Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia Retraction in /10.3892/etm.2025.12831

Li,Junguo; Zhang,Li; Li,Bing

doi:10.3892/etm.2016.3959

January-2017 Volume 13 Issue 1

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January-2017 Volume 13 Issue 1

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Article Open Access

Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia

Retraction in: /10.3892/etm.2025.12831

Authors:
- Junguo Li
- Li Zhang
- Bing Li
View Affiliations / Copyright

Affiliations: Reproductive Medicine Center, General Hospital of Beijing Military Region, Beijing 100700, P.R. China, Department of Information, General Hospital of Beijing Military Region, Beijing 100700, P.R. China, Department of Obstetrics and Gynecology, General Hospital of Beijing Military Region, Beijing 100700, P.R. China

Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 127-130
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Published online on: December 6, 2016

https://doi.org/10.3892/etm.2016.3959
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Abstract

The aim of the present study was to investigate the possible mechanism of Janus kinase (JAK)-signal transduction and activator of transcription (STAT)/PSMβ3 signaling in the occurrence of asthenozoospermia. We examined seminal fluid samples from 30 cases of asthenozoospermia and 30 healthy controls. Sperm was collected using the Percoll density gradient centrifugation method. The expression of JAK, STAT and PSMβ3 mRNA was assessed by reverse-transcription quantitative PCR and the protein levels of p-JAK, p-STAT and PSMβ3 were measured by western blot analysis. The PSMβ3 mRNA and protein expression levels were also measured after application of a JAK inhibitor, AG-490, to the control group, with a FITC-labeled monoclonal rabbit anti‑human PSMβ3 primary antibody. The cells were observed under a laser confocal microscope. The mRNA levels of JAK, STAT and PSMβ3 in asthenozoospermia were decreased significantly (P<0.05). The protein levels of p-JAK, p-STAT and PSMβ3 in asthenozoospermia were also reduced and the differences were statistically significant (P<0.05). The PSMβ3 mRNA and protein expression levels were decreased in the control group after treatment with the JAK inhibitor, and levels were approximately equal to those of the asthenozoospermia group. PSMβ3 was mainly expressed in round-headed sperm, and less in asthenozoospermia. In conclusion, the JAK-STAT/PSMβ3 signaling transduction pathway may be involved in the pathogenic mechanism of asthenozoospermia.

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