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Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils

  • Authors:
    • Yuehui Liu
    • Xinhua Zhu
    • Hao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 215-221
    |
    Published online on: December 7, 2016
       https://doi.org/10.3892/etm.2016.3965
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Abstract

The present study aimed to use RNA interference (RNAi) to silence chemokine receptor 3 (CCR3) and observe the effects on eosinophils (EOS) in mice with allergic rhinitis (AR). CCR3 small interfering RNA (siRNA) lentiviral vectors were transduced into purified EOS cells cultured in vitro. Reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analyses were also used to detect the efficiency of silencing, and flow cytometry was used to detect the EOS apoptosis rates. Experimental mice were grouped for nasal administration, and the lentivirus was then dispensed to AR mice. RT‑PCR and western blots were performed to detect the expression levels of CCR3 mRNA and protein in EOS extracted from bone marrow, peripheral blood and nasal mucosa. Furthermore, flow cytometry was performed to detect changes to CD34‑positive (CD34+) cells in each group. The CCR3 siRNA lentiviral vector exhibited high efficiency in silencing CCR3 mRNA and protein expression, inhibited growth and promoted apoptosis of EOS. In addition, the expression of CCR3 mRNA and protein in the bone marrow, peripheral blood and nasal mucosa of mice in the CCR3 siRNA treatment group were lower than those in the control group (P<0.05), whereas the number of CD34+ cells in the CCR3 siRNA treatment group was not significantly different compared with that in the control group (P>0.05). CCR3 RNAi could effectively silence the expression of CCR3 mRNA and protein both in vitro and in vivo, thus promoting apoptosis of EOS and inhibiting its growth, migration and invasion.
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1 

Kim JH, Yoon MG, Seo DH, Kim BS, Ban GY, Ye YM, Shin YS and Park HS: Detection of Allergen specific antibodies from nasal secretion of allergic rhinitis patients. Allergy Asthma Immunol Res. 8:329–337. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Franzke N, Schäfer I, Jost K, Blome C, Rustenbach SJ, Reich K, Reusch M, Maurer M and Augustin M: A new instrument for the assessment of patient-defined benefit in the treatment of allergic rhinitis. Allergy. 66:665–670. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Fu CH, Tsai WC, Lee TJ, Huang CC, Chang PH and Su Pang JH: Simvastatin Inhibits IL-5-Induced Chemotaxis and CCR3 Expression of HL-60-Derived and Human Primary Eosinophils. PLoS One. 11:e01571862016. View Article : Google Scholar : PubMed/NCBI

4 

Pawankar R, Bunnag C, Khaltaev N and Bousquet J: Allergic rhinitis and its impact on asthma in asia pacific and the ARIA update 2008. World Allergy Organ J. 5:(Suppl 3). S212–S217. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Zhang Y and Zhang L: Prevalence of allergic rhinitis in China. Allergy Asthma Immunol Res. 6:105–113. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Han DH, Ahn JC, Mun SJ, Park SK, Oh SY and Rhee CS: Novel Risk Factors for Allergic Rhinitis in Korean Elementary School Children: ARCO-kids Phase II in a Community. Allergy Asthma Immunol Res. 7:234–240. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Hu X, Wang J, Xia Y, Simayi M, Ikramullah S, He Y, Cui S, Li S and Wushouer Q: Resveratrol induces cell cycle arrest and apoptosis in human eosinophils from asthmatic individuals. Mol Med Rep. 2016.

8 

Fulkerson PC and Rothenberg ME: Targeting eosinophils in allergy, inflammation and beyond. Nat Rev Drug Discov. 2:117–129. 2013. View Article : Google Scholar

9 

Muir AB, Markowitz JE and Liacouras CA: 45 – Allergic and Eosinophilic Gastrointestinal Disease. Pediatric Allergy Principles & Practice. 399–408. 2016. View Article : Google Scholar

10 

Khorasanizadeh M, Eskian M, Assa'ad AH, Camargo CA Jr and Rezaei N: Efficacy and Safety of Benralizumab, a Monoclonal Antibody against IL-5Rα, in Uncontrolled Eosinophilic Asthma. Int Rev Immunol. 35:294–311. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Mahajan L, Madan T, Sarma P Usha and Kishore U: Human lung surfactant protein, SP-D, modulates eosinophil activation and survival and enhances phagocytosis of apoptotic bosinophils. US patent, US8883730. 2014

12 

Cruz FF, Borg ZD, Goodwin M, Coffey AL, Wagner DE, Rocco PR and Weiss DJ: CD11b+ and Sca-1+ Cells Exert the Main Beneficial Effects of Systemically Administered Bone Marrow-Derived Mononuclear Cells in a Murine Model of Mixed Th2/Th17 Allergic Airway Inflammation. Stem Cells Transl Med. 5:488–499. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Long H, Liao W, Wang L and Lu Q: A Player and Coordinator: The Versatile Roles of Eosinophils in the Immune System. Transfus Med Hemother. 43:96–108. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Smith H, Whittall C, Weksler B and Middleton J: Chemokines stimulate bidirectional migration of human mesenchymal stem cells across bone marrow endothelial cells. Stem Cells Dev. 21:476–486. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Millard CJ, Ludeman JP, Canals M, Bridgford JL, Hinds MG, Clayton DJ, Christopoulos A, Payne RJ and Stone MJ: Structural basis of receptor sulfotyrosine recognition by a CC chemokine: The N-terminal region of CCR3 bound to CCL11/eotaxin-1. Structure. 22:1571–1581. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Rupprecht KM, Daugherty B, Mudgett J and Parsons WH: Chapter 14. CCR3 antagonists for the treatment of respiratory diseases. Annu Rep Med Chem. 38:131–140. 2003. View Article : Google Scholar

17 

Proudfoot AE: Chemokine receptors: Multifaceted therapeutic targets. Nat Rev Immunol. 2:106–115. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Lee JH, Jang AS, Park SW, Kim DJ and Park CS: Gene-Gene Interaction Between CCR3 and Eotaxin Genes: The Relationship With Blood Eosinophilia in Asthma. Allergy Asthma Immunol Res. 6:55–60. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Lee JH, Jang AS, Park SW, Kim DJ and Park CS: Gene-Gene Interaction Between CCR3 and Eotaxin Genes: The Relationship With Blood Eosinophilia in Asthma. Allergy Asthma Immunol Res. 6:55–60. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Rose CE Jr, Lannigan JA, Kim P, Lee JJ, Fu SM and Sung SS: Murine lung eosinophil activation and chemokine production in allergic airway inflammation. Cell Mol Immunol. 7:361–374. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Gauvreau GM and Denburg JA: Hemopoietic progenitors: The role of eosinophil/basophil progenitors in allergic airway inflammation. Expert Rev Clin Immunol. 1:87–101. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Rådinger M and Lötvall J: Eosinophil progenitors in allergy and asthma - do they matter? Pharmacol Ther. 121:174–184. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Waseda K, Hagiya H, Hanayama Y, Terasaka T, Kimura K, Tsuzuki T, Hasegawa K, Nada T, Nakamura E, Murakami K, et al: Complication of chronic eosinophilic pneumonia in an elderly patient with Sjögren syndrome. Acta Med Okayama. 69:123–127. 2015.PubMed/NCBI

24 

Sakai-Kashiwabara M, Abe S and Asano K: Suppressive activity of quercetin on the production of eosinophil chemoattractants from eosinophils in vitro. In Vivo. 28:515–522. 2014.PubMed/NCBI

25 

Ryu SH, Na HY, Sohn M, Han SM, Choi W, In H, Hong S, Jeon H, Seo JY, Ahn J, et al: Reduced expression of granule proteins during extended survival of eosinophils in splenocyte culture with GM-CSF. Immunol Lett. 173:7–20. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Burnham ME, Koziol-White CJ, Esnault S, Bates ME, Evans MD, Bertics PJ and Denlinger LC: Human airway eosinophils exhibit preferential reduction in STAT signaling capacity and increased CISH expression. J Immunol. 191:2900–2906. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Lioy DT and Klein M: Compositions and Methods for Regulating Gene Expression. US patent, US20090082297. 2009.

28 

Zhu XH, Liao B, Liu K and Liu YH: Effect of RNA interference therapy on the mice eosinophils CCR3 gene and granule protein in the murine model of allergic rhinitis. Asian Pac J Trop Med. 7:226–230. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Mohr SE, Smith JA, Shamu CE, Neumüller RA and Perrimon N: RNAi screening comes of age: Improved techniques and complementary approaches. Nat Rev Mol Cell Biol. 15:591–600. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Wong TW, Doyle AD, Lee JJ and Jelinek DF: Eosinophils regulate peripheral B cell numbers in both mice and humans. J Immunol. 192:3548–3558. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Chang K-C, Lo C-W, Fan TC, Chang MD, Shu CW, Chang CH, Chung CT, Fang SL, Chao CC, Tsai JJ, et al: TNF-α mediates eosinophil cationic protein-induced apoptosis in BEAS-2B cells. BMC Cell Biol. 11:62010. View Article : Google Scholar : PubMed/NCBI

32 

Curran CS: Human eosinophil adhesion and receptor expression. Methods Mol Biol. 1178:129–141. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Mehta P and Furuta GT: Eosinophils in Gastrointestinal Disorders: Eosinophilic Gastrointestinal Diseases, Celiac Disease, Inflammatory Bowel Diseases, and Parasitic Infections. Immunol Allergy Clin North Am. 35:413–437. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Bao L, Zhang H and Chan LS: The involvement of the JAK-STAT signaling pathway in chronic inflammatory skin disease atopic dermatitis. JAK-STAT. 2:e241372013. View Article : Google Scholar : PubMed/NCBI

35 

Forssmann U, Elsner J, Escher S and Sposberg N: Method of inhibiting the emigration of cells from the intravascular compartment into tissues. US patent, US7741292. 2010

36 

Zhu Y, Tuerxun A, Hui Y and Abliz P: Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro. Exp Ther Med. 8:647–651. 2014.PubMed/NCBI

37 

Ben S, Li X, Xu F, Xu W, Li W, Wu Z, Huang H, Shi H and Shen H: Treatment with anti-CC chemokine receptor 3 monoclonal antibody or dexamethasone inhibits the migration and differentiation of bone marrow CD34 progenitor cells in an allergic mouse model. Allergy. 63:1164–1176. 2008. View Article : Google Scholar : PubMed/NCBI

38 

Gelfand EW and Dakhama A: Method for reducing allergen-induced airway hyperresponsiveness. Patent, EP1265626. 2005

39 

Lee CC and Chiang BL: RNA interference: New therapeutics in allergic diseases. Curr Gene Ther. 8:236–246. 2008. View Article : Google Scholar : PubMed/NCBI

40 

Zhang Y, Cristofaro P, Silbermann R, Pusch O, Boden D, Konkin T, Hovanesian V, Monfils PR, Resnick M and Moss SF: Engineering mucosal RNA interference in vivo. Mol Ther. 14:336–342. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Zhu X and Zhang H: Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils. Exp Ther Med 13: 215-221, 2017.
APA
Liu, Y., Zhu, X., & Zhang, H. (2017). Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils. Experimental and Therapeutic Medicine, 13, 215-221. https://doi.org/10.3892/etm.2016.3965
MLA
Liu, Y., Zhu, X., Zhang, H."Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils". Experimental and Therapeutic Medicine 13.1 (2017): 215-221.
Chicago
Liu, Y., Zhu, X., Zhang, H."Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils". Experimental and Therapeutic Medicine 13, no. 1 (2017): 215-221. https://doi.org/10.3892/etm.2016.3965
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Zhu X and Zhang H: Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils. Exp Ther Med 13: 215-221, 2017.
APA
Liu, Y., Zhu, X., & Zhang, H. (2017). Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils. Experimental and Therapeutic Medicine, 13, 215-221. https://doi.org/10.3892/etm.2016.3965
MLA
Liu, Y., Zhu, X., Zhang, H."Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils". Experimental and Therapeutic Medicine 13.1 (2017): 215-221.
Chicago
Liu, Y., Zhu, X., Zhang, H."Effects of chemokine receptor 3 gene silencing by RNA interference on eosinophils". Experimental and Therapeutic Medicine 13, no. 1 (2017): 215-221. https://doi.org/10.3892/etm.2016.3965
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