, Iwanami K, Sunose Y, Yamada T, Tsutsumi H, Matsumoto K and Morishita Y: Novel nitric oxide donor (FK409) ameliorates liver damage during extended liver resection with warm ischemia in dogs. J Am Coll Surg. 193:264–271. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Li SQ and Liang LJ: Protective mechanism of L-arginine against liver ischemic-reperfusion injury in rats. Hepatobiliary Pancreat Dis Int. 2:549–552. 2003.PubMed/NCBI

26 

Trocha M, Merwid-Lad A, Szuba A, Chlebda E, Pieśniewska M, Sozański T and Szelag A: Effect of simvastatin on nitric oxide synthases (eNOS, iNOS) and arginine and its derivatives (ADMA, SDMA) in ischemia/reperfusion injury in rat liver. Pharmacol Rep. 62:343–351. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Jones SP, Greer JJ, Kakkar AK, Ware PD, Turnage RH, Hicks M, van Haperen R, de Crom R, Kawashima S, Yokoyama M and Lefer DJ: Endothelial nitric oxide synthase overexpression attenuates myocardial reperfusion injury. Am J Physiol Heart Circ Physiol. 286:H276–H282. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Ghavami S, Hashemi M, Kadkhoda K, Alavian SM, Bay GH and Los M: Apoptosis in liver diseases - detection and therapeutic applications. Med Sci Monit. 11:RA337–RA345. 2005.PubMed/NCBI

29 

Duranski MR, Elrod JW, Calvert JW, Bryan NS, Feelisch M and Lefer DJ: Genetic overexpression of eNOS attenuates hepatic ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 291:H2980–H2986. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Abu-Amara M, Yang SY, Quaglia A, Rowley P, Fuller B, Seifalian A and Davidson B: Role of endothelial nitric oxide synthase in remote ischemic preconditioning of the mouse liver. Liver Transpl. 17:610–619. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Theruvath TP, Zhong Z, Currin RT, Ramshesh VK and Lemasters JJ: Endothelial nitric oxide synthase protects transplanted mouse livers against storage/reperfusion injury: Role of vasodilatory and innate immunity pathways. Transplant Proc. 38:3351–3357. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Palanisamy AP, Cheng G, Sutter AG, Liu J, Lewin DN, Chao J and Chavin K: Adenovirus-mediated eNOS expression augments liver injury after ischemia/reperfusion in mice. PLoS One. 9:e933042014. View Article : Google Scholar : PubMed/NCBI

33 

Mendes-Braz M, Elias-Miró M, Jimenez-Castro MB, Casillas-Ramírez A, Ramalho FS and Peralta C: The current state of knowledge of hepatic ischemia-reperfusion injury based on its study in experimental models. J Biomed Biotechnol. 2012:2986572012. View Article : Google Scholar : PubMed/NCBI

34 

Liang TB, Man K, Kin-Wah Lee T, Hong-Teng Tsui S, Lo CM, Xu X, Zheng SS, Fan ST and Wong J: Distinct intragraft response pattern in relation to graft size in liver transplantation. Transplantation. 75:673–678. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Perry BC, Soltys D, Toledo AH and Toledo-Pereyra LH: Tumor necrosis factor-α in liver ischemia/reperfusion injury. J Invest Surg. 24:178–188. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Perry BC, Soltys D, Toledo AH and Toledo-Pereyra LH: Tumor necrosis factor-α in liver ischemia/reperfusion injury. J Invest Surg. 24:178–188. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Zhang A, Chi X, Luo G, Hei Z, Xia H, Luo C, Wang Y, Mao X and Xia Z: Mast cell stabilization alleviates acute lung injury after orthotopic autologous liver transplantation in rats by downregulating inflammation. PLoS One. 8:e752622013. View Article : Google Scholar : PubMed/NCBI

38 

Czigány Z, Turóczi Z, Ónody P, Harsányi L, Lotz G, Hegedüs V and Szijártó A: Remote ischemic perconditioning protects the liver from ischemia-reperfusion injury. J Surg Res. 185:605–613. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Yang ZF, Ho DW, Chu AC, Wang YQ and Fan ST: Linking inflammation to acute rejection in small-for-size liver allografts: The potential role of early macrophage activation. Am J Transplant. 4:196–209. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Liu QH, Zhou CJ, Hu MZ and Qian HX: Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation. Exp Ther Med 12: 3181-3188, 2016.
APA
Zhang, B., Liu, Q., Zhou, C., Hu, M., & Qian, H. (2016). Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation. Experimental and Therapeutic Medicine, 12, 3181-3188. https://doi.org/10.3892/etm.2016.3762
MLA
Zhang, B., Liu, Q., Zhou, C., Hu, M., Qian, H."Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation". Experimental and Therapeutic Medicine 12.5 (2016): 3181-3188.
Chicago
Zhang, B., Liu, Q., Zhou, C., Hu, M., Qian, H."Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3181-3188. https://doi.org/10.3892/etm.2016.3762
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Liu QH, Zhou CJ, Hu MZ and Qian HX: Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation. Exp Ther Med 12: 3181-3188, 2016.
APA
Zhang, B., Liu, Q., Zhou, C., Hu, M., & Qian, H. (2016). Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation. Experimental and Therapeutic Medicine, 12, 3181-3188. https://doi.org/10.3892/etm.2016.3762
MLA
Zhang, B., Liu, Q., Zhou, C., Hu, M., Qian, H."Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation". Experimental and Therapeutic Medicine 12.5 (2016): 3181-3188.
Chicago
Zhang, B., Liu, Q., Zhou, C., Hu, M., Qian, H."Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3181-3188. https://doi.org/10.3892/etm.2016.3762
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