Open Access

Ag85B synergizes with ESAT-6 to induce efficient and long-term immunity of C57BL/6 mice primed with recombinant Bacille Calmette-Guerin

  • Authors:
    • Wei Liu
    • Ying Xu
    • Jingran Yan
    • Hongbo Shen
    • Enzhuo Yang
    • Honghai Wang
  • View Affiliations

  • Published online on: December 1, 2016     https://doi.org/10.3892/etm.2016.3944
  • Pages: 208-214
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The latest probable scenario in vaccination strategies is to prime one live attenuated vaccine candidate followed by boost dose of second vaccine candidate. In the present study, we primed the mice with a recombinant Bacille Calmette-Guerin (BCG) comprising Ag85B and ESAT‑6 followed by boost doses of Ag85B, ESAT‑6 and Ag85B-ESAT-6 fusion protein in the DDA adjuvant, separately. After boost doses of 8 and 12 weeks, the levels of antigen‑stimulated T cells secreting interferon (IFN)‑γ, the content of the IFN‑γ, tumor necrosis factor‑α and interleukin‑4 in the splenocytes in vitro culture supernatant, the antigen‑specific immunoglobulin (Ig)G titer from mouse serum, IgG subclass and the population of antigen‑specific CD4+ and CD8+ T cells were detected. The present study showed that recombinant BCG along with boost doses of Ag85B or ESAT‑6 individually did not induce efficient T‑helper (Th) 1‑type immune response. On the other hand, recombinant BCG with boost doses of Ag85B‑ESAT‑6 fusion protein enhanced longer lasting predominant Th1 immune response. This result suggested that Ag85B might synergize with ESAT‑6 protein in order to produce better as well as effective immune response. Thus, the present study concluded recombinant BCG with boost doses of Ag85B-ESAT-6 fusion protein could be a good strategy to improve the immune protective efficacy.
View Figures
View References

Related Articles

Journal Cover

January-2017
Volume 13 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Xu Y, Yan J, Shen H, Yang E and Wang H: Ag85B synergizes with ESAT-6 to induce efficient and long-term immunity of C57BL/6 mice primed with recombinant Bacille Calmette-Guerin. Exp Ther Med 13: 208-214, 2017
APA
Liu, W., Xu, Y., Yan, J., Shen, H., Yang, E., & Wang, H. (2017). Ag85B synergizes with ESAT-6 to induce efficient and long-term immunity of C57BL/6 mice primed with recombinant Bacille Calmette-Guerin. Experimental and Therapeutic Medicine, 13, 208-214. https://doi.org/10.3892/etm.2016.3944
MLA
Liu, W., Xu, Y., Yan, J., Shen, H., Yang, E., Wang, H."Ag85B synergizes with ESAT-6 to induce efficient and long-term immunity of C57BL/6 mice primed with recombinant Bacille Calmette-Guerin". Experimental and Therapeutic Medicine 13.1 (2017): 208-214.
Chicago
Liu, W., Xu, Y., Yan, J., Shen, H., Yang, E., Wang, H."Ag85B synergizes with ESAT-6 to induce efficient and long-term immunity of C57BL/6 mice primed with recombinant Bacille Calmette-Guerin". Experimental and Therapeutic Medicine 13, no. 1 (2017): 208-214. https://doi.org/10.3892/etm.2016.3944