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Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis

  • Authors:
    • Liming Qing
    • Pengfei Lei
    • Hao Liu
    • Jie Xie
    • Long Wang
    • Ting Wen
    • Yihe Hu

  • View Affiliations

  • Published online on: December 1, 2016     https://doi.org/10.3892/etm.2016.3940
  • Pages: 63-68

  • Copyright: © Qing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine hypoxia-inducible factor 1α (HIF-1α) levels in the synovial fluid and articular cartilage of patients with primary knee osteoarthritis (OA) and to investigate their association with the severity of disease. A total of 36 patients with knee OA and ten healthy controls were enrolled. Anteroposterior knee radiographs and/or Mankin scores were assessed to determine the disease severity of the affected knee. Radiographic grading of OA in the knee was performed according to Kellgren-Lawrence criteria. HIF‑1α levels in synovial fluid were measured using enzyme‑linked immunosorbent assay, whereas HIF‑1α levels in articular cartilage were assessed with immunohistochemical methods. Compared with healthy controls, OA patients exhibited an increased HIF‑1α concentration in synovial fluid (218.17±25.12 vs. 156.66±7.74 pg/ml; P<0.001) and articular cartilage (P<0.05). Furthermore, synovial fluid HIF‑1α levels demonstrated a positive correlation with articular cartilage HIF‑1α levels (Pearson's P=0.815; P<0.001). Subsequent analysis showed that synovial fluid HIF‑1α levels were significantly correlated with the severity of disease (Spearman's ρ=0.933; P<0.001). Furthermore, articular cartilage levels of HIF‑1α also correlated with disease severity (Spearman's ρ=‑0.967; P<0.001). The findings of the present study suggested that HIF-1α in synovial fluid and articular cartilage is associated with progressive joint damage and is likely to be a useful biomarker for determining disease severity and progression in knee OA.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-0981
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Spandidos Publications style
Qing L, Lei P, Liu H, Xie J, Wang L, Wen T and Hu Y: Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis. Exp Ther Med 13: 63-68, 2017
APA
Qing, L., Lei, P., Liu, H., Xie, J., Wang, L., Wen, T., & Hu, Y. (2017). Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis. Experimental and Therapeutic Medicine, 13, 63-68. https://doi.org/10.3892/etm.2016.3940
MLA
Qing, L., Lei, P., Liu, H., Xie, J., Wang, L., Wen, T., Hu, Y."Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis". Experimental and Therapeutic Medicine 13.1 (2017): 63-68.
Chicago
Qing, L., Lei, P., Liu, H., Xie, J., Wang, L., Wen, T., Hu, Y."Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis". Experimental and Therapeutic Medicine 13, no. 1 (2017): 63-68. https://doi.org/10.3892/etm.2016.3940