Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats

  • Authors:
    • Jian Yang
    • Zhixing Fan
    • Jun Yang
    • Jiawang Ding
    • Chaojun Yang
    • Lihua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Institute of Cardiovascular Diseases, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei 443000, P.R. China, Department of Optometry and Ophthalmology, Yichang Central People's Hospital, China Three Gorges University, Yichang, Hubei 443000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3249-3255
    |
    Published online on: October 4, 2016
       https://doi.org/10.3892/etm.2016.3777
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Previous studies have reported that microRNA-22 (miR-22) may be implicated in ischemia-reperfusion (I/R)-induced myocardial injury. Our previously published data also demonstrated that miR‑22 may protect against myocardial I/R injury via anti‑apoptosis in rats by targeting cAMP response element‑binding protein binding protein (CBP). However, the specific function of miR‑22 in myocardial I/R injury is far from fully elucidated. The present study was designed to investigate another cardioprotective signaling mechanism of miR‑22 in myocardial I/R injury. A total of 40 adult male Sprague‑Dawley rats were randomly divided into four equal groups (n=10): Sham, myocardial I/R, myocardial I/R with adenovirus expressing scramble miRNA (Ad‑Scramble) and myocardial I/R with adenovirus expressing miR‑22 (Ad‑miR‑22) groups. Besides the Sham operation group, the remaining three groups were artificially afflicted with coronary occlusion for 30 min and subsequently reperfused for 4 h. A light microscope was used to observe structural changes in the myocardium; reverse transcription polymerase chain reaction was used to measure the miR‑22 mRNA expression level; the myocardial infarct size was analyzed by the Evans Blue/triphenyltetrazolium chloride double‑staining; and p38 mitogen‑activated protein kinase (MAPK), CBP, c‑Jun‑activator protein (AP)‑1 and phospho (p)‑c‑Jun‑AP‑1 expression protein levels were detected by a western blot. Furthermore, ELISA was used to measure the levels of TNF‑α and IL‑6 in the myocardium. The results demonstrated that adenovirus‑mediated miR‑22 overexpression markedly reduced p38 MAPK, CBP, c‑Jun‑AP‑1, p‑c‑Jun‑AP‑1 expression levels concomitant with an improvement in myocardial injury, including smaller infarct size, reduced release of creatine kinase, lactate dehydrogenase and proinflammation mediators (tumor necrosis factor‑α and interleukin‑6). These findings suggest that miR‑22 has a protective effect on myocardial I/R injury. This protection mechanism, at least in part, is due to its anti‑inflammatory function via the suppression of the p38 MAPK/CBP/c‑Jun‑AP‑1 signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Ndrepepa G: Improving myocardial injury, infarct size, and myocardial salvage in the era of primary PCI for STEMI. Coron Artery Dis. 26:341–355. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Kloner RA: Does reperfusion injury exist in humans? J Am Coll Cardiol. 21:537–545. 1993. View Article : Google Scholar : PubMed/NCBI

3 

Eltzschig HK and Eckle T: Ischemia and reperfusion - from mechanism to translation. Nat Med. 17:1391–1401. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Hu H, Zhai C, Qian G, Gu A, Liu J, Ying F, Xu W, Jin D, Wang H, Hu H, Zhang Y and Tang G: Protective effects of tanshinone IIA on myocardial ischemia reperfusion injury by reducing oxidative stress, HMGB1 expression and inflammatory reaction. Pharm Biol. 53:1752–1758. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Du X, Hu X and Wei J: Anti-inflammatory effect of exendin-4 postconditioning during myocardial ischemia and reperfusion. Mol Biol Rep. 41:3853–3857. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Doddakula KK, Neary PM, Wang JH, Sookhai S, O'Donnell A, Aherne T, Bouchier-Hayes DJ and Redmond HP: The antiendotoxin agent taurolidine potentially reduces ischemia/reperfusion injury through its metabolite taurine. Surgery. 148:567–572. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Fan ZX and Yang J: Microribonucleic acids and vascular restenosis. Saudi Med J. 35:796–801. 2014.PubMed/NCBI

8 

Suzuki HI and Miyazono K: Emerging complexity of microRNA generation cascades. J Biochem. 149:15–25. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Peterson SM, Thompson JA, Ufkin ML, Sathyanarayana P, Liaw L and Congdon CB: Common features of microRNA target prediction tools. Front Genet. 5:232014. View Article : Google Scholar : PubMed/NCBI

10 

Thum T and Condorelli G: Long noncoding RNAs and microRNAs in cardiovascular pathophysiology. Circ Res. 116:751–762. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Lorenzen JM, Batkai S and Thum T: Regulation of cardiac and renal ischemia-reperfusion injury by microRNAs. Free Radic Biol Med. 64:78–84. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Yang J, Chen L, Yang J, Ding J, Li S, Wu H, Zhang J, Fan Z, Dong W and Li X: MicroRNA-22 targeting CBP protects against myocardial ischemia-reperfusion injury through anti-apoptosis in rats. Mol Biol Rep. 41:555–561. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Liu X, Shah A, Gangwani MR, Silverstein PS, Fu M and Kumar A: HIV-1 Nef induces CCL5 production in astrocytes through p38-MAPK and PI3K/Akt pathway and utilizes NF-kB, CEBP and AP-1 transcription factors. Sci Rep. 4:44502014.PubMed/NCBI

14 

McManus KJ and Hendzel MJ: CBP, a transcriptional coactivator and acetyltransferase. Biochem Cell Biol. 79:253–266. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Sugden PH and Clerk A: ‘Stress-responsive’ mitogen-activated protein kinases (c-Jun N-terminal kinases and p38 mitogen-activated protein kinases) in the myocardium. Circ Res. 83:345–352. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Zenz R, Eferl R, Scheinecker C, Redlich K, Smolen J, Schonthaler HB, Kenner L, Tschachler E and Wagner EF: Activator protein 1 (Fos/Jun) functions in inflammatory bone and skin disease. Arthritis Res Ther. 10:2012008. View Article : Google Scholar : PubMed/NCBI

17 

Karin M: The regulation of AP-1 activity by mitogen-activated protein kinases. J Biol Chem. 270:16483–16486. 1995. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Ma L, Liu H, Xie Z, Yang S, Xu W, Hou J and Yu B: Ginsenoside Rb3 protects cardiomyocytes against ischemia-reperfusion injury via the inhibition of JNK-mediated NF-κB pathway: A mouse cardiomyocyte model. PLoS One. 9:e1036282014. View Article : Google Scholar : PubMed/NCBI

20 

Yang W, Shao J, Bai X and Zhang G: Expression of plasma microRNA-1/21/208a/499 in myocardial ischemic reperfusion injury. Cardiology. 130:237–241. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Kumphune S, Surinkaew S, Chattipakorn SC and Chattipakorn N: Inhibition of p38 MAPK activation protects cardiac mitochondria from ischemia/reperfusion injury. Pharm Biol. 53:1831–1841. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Surinkaew S, Kumphune S, Chattipakorn S and Chattipakorn N: Inhibition of p38 MAPK during ischemia, but not reperfusion, effectively attenuates fatal arrhythmia in ischemia/reperfusion heart. J Cardiovasc Pharmacol. 61:133–141. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Yang J, Jiang H, Chen SS, Chen J, Li WQ, Xu SK and Wang JC: Lentivirus-mediated RNAi targeting CREB binding protein attenuates neointimal formation and promotes re-endothelialization in balloon injured rat carotid artery. Cell Physiol Biochem. 26:441–448. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Ait-Si-Ali S, Ramirez S, Barre FX, Dkhissi F, Magnaghi-Jaulin L, Girault JA, Robin P, Knibiehler M, Pritchard LL, Ducommun B, Trouche D and Harel-Bellan A: Histone acetyltransferase activity of CBP is controlled by cycle-dependent kinases and oncoprotein E1A. Nature. 396:184–186. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine AS and Kelly K: Recruitment of p300/CBP in p53-dependent signal pathways. Cell. 89:1175–1184. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Kappelmann M, Bosserhoff A and Kuphal S: AP-1/c-Jun transcription factors: Regulation and function in malignant melanoma. Eur J Cell Biol. 93:76–81. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Karin M and Gallagher E: From JNK to pay dirt: Jun kinases, their biochemistry, physiology and clinical importance. IUBMB Life. 57:283–295. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang J, Fan Z, Yang J, Ding J, Yang C and Chen L: microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats. Exp Ther Med 12: 3249-3255, 2016.
APA
Yang, J., Fan, Z., Yang, J., Ding, J., Yang, C., & Chen, L. (2016). microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats. Experimental and Therapeutic Medicine, 12, 3249-3255. https://doi.org/10.3892/etm.2016.3777
MLA
Yang, J., Fan, Z., Yang, J., Ding, J., Yang, C., Chen, L."microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats". Experimental and Therapeutic Medicine 12.5 (2016): 3249-3255.
Chicago
Yang, J., Fan, Z., Yang, J., Ding, J., Yang, C., Chen, L."microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3249-3255. https://doi.org/10.3892/etm.2016.3777
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Fan Z, Yang J, Ding J, Yang C and Chen L: microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats. Exp Ther Med 12: 3249-3255, 2016.
APA
Yang, J., Fan, Z., Yang, J., Ding, J., Yang, C., & Chen, L. (2016). microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats. Experimental and Therapeutic Medicine, 12, 3249-3255. https://doi.org/10.3892/etm.2016.3777
MLA
Yang, J., Fan, Z., Yang, J., Ding, J., Yang, C., Chen, L."microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats". Experimental and Therapeutic Medicine 12.5 (2016): 3249-3255.
Chicago
Yang, J., Fan, Z., Yang, J., Ding, J., Yang, C., Chen, L."microRNA‑22 attenuates myocardial ischemia‑reperfusion injury via an anti‑inflammatory mechanism in rats". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3249-3255. https://doi.org/10.3892/etm.2016.3777
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team