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Article

Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis

  • Authors:
    • Theodoros Eleftheriadis
    • Georgios Pissas
    • Maria Sounidaki
    • Nikolaos Antoniadis
    • Georgia Antoniadi
    • Vassilios Liakopoulos
    • Ioannis Stefanidis
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, University of Thessaly Medical School, 41110 Larissa, Greece, Organ Transplant Unit, Hippokration General Hospital, Aristotle University of Thessaloniki Medical School, 54642 Thessaloniki, Greece
  • Pages: 3471-3475
    |
    Published online on: October 6, 2016
       https://doi.org/10.3892/etm.2016.3786
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Abstract

Under growth conditions, angiogenin is translocated into the nucleus, where it enhances ribosomal RNA transcription, facilitating increased protein synthesis and cellular proliferation. During stress conditions, angiogenin is sequestered in the cytoplasm, where it cleaves transfer RNA (tRNA) to produce tRNA‑derived, stress‑induced small RNAs (tiRNAs) that inhibit global protein synthesis, but increase the translation of anti‑apoptotic factors. In the present study, the role of angiogenin in the human alloreactive immune response was evaluated using mixed lymphocyte reactions (MLRs) and neamine, an inhibitor of angiogenin nuclear translocation. In MLRs, angiogenin production was significantly (P<0.001) increased compared with resting peripheral blood mononuclear cells. The addition of neamine had no effect on cell proliferation, but did significantly (P<0.001) increase expression of Bcl‑2‑associated X protein and protein levels of activated caspase‑3 in CD4+ T‑cells isolated from the MLRs, indicating that angiogenin reduces apoptosis. In conclusion, angiogenin is upregulated during the alloreactive immune response, in which it does not affect the T‑cell expansion phase, but inhibits the T‑cell contraction phase by protecting against CD4+ T‑cell apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Eleftheriadis T, Pissas G, Sounidaki M, Antoniadis N, Antoniadi G, Liakopoulos V and Stefanidis I: Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis. Exp Ther Med 12: 3471-3475, 2016.
APA
Eleftheriadis, T., Pissas, G., Sounidaki, M., Antoniadis, N., Antoniadi, G., Liakopoulos, V., & Stefanidis, I. (2016). Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis. Experimental and Therapeutic Medicine, 12, 3471-3475. https://doi.org/10.3892/etm.2016.3786
MLA
Eleftheriadis, T., Pissas, G., Sounidaki, M., Antoniadis, N., Antoniadi, G., Liakopoulos, V., Stefanidis, I."Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis". Experimental and Therapeutic Medicine 12.5 (2016): 3471-3475.
Chicago
Eleftheriadis, T., Pissas, G., Sounidaki, M., Antoniadis, N., Antoniadi, G., Liakopoulos, V., Stefanidis, I."Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3471-3475. https://doi.org/10.3892/etm.2016.3786
Copy and paste a formatted citation
x
Spandidos Publications style
Eleftheriadis T, Pissas G, Sounidaki M, Antoniadis N, Antoniadi G, Liakopoulos V and Stefanidis I: Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis. Exp Ther Med 12: 3471-3475, 2016.
APA
Eleftheriadis, T., Pissas, G., Sounidaki, M., Antoniadis, N., Antoniadi, G., Liakopoulos, V., & Stefanidis, I. (2016). Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis. Experimental and Therapeutic Medicine, 12, 3471-3475. https://doi.org/10.3892/etm.2016.3786
MLA
Eleftheriadis, T., Pissas, G., Sounidaki, M., Antoniadis, N., Antoniadi, G., Liakopoulos, V., Stefanidis, I."Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis". Experimental and Therapeutic Medicine 12.5 (2016): 3471-3475.
Chicago
Eleftheriadis, T., Pissas, G., Sounidaki, M., Antoniadis, N., Antoniadi, G., Liakopoulos, V., Stefanidis, I."Angiogenin is upregulated during the alloreactive immune response and has no effect on the T‑cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T‑cell apoptosis". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3471-3475. https://doi.org/10.3892/etm.2016.3786
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