Open Access

Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro

  • Authors:
    • Miao Chen
    • Li‑Hua Dai
    • Aihua Fei
    • Shu‑Ming Pan
    • Hai‑Rong Wang
  • View Affiliations

  • Published online on: January 31, 2017     https://doi.org/10.3892/etm.2017.4093
  • Pages: 1353-1359
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Isoquercetin has exhibited a wide range of therapeutic properties, including antioxidant, anti-inflammatory and anti‑allergic activities. The aim of the present study was to investigate the effect of isoquercetin on rats with 2 h middle cerebral artery occlusion (MCAO) and evaluate the neuroprotective effect of isoquercetin on a primary culture of rat hippocampal neuronal cells subjected to oxygen‑glucose deprivation followed by reoxygenation (OGD/R). In vivo, the rats treated with isoquercetin exhibited a lower degree of neurological dysfunction and smaller infarct volume than the vehicle‑treated rats. In vitro, it was found that isoquercetin prevented the OGD/R‑induced increase in apoptosis, lactate dehydrogenase release and reduction in cell viability. Additionally, isoquercetin induced the upregulation of nuclear factor erythroid 2-related factor 2 gene and protein expression, and increased extracellular signal‑regulated kinase 1 and 2 (ERK1/2) phosphorylation. This indicates that the ERK1/2 pathway may contribute to the neuroprotective effect of isoquercetin against OGD/R‑induced oxidative damage in rat hippocampal neurons. These findings suggest the potential importance of isoquercetin in the treatment of ischemia/reperfusion-related brain injury and associated diseases.
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April-2017
Volume 13 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen M, Dai LH, Fei A, Pan SM and Wang HR: Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro. Exp Ther Med 13: 1353-1359, 2017.
APA
Chen, M., Dai, L., Fei, A., Pan, S., & Wang, H. (2017). Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro. Experimental and Therapeutic Medicine, 13, 1353-1359. https://doi.org/10.3892/etm.2017.4093
MLA
Chen, M., Dai, L., Fei, A., Pan, S., Wang, H."Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro". Experimental and Therapeutic Medicine 13.4 (2017): 1353-1359.
Chicago
Chen, M., Dai, L., Fei, A., Pan, S., Wang, H."Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1353-1359. https://doi.org/10.3892/etm.2017.4093