Experimental studies on the inactivation of HBV in blood via riboflavin photochemical treatment
- Authors:
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Affiliations: Xuzhou Red Cross Blood Station, Xuzhou, Jiangsu 221006, P.R. China
- Published online on: November 22, 2016 https://doi.org/10.3892/etm.2016.3922
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Pages:
222-224
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Copyright: © Zhou
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
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Abstract
The aim of the study was to examine the influence of riboflavin photochemical method on the inactivation of hepatitis B virus (HBV) and the functions of red blood cells. Twenty patients suffering viral hepatitis B were selected in this study, and venous blood was collected and final concentration of 1,500 µmol/l riboflavin were added, to accept the λ=400-500 nm. The light intensity of 40,000 lux was treated with 2 h. The effect of inactivation was then evaluated and the function of red blood cells was detected. Two hours after treatment of the blood samples with riboflavin (1,500 µmol/l), the numbers of copy of HBV DNA were significantly decreased (5.1x109±4.2x10 vs. 1.2x107±1.2x106 after the inactivation, while, 2,3-DPG, Na+K+-ATPase, Ca2+‑ATPase, Mg2+-ATPase, FHb were unchanged. In conclusion, HBV DNA can be reduced using riboflavin photochemical inactivation method. Inactivate the B virus had no effect on erythrocyte function.
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