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TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque

  • Authors:
    • Yijin Su
    • Changsong Xu
    • Kunyu Li
    • Bo Wang
    • Jufang Chen
    • Long Liu
    • Lizhou Lin
    • Qingqing Dong
    • Lianfang Du
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Shanghai General Hospital Affiliated to Nanjing Medical University, Shanghai 200080, P.R. China
    Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1465-1469
    |
    Published online on: February 6, 2017
       https://doi.org/10.3892/etm.2017.4101
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Abstract

In the present study, we determined whether ultrasound-targeted microbubble destruction (UTMD) combined with dual targeting of transforming growth factor (TGF)-β1 and tissue inhibitors of metalloproteinase (TIMP) 1 recombinant adeno-associated virus (rAAV) can stabilize atherosclerotic vulnerable plaques. First, we used rabbit model to detect the TGF-β1/TIMP1 virus therapy result. H&E staining was used to evaluate the tissues. The protein levels of TGF-β1 and TIMP1 were detected in rabbit models. The THP-1 cells were induced into macrophages, and transfected with TGF-β1 and TIMP1 rAAV under optimized UTMD. The expression of TGF-β1 and TIMP1 was measured by RT-PCR and western blotting. We found that the apoptotic rates were induced when compared to the control group. The rAAV virus group showed a significant decrease in the p-ERT and p-AKT expression. These data support the hypothesis that TGF-β1 and TIMP1 are crucial in the regulation of atherosclerotic plaques.
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Copy and paste a formatted citation
Spandidos Publications style
Su Y, Xu C, Li K, Wang B, Chen J, Liu L, Lin L, Dong Q and Du L: TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque. Exp Ther Med 13: 1465-1469, 2017.
APA
Su, Y., Xu, C., Li, K., Wang, B., Chen, J., Liu, L. ... Du, L. (2017). TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque. Experimental and Therapeutic Medicine, 13, 1465-1469. https://doi.org/10.3892/etm.2017.4101
MLA
Su, Y., Xu, C., Li, K., Wang, B., Chen, J., Liu, L., Lin, L., Dong, Q., Du, L."TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque". Experimental and Therapeutic Medicine 13.4 (2017): 1465-1469.
Chicago
Su, Y., Xu, C., Li, K., Wang, B., Chen, J., Liu, L., Lin, L., Dong, Q., Du, L."TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1465-1469. https://doi.org/10.3892/etm.2017.4101
Copy and paste a formatted citation
x
Spandidos Publications style
Su Y, Xu C, Li K, Wang B, Chen J, Liu L, Lin L, Dong Q and Du L: TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque. Exp Ther Med 13: 1465-1469, 2017.
APA
Su, Y., Xu, C., Li, K., Wang, B., Chen, J., Liu, L. ... Du, L. (2017). TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque. Experimental and Therapeutic Medicine, 13, 1465-1469. https://doi.org/10.3892/etm.2017.4101
MLA
Su, Y., Xu, C., Li, K., Wang, B., Chen, J., Liu, L., Lin, L., Dong, Q., Du, L."TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque". Experimental and Therapeutic Medicine 13.4 (2017): 1465-1469.
Chicago
Su, Y., Xu, C., Li, K., Wang, B., Chen, J., Liu, L., Lin, L., Dong, Q., Du, L."TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1465-1469. https://doi.org/10.3892/etm.2017.4101
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