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Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin

  • Authors:
    • Baoxin Wang
    • Junjie Yuan
    • Jiafeng Xu
    • Xinwei Chen
    • Xinjiang Ying
    • Pin Dong
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Head and Neck Surgery, Shanghai Jiao Tong University Affiliated to Shanghai First People's Hospital, Shanghai 201620, P.R. China, Department of Orthopedics, Shanghai Fengxian District Central Hospital, Shanghai Jiao Tong University Affiliated to Sixth People's Hospital South Campus, Shanghai 200011, P.R. China, School of Economics and Finance, Shanghai International Studies University, Shanghai 200083, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 178-186
    |
    Published online on: November 23, 2016
       https://doi.org/10.3892/etm.2016.3925
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Abstract

Damage to the recurrent laryngeal nerve often causes hoarseness, dyspnea, dysphagia, and sometimes asphyxia due to vocal cord paralysis which result in a reduction of quality of life. Brain‑derived neurotrophic factor (BDNF) and glial cell line‑derived neurotrophic factor (GDNF) play critical roles in peripheral nerve regeneration. However, methods for efficiently delivering these molecules are lacking, which limits their use in clinical applications. The present study reports an effective strategy for targeting BDNF and GDNF to laminin by fusing the N‑terminal domains of these molecules with agrin (NtA). More specifically, laminin‑binding efficacy was assessed and sustained release assays of the delivery of BDNF or GDNF fused with NtA (LBD‑BDNF or LBD‑GDNF) to laminin were conducted in vitro. In addition, the bioactivity of LBD‑BDNF and LBD‑GDNF on laminin in vitro was investigated. LBD‑BDNF and LBD‑GDNF were each able to specifically bind to laminin and maintain their activity in vitro. Moreover, neurotrophic factors with NtA retained higher concentrations and bioactivity levels compared with those without NtA. The ratio of LBD‑BDNF and LBD‑GDNF that produced optimal effects was 4:6. BDNF and GDNF fused with NtA were effective in specifically binding to laminin. As laminin is a major component of the extracellular matrix, LBD‑BDNF and LBD‑GDNF may prove useful in the repair of peripheral nerve injuries.
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1 

Tessema B, Roark RM, Pitman MJ, Weissbrod P, Sharma S and Schaefer SD: Observations of recurrent laryngeal nerve injury and recovery using a rat model. Laryngoscope. 119:1644–1651. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Choi JS, Oh SH, An HY, Kim YM, Lee JH and Lim JY: Functional regeneration of recurrent laryngeal nerve injury during thyroid surgery using an asymmetrically porous nerve guide conduit in an animal model. Thyroid. 24:52–59. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Araki K, Shiotani A, Watabe K, Saito K, Moro K and Ogawa K: Adenoviral GDNF gene transfer enhances neurofunctional recovery after recurrent laryngeal nerve injury. Gene Ther. 13:296–303. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Navarro X, Vivó M and Valero-Cabré A: Neural plasticity after peripheral nerve injury and regeneration. Prog Neurobiol. 82:163–201. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Lundborg G: A 25-year perspective of peripheral nerve surgery: Evolving neuroscientific concepts and clinical significance. J Hand Surg Am. 25:391–414. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Woodson GE: Spontaneous laryngeal reinnervation after recurrent laryngeal or vagus nerve injury. Ann Otol Rhinol Laryngol. 116:57–65. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Guan J, Zhang B, Zhang J, Ding W, Xiao Z, Zhu Z, Han Q, Wu C, Sun Y, Tong W, et al: Nerve regeneration and functional recovery by collagen-binding brain-derived neurotrophic factor in an intracerebral hemorrhage model. Tissue Eng Part A. 21:62–74. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Henderson CE, Phillips HS, Pollock RA, Davies AM, Lemeulle C, Armanini M, Simmons L, Moffet B, Vandlen RA, Simpson LC; corrected to, ; Simmons L, et al: GDNF: A potent survival factor for motoneurons present in peripheral nerve and muscle. Science. 266:1062–1064. 1994. View Article : Google Scholar : PubMed/NCBI

9 

Li L, Wu W, Lin LF, Lei M, Oppenheim RW and Houenou LJ: Rescue of adult mouse motoneurons from injury-induced cell death by glial cell line-derived neurotrophic factor. Proc Natl Acad Sci USA. 92:9771–9775. 1995. View Article : Google Scholar : PubMed/NCBI

10 

Yan Q, Matheson C and Lopez OT: In vivo neurotrophic effects of GDNF on neonatal and adult facial motor neurons. Nature. 373:341–344. 1995. View Article : Google Scholar : PubMed/NCBI

11 

Sakamoto T, Watabe K, Ohashi T, Kawazoe Y, Oyanagi K, Inoue K and Eto Y: Adenoviral vector-mediated GDNF gene transfer prevents death of adult facial motoneurons. Neuroreport. 11:1857–1860. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Yanamoto H, Nagata I, Sakata M, Zhang Z, Tohnai N, Sakai H and Kikuchi H: Infarct tolerance induced by intra-cerebral infusion of recombinant brain-derived neurotrophic factor. Brain Res. 859:240–248. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Han Q, Li B, Feng H, Xiao Z, Chen B, Zhao Y, Huang J and Dai J: The promotion of cerebral ischemia recovery in rats by laminin-binding BDNF. Biomaterials. 32:5077–5085. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Longo FM, Hayman EG, Davis GE, Ruoslahti E, Engvall E, Manthorpe M and Varon S: Neurite-promoting factors and extracellular matrix components accumulating in vivo within nerve regeneration chambers. Brain Res. 309:105–117. 1984. View Article : Google Scholar : PubMed/NCBI

15 

Lander AD, Fujii DK and Reichardt LF: Purification of a factor that promotes neurite outgrowth: Isolation of laminin and associated molecules. J Cell Biol. 101:898–913. 1985. View Article : Google Scholar : PubMed/NCBI

16 

Martini R: Expression and functional roles of neural cell surface molecules and extracellular matrix components during development and regeneration of peripheral nerves. J Neurocytol. 23:1–28. 1994. View Article : Google Scholar : PubMed/NCBI

17 

Fu SY and Gordon T: The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol. 14:67–116. 1997. View Article : Google Scholar : PubMed/NCBI

18 

Mascarenhas JB, Rüegg MA, Winzen U, Halfter W, Engel J and Stetefeld J: Mapping of the laminin-binding site of the N-terminal agrin domain (NtA). EMBO J. 22:529–536. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Gu Z, Weidenhaupt M, Ivanova N, Pavlov M, Xu B, Su ZG and Janson JC: Chromatographic methods for the isolation of, and refolding of proteins from, Escherichia coli inclusion bodies. Protein Expr Purif. 25:174–179. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Sun W, Sun C, Zhao H, Lin H, Han Q, Wang J, Ma H, Chen B, Xiao Z and Dai J: Improvement of sciatic nerve regeneration using laminin-binding human NGF-beta. PLoS One. 4:e61802009. View Article : Google Scholar : PubMed/NCBI

21 

Yang XN, Jin YQ, Bi H, Wei W, Cheng J, Liu ZY, Shen Z, Qi ZL and Cao Y: Peripheral nerve repair with epimysium conduit. Biomaterials. 34:5606–5616. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Levi-Montalcini R: The nerve growth factor: Thirty-five years later. Biosci Rep. 7:681–699. 1987. View Article : Google Scholar : PubMed/NCBI

23 

Sun W, Lin H, Chen B, Zhao W, Zhao Y, Xiao Z and Dai J: Collagen scaffolds loaded with collagen-binding NGF-beta accelerate ulcer healing. J Biomed Mater Res A. 92:887–895. 2010.PubMed/NCBI

24 

Sun W, Lin H, Chen B, Zhao W, Zhao Y and Dai J: Promotion of peripheral nerve growth by collagen scaffolds loaded with collagen-targeting human nerve growth factor-beta. J Biomed Mater Res A. 83:1054–1061. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Sun W, Sun C, Lin H, Zhao H, Wang J, Ma H, Chen B, Xiao Z and Dai J: The effect of collagen-binding NGF-beta on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model. Biomaterials. 30:4649–4656. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Kitajima T, Terai H and Ito Y: A fusion protein of hepatocyte growth factor for immobilization to collagen. Biomaterials. 28:1989–1997. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Han Q, Sun W, Lin H, Zhao W, Gao Y, Zhao Y, Chen B, Xiao Z, Hu W, Li Y, et al: Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats. Tissue Eng Part A. 15:2927–2935. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Han Q, Jin W, Xiao Z, Ni H, Wang J, Kong J, Wu J, Liang W, Chen L, Zhao Y, et al: The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody. Biomaterials. 31:9212–9220. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Liang W, Han Q, Jin W, Xiao Z, Huang J, Ni H, Chen B, Kong J, Wu J and Dai J: The promotion of neurological recovery in the rat spinal cord crushed injury model by collagen-binding BDNF. Biomaterials. 31:8634–8641. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Guan J, Tong W, Ding W, Du S, Xiao Z, Han Q, Zhu Z, Bao X, Shi X, Wu C, et al: Neuronal regeneration and protection by collagen-binding BDNF in the rat middle cerebral artery occlusion model. Biomaterials. 33:1386–1395. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Han S, Wang B, Jin W, Xiao Z, Chen B, Xiao H, Ding W, Cao J, Ma F, Li X, et al: The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord. 52:867–873. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Han S, Wang B, Jin W, Xiao Z, Li X, Ding W, Kapur M, Chen B, Yuan B, Zhu T, et al: The linear-ordered collagen scaffold-BDNF complex significantly promotes functional recovery after completely transected spinal cord injury in canine. Biomaterials. 41:89–96. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Cao J, Sun C, Zhao H, Xiao Z, Chen B, Gao J, Zheng T, Wu W, Wu S, Wang J and Dai J: The use of laminin modified linear ordered collagen scaffolds loaded with laminin-binding ciliary neurotrophic factor for sciatic nerve regeneration in rats. Biomaterials. 32:3939–3948. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Moro K, Shiotani A, Watabe K, Takeda Y, Saito K, Mori Y and Ogawa K: Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Res. 1076:1–8. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Shiotani A, Saito K, Araki K, Moro K and Watabe K: Gene therapy for laryngeal paralysis. Ann Otol Rhinol Laryngol. 116:115–122. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Chen ZL and Strickland S: Laminin gamma 1 is critical for Schwann cell differentiation, axon myelination, and regeneration in the peripheral nerve. J Cell Biol. 163:889–899. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang B, Yuan J, Xu J, Chen X, Ying X and Dong P: Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin. Exp Ther Med 13: 178-186, 2017.
APA
Wang, B., Yuan, J., Xu, J., Chen, X., Ying, X., & Dong, P. (2017). Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin. Experimental and Therapeutic Medicine, 13, 178-186. https://doi.org/10.3892/etm.2016.3925
MLA
Wang, B., Yuan, J., Xu, J., Chen, X., Ying, X., Dong, P."Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin". Experimental and Therapeutic Medicine 13.1 (2017): 178-186.
Chicago
Wang, B., Yuan, J., Xu, J., Chen, X., Ying, X., Dong, P."Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin". Experimental and Therapeutic Medicine 13, no. 1 (2017): 178-186. https://doi.org/10.3892/etm.2016.3925
Copy and paste a formatted citation
x
Spandidos Publications style
Wang B, Yuan J, Xu J, Chen X, Ying X and Dong P: Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin. Exp Ther Med 13: 178-186, 2017.
APA
Wang, B., Yuan, J., Xu, J., Chen, X., Ying, X., & Dong, P. (2017). Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin. Experimental and Therapeutic Medicine, 13, 178-186. https://doi.org/10.3892/etm.2016.3925
MLA
Wang, B., Yuan, J., Xu, J., Chen, X., Ying, X., Dong, P."Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin". Experimental and Therapeutic Medicine 13.1 (2017): 178-186.
Chicago
Wang, B., Yuan, J., Xu, J., Chen, X., Ying, X., Dong, P."Brain‑derived and glial cell line‑derived neurotrophic factor fusion protein immobilization to laminin". Experimental and Therapeutic Medicine 13, no. 1 (2017): 178-186. https://doi.org/10.3892/etm.2016.3925
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