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Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression

  • Authors:
    • Pin Guan
    • Wentao Cai
    • Huapeng Yu
    • Zhiyong Wu
    • Wei Li
    • Jie Wu
    • Juan Chen
    • Guangqiu Feng
  • View Affiliations / Copyright

    Affiliations: Graduate School, Southern Medical University, Guangzhou, Guangdong 510000, P.R. China, Department of Orthopedics, People's Hospital of Hainan, Haikou, Hainan 570311, P.R. China, Department of Respiratory Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510000, P.R. China, Department of Medical Center, People's Hospital of Hainan, Haikou, Hainan 570311, P.R. China, Institute of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 570311, P.R. China
    Copyright: © Guan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1408-1414
    |
    Published online on: February 15, 2017
       https://doi.org/10.3892/etm.2017.4126
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Abstract

Cigarette smoke has been considered a major contributor to the pathogenesis of chronic obstructive pulmonary disease (COPD). In COPD patients, the airway smooth muscle layer has been observed to be markedly thickened and the proliferation of airway smooth muscle cells (ASMCs) was therefore used by the present study as a model to assess the impact of cigarette smoke extract (CSE). ASMCs were exposed to various concentrations of CSE and the proliferation of the cells was analyzed by an MTT assay. Furthermore, the expression levels of calreticulin and CCAAT/enhancer‑binding protein alpha (C/EBP-α) in CSE-stimulated ASMCs were determined by polymerase chain reaction and western blot analyses. In addition, the effects of RNA interference (RNAi) to knockdown calreticulin and/or C/EBP‑α on ASMC proliferation were studied. CSE was found to promote the proliferation of ASMCs, which was associated with increased expression of calreticulin and decreased expression of C/EBP-α. Knockdown of calreticulin resulted in the upregulation of C/EBP‑α and inhibition of cell proliferation, while simultaneous knockdown of C/EBP‑α promoted cell proliferation. The present study revealed that CSE promoted the proliferation of ASMCs, which was mediated by inhibition of C/EBP-α. These findings shed new light on airway remodeling in COPD and may provide novel approaches for therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Guan P, Cai W, Yu H, Wu Z, Li W, Wu J, Chen J and Feng G: Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression. Exp Ther Med 13: 1408-1414, 2017.
APA
Guan, P., Cai, W., Yu, H., Wu, Z., Li, W., Wu, J. ... Feng, G. (2017). Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression. Experimental and Therapeutic Medicine, 13, 1408-1414. https://doi.org/10.3892/etm.2017.4126
MLA
Guan, P., Cai, W., Yu, H., Wu, Z., Li, W., Wu, J., Chen, J., Feng, G."Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression". Experimental and Therapeutic Medicine 13.4 (2017): 1408-1414.
Chicago
Guan, P., Cai, W., Yu, H., Wu, Z., Li, W., Wu, J., Chen, J., Feng, G."Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1408-1414. https://doi.org/10.3892/etm.2017.4126
Copy and paste a formatted citation
x
Spandidos Publications style
Guan P, Cai W, Yu H, Wu Z, Li W, Wu J, Chen J and Feng G: Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression. Exp Ther Med 13: 1408-1414, 2017.
APA
Guan, P., Cai, W., Yu, H., Wu, Z., Li, W., Wu, J. ... Feng, G. (2017). Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression. Experimental and Therapeutic Medicine, 13, 1408-1414. https://doi.org/10.3892/etm.2017.4126
MLA
Guan, P., Cai, W., Yu, H., Wu, Z., Li, W., Wu, J., Chen, J., Feng, G."Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression". Experimental and Therapeutic Medicine 13.4 (2017): 1408-1414.
Chicago
Guan, P., Cai, W., Yu, H., Wu, Z., Li, W., Wu, J., Chen, J., Feng, G."Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-α expression". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1408-1414. https://doi.org/10.3892/etm.2017.4126
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