Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes

  • Authors:
    • Shiping Shi
    • Zhentao Man
    • Wei Li
    • Shui Sun
    • Wei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3161-3166
    |
    Published online on: October 11, 2016
       https://doi.org/10.3892/etm.2016.3788
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Osteoarthritis (OA) is a joint disease, and few treatments to date have been able to delay OA progression. The degradation of collagen type II (COL2) in the cartilage matrix is an important initiating factor for OA progression; the upregulation of Wnt5a protein activates COL2 degradation. In the present study, small interfering RNA of Wnt‑5a was delivered by a lentiviral vector (LV‑Wnt5a‑RNAi) to silence Wnt‑5a mRNA and prevent COL2 degradation. To determine the function of LV‑Wnt5a‑RNAi, the OA chondrocyte model (OA‑like chondrocytes) were constructed using interleukin (IL)‑1β. Detected using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), Wnt‑5a mRNA in the OA‑like chondrocytes were upregulated in a time‑dependent manner, indicating that OA‑like chondrocytes were successfully constructed. The bioactivity of OA‑like chondrocytes was determined using Live‑Dead staining, and the result illustrated that the OA‑like chondrocytes stimulated with IL‑1β for 6 h remained viable, and these were used in Wnt5a silencing. The OA‑like chondrocytes were divided into three groups: Group I, cultivated with common medium; group II, cultivated with common medium supplemented with empty lentiviral vector; group III, cultivated with common medium supplemented with LV‑Wnt5a‑RNAi. The efficiency of LV‑Wnt5a‑RNAi transfection was determined using fluorescence microscopy, the result of which indicated that LV‑Wnt5a‑RNAi could efficiently be transfected into the OA‑like chondrocytes. The LV‑Wnt5a‑RNAi efficiency for the Wnt5a mRNA silencing was determined using RT‑qPCR. The result illustrated that the mRNA of Wnt5a in group III was significantly lower in group I compared with that in group II (P<0.05), indicating that the LV‑Wnt5a‑RNAi could successfully silence Wnt5a mRNA. To further verify whether the silencing of Wnt5a mRNA could prevent COL2 degradation, western blotting and immunohistochemical analyses were performed. The results demonstrated that COL2 in group III was significantly higher compared with that in groups I and II (P<0.05), which illustrated that the silencing of Wnt5a mRNA could prevent COL2 degradation. In conclusion, LV‑Wnt5a‑RNAi was formed successfully and could efficiently silence Wnt5a mRNA expressed by OA‑like chondrocytes. In addition, the silencing of Wnt5a mRNA could prevent the degradation of COL2 in OA‑like chondrocytes, confirming that LV‑Wnt5a‑RNAi may be used as a novel tool for OA treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Ni GX, Li Z and Zhou YZ: The role of small leucine-rich proteoglycans in osteoarthritis pathogenesis. Osteoarthr Cartil. 22:896–903. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Hiligsmann M, Cooper C, Arden N, Boers M, Branco JC, Brandi M Luisa, Bruyère O, Guillemin F, Hochberg MC, Hunter DJ, et al: Health economics in the field of osteoarthritis: An expert's consensus paper from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO). Semin Arthritis Rheum. 43:303–313. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Cutolo M, Berenbaum F, Hochberg M, Punzi L and Reginster JY: Commentary on recent therapeutic guidelines for osteoarthritis. Semin Arthritis Rheum. 44:611–617. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Glyn-Jones S, Palmer AJ, Agricola R, Prince AJ, Vincent TL, Weinans H and Carr AJ: Osteoarthritis. Lancet. 386:376–387. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Evans CH and Huard J: Gene therapy approaches to regenerating the musculoskeletal system. Nat Rev Rheumatol. 11:234–242. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Bandara G, Robbins PD, Georgescu HI, Mueller GM, Glorioso JC and Evans CH: Gene transfer to synoviocytes: Prospects for gene treatment of arthritis. DNA Cell Biol. 11:227–231. 1992. View Article : Google Scholar : PubMed/NCBI

7 

Evans CH, Ghivizzani SC and Robbins PD: Getting arthritis gene therapy into the clinic. Nat Rev Rheumatol. 7:244–249. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Ivkovic A, Pascher A, Hudetz D, Maticic D, Jelic M, Dickinson S, Loparic M, Haspl M, Windhager R and Pecina M: Articular cartilage repair by genetically modified bone marrow aspirate in sheep. Gene Ther. 17:779–789. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Haupt JL, Frisbie DD, McIlwraith CW, Robbins PD, Ghvizzani S, Evans CH and Nixon AJ: Dual transduction of insulin-like growth factor-I and interleukin-1 receptor antagonist protein controls cartilage degradation in an osteoarthritic culture model. J Orth Res. 23:118–126. 2005. View Article : Google Scholar

10 

Shi Q, Zhang XL, Dai KR, Benderdour M and Fernandes JC: siRNA therapy for cancer and non-lethal diseases such as arthritis and osteoporosis. Expert Opin Biol Ther. 11:5–16. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Gao Y, Liu S, Huang J, Guo W, Chen J, Zhang L, Zhao B, Peng J, Wang A, Wang Y, et al: The ECM-cell interaction of cartilage extracellular matrix on chondrocytes. BioMed Res Int. 2014:6484592014. View Article : Google Scholar : PubMed/NCBI

12 

Tchetina EV, Squires G and Poole AR: Increased type II collagen degradation and very early focal cartilage degeneration is associated with upregulation of chondrocyte differentiation related genes in early human articular cartilage lesions. J Rheumatol. 32:876–886. 2005.PubMed/NCBI

13 

Li Z, Meng D, Li G, Xu J, Tian K and Li Y: Celecoxib combined with diacerein effectively alleviates osteoarthritis in rats via regulating JNK and p38MAPK signaling pathways. Inflammation. 38:1563–1572. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Mabey T and Honsawek S: Cytokines as biochemical markers for knee osteoarthritis. J Orthop. 6:95–105. 2015.

15 

Ge X, Ma X, Meng J, Zhang C, Ma K and Zhou C: Role of Wnt-5A in interleukin-1beta-induced matrix metalloproteinase expression in rabbit temporomandibular joint condylar chondrocytes. Arthritis Rheum. 60:2714–2722. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Ryu JH and Chun JS: Opposing roles of WNT-5A and WNT-11 in interleukin-1beta regulation of type II collagen expression in articular chondrocytes. J Biol Chem. 281:22039–22047. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Yu DG, Ding HF, Mao YQ, Liu M, Yu B, Zhao X, Wang XQ, Li Y, Liu GW, Nie SB, et al: Strontium ranelate reduces cartilage degeneration and subchondral bone remodeling in rat osteoarthritis model. Acta Pharmacol Sin. 34:393–402. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Wang PY and Tsai WB: Modulation of the proliferation and matrix synthesis of chondrocytes by dynamic compression on genipin-crosslinked chitosan/collagen scaffolds. J Biomater Sci Polym Ed. 24:507–519. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Yu L, Weng Y, Shui X, Fang W, Zhang E and Pan J: Multipotent Adult Progenitor Cells from Bone Marrow Differentiate into Chondrocyte-Like Cells. J Arthroplasty. 30:1273–1276. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Tekari A, Luginbuehl R, Hofstetter W and Egli RJ: Chondrocytes expressing intracellular collagen type II enter the cell cycle and co-express collagen type I in monolayer culture. J Orthop Res. 32:1503–1511. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Chen WP and Wu LD: Chlorogenic acid suppresses interleukin-1β-induced inflammatory mediators in human chondrocytes. Int J Clin Exp Pathol. 7:8797–8801. 2014.PubMed/NCBI

22 

Tao R, Wang S, Xia X, Wang Y, Cao Y, Huang Y, Xu X, Liu Z, Liu P, Tang X, et al: Pyrroloquinoline Quinone Slows Down the Progression of Osteoarthritis by Inhibiting Nitric Oxide Production and Metalloproteinase Synthesis. Inflammation. 38:1546–1555. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Xu HG, Zhang XH, Wang H, Liu P, Wang LT, Zuo CJ, Tong WX and Zhang XL: Intermittent Cyclic Mechanical Tension-Induced Calcification and downregulation of ankh gene expression of end plate chondrocytes. Spine. 37:1192–1197. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Lu J, Sun Y, Ge Q, Teng H and Jiang Q: Histone deacetylase 4 alters cartilage homeostasis in human osteoarthritis. BMC Musculoskelet Disord. 15:4382014. View Article : Google Scholar : PubMed/NCBI

26 

Wu PC, Tsai CL, Gordon GM, Jeong S, Itakura T, Patel N, Shi S and Fini ME: Chondrogenesis in scleral stem/progenitor cells and its association with form-deprived myopia in mice. Mol Vision. 21:138–147. 2015.

27 

Chen LX, Lin L, Wang HJ, Wei XL, Fu X, Zhang JY and Yu CL: Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-mediated NF-kappaBp65-specific siRNA. Osteoarthritis Cartilage. 16:174–184. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Hammond SM, Bernstein E, Beach D and Hannon GJ: An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature. 404:293–296. 2000. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shi S, Man Z, Li W, Sun S and Zhang W: Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes. Exp Ther Med 12: 3161-3166, 2016.
APA
Shi, S., Man, Z., Li, W., Sun, S., & Zhang, W. (2016). Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes. Experimental and Therapeutic Medicine, 12, 3161-3166. https://doi.org/10.3892/etm.2016.3788
MLA
Shi, S., Man, Z., Li, W., Sun, S., Zhang, W."Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes". Experimental and Therapeutic Medicine 12.5 (2016): 3161-3166.
Chicago
Shi, S., Man, Z., Li, W., Sun, S., Zhang, W."Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3161-3166. https://doi.org/10.3892/etm.2016.3788
Copy and paste a formatted citation
x
Spandidos Publications style
Shi S, Man Z, Li W, Sun S and Zhang W: Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes. Exp Ther Med 12: 3161-3166, 2016.
APA
Shi, S., Man, Z., Li, W., Sun, S., & Zhang, W. (2016). Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes. Experimental and Therapeutic Medicine, 12, 3161-3166. https://doi.org/10.3892/etm.2016.3788
MLA
Shi, S., Man, Z., Li, W., Sun, S., Zhang, W."Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes". Experimental and Therapeutic Medicine 12.5 (2016): 3161-3166.
Chicago
Shi, S., Man, Z., Li, W., Sun, S., Zhang, W."Silencing of Wnt5a prevents interleukin‑1β‑induced collagen type II degradation in rat chondrocytes". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3161-3166. https://doi.org/10.3892/etm.2016.3788
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team