Levodopa attenuates cellular apoptosis in steroid‑associated necrosis of the femoral head

Xi,Hongbo; Tao,Weijian; Jian,Zhengguang; Sun,Xuefeng; Gong,Xiaohong; Huang,Lixin; Dong,Tianhua

doi:10.3892/etm.2016.3964

January-2017 Volume 13 Issue 1

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January-2017 Volume 13 Issue 1

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Article Open Access

Levodopa attenuates cellular apoptosis in steroid‑associated necrosis of the femoral head

Authors:
- Hongbo Xi
- Weijian Tao
- Zhengguang Jian
- Xuefeng Sun
- Xiaohong Gong
- Lixin Huang
- Tianhua Dong
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Affiliations: Department of Orthopaedics, Xiangcheng People's Hospital, Suzhou, Jiangsu 215131, P.R. China, Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China

Copyright: © Xi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 69-74
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Published online on: December 7, 2016

https://doi.org/10.3892/etm.2016.3964
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Abstract

The present study aimed to investigate the effects of levodopa (LEV) on cellular apoptosis in a rabbit model of steroid‑associated necrosis of the femoral head (SANFH). A total of 44 healthy adult Chinese rabbits were randomly divided into three groups: Group A (n=15), administered a combination of lipopolysaccharide and hormone to establish the SANFH animal model; group B (n=15), SANFH animal model as in group A orally administered LEV (0.4 g/kg/day) on the day of injection; and group C (n=14), the control group. On the 6th and 8th week of modeling, seven rabbits from each group were sacrificed to harvest bilateral femoral head specimens for hematoxylin and eosin staining and apoptosis detection by terminal deoxynucleotidyl transferase dUTP nick‑end labeling assay analysis, as well as for observing pathological changes and analyzing cellular apoptosis. Eight weeks after modeling, the serum insulin‑like growth factor (IGF)‑1 levels of the three groups were measured. The empty lacunae rate and apoptosis index of bone cells in the treatment group were significantly lower than that of the model group (P<0.01). Eight weeks after treatment, the serum levels of IGF‑1 were significantly higher than that of the model group (P<0.01). These findings suggested that LEV was able to reduce steroid‑induced bone cellular apoptosis, reduce the occurrence of necrosis of the femoral head and, through in vivo metabolism, it may promote the synthesis and release of IGF‑1, which could be one of its biological pathways to prevent and treat SANFH.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Levodopa attenuates cellular apoptosis in steroid‑associated necrosis of the femoral head

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