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Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB

  • Authors:
    • Haizhou Xu
    • Bing Mei
    • Meitang Wang
    • Shuogui Xu
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1398-1402
    |
    Published online on: February 16, 2017
       https://doi.org/10.3892/etm.2017.4130
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Abstract

To investigate the effect of inhibitor κBα (IκBα) on severe pneumonia and explain the mechanisms of nuclear factor κB (NF‑κB), the activation of NF‑κB was induced in Sprague-Dawley (SD) rats infected with Klebsiella pneumoniae (K. pneumoniae). The rats were then treated with differing concentrations of IκBα protein. A histological analysis was performed to compare the lung structure prior to and following treatment, and an immunohistochemistry assay was used to detect NF‑κB activity. In addition, the expression of certain inflammatory factors was detected using a protein chip assay. The severe pneumonia rat model was successfully produced and in model rats, NF‑κB was activated by K. pneumoniae. Following treatment with IκBα, the activity of NF‑κB was inhibited and pneumonia symptoms in model rats were alleviated. Furthermore, the expression of a number of inflammatory factors including tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), interferon γ (IFN‑γ) and monocyte chemoattractant protein‑1 (MCP‑1) were also inhibited. The current study demonstrates that NF‑κB inhibition with IκBα protein therapy prevents the development of pneumonia in a K. pneumoniae rat model. The therapeutic effect is indicated by the responses of proinflammatory factors, including TNF-α, IL-6, IFN-γ and MCP-1.
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1 

Mathers CD, Boerma T and Ma Fat DM: Global and regional causes of death. Br Med Bull. 92:7–32. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Sahly H and Podschun R: Clinical, bacteriological, and serological aspects of Klebsiella infections and their spondylarthropathic sequelae. Clin Diagn Lab Immunol. 4:393–399. 1997.PubMed/NCBI

3 

Lin CY, Wheelock AM, Morin D, Baldwin RM, Lee MG, Taff A, Plopper C, Buckpitt A and Rohde A: Toxicity and metabolism of methylnaphthalenes: Comparison with naphthalene and 1-nitronaphthalene. Toxicology. 260:16–27. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Jong GM, Hsiue TR, Chen CR, Chang HY and Chen CW: Rapidly fatal outcome of bacteremic Klebsiella pneumoniae pneumonia in alcoholics. Chest. 107:214–217. 1995. View Article : Google Scholar : PubMed/NCBI

5 

Paterson DL, Mulazimoglu L, Casellas JM, Ko WC, Goossens H, Von Gottberg A, Mohapatra S, Trenholme GM, Klugman KP, McCormack JG and Yu VL: Epidemiology of ciprofloxacin resistance and its relationship to extended-spectrum beta-lactamase production in Klebsiella pneumoniae isolates causing bacteremia. Clin Infect Dis. 30:473–478. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Bratu S, Landman D, Haag R, Recco R, Eramo A, Alam M and Quale J: Rapid spread of carbapenem-resistant Klebsiella pneumoniae in New York City: A new threat to our antibiotic armamentarium. Arch Intern Med. 165:1430–1435. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Antoniadou A, Kontopidou F, Poulakou G, Koratzanis E, Galani I, Papadomichelakis E, Kopterides P, Souli M, Armaganidis A and Giamarellou H: Colistin-resistant isolates of Klebsiella pneumoniae emerging in intensive care unit patients: First report of a multiclonal cluster. J Antimicrob Chemother. 59:786–790. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Barnes PJ and Karin M: Nuclear factor-kappaB: A pivotal transcription factor in chronic inflammatory diseases. N Engl J Med. 336:1066–1071. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Renard P, Zachary MD, Bougelet C, Mirault ME, Haegeman G, Remacle J and Raes M: Effects of antioxidant enzyme modulations on interleukin-1-induced nuclear factor kappa B activation. Biochem Pharmacol. 53:149–160. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Lentsch AB, Czermak BJ, Bless NM and Ward PA: NF-kappaB activation during IgG immune complex-induced lung injury: Requirements for TNF-alpha and IL-1beta but not complement. Am J Pathol. 152:1327–1336. 1998.PubMed/NCBI

11 

Beg AA, Finco TS, Nantermet PV and AS Jr Baldwin: Tumor necrosis factor and interleukin-1 lead to phosphorylation and loss of I kappa B alpha: A mechanism for NF-kappa B activation. Mol Cell Biol. 13:3301–3310. 1993. View Article : Google Scholar : PubMed/NCBI

12 

Brockman JA, Scherer DC, McKinsey TA, Hall SM, Qi X, Lee WY and Ballard DW: Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation. Mol Cell Biol. 15:2809–2818. 1995. View Article : Google Scholar : PubMed/NCBI

13 

Gloire G, Legrand-Poels S and Piette J: NF-kappaB activation by reactive oxygen species: Fifteen years later. Biochem Pharmacol. 72:1493–1505. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Evans SE, Hahn PY, McCann F, Kottom TJ, Pavlovic ZV and Limper AH: Pneumocystis cell wall beta-glucans stimulate alveolar epithelial cell chemokine generation through nuclear factor-kappaB-dependent mechanisms. Am J Respir Cell Mol Biol. 32:490–497. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Abraham E: The dichotomy of inhibiting nuclear factor kappa-B in pneumonia. Crit Care. 17:1522013. View Article : Google Scholar : PubMed/NCBI

16 

Everhart MB, Han W, Sherrill TP, Arutiunov M, Polosukhin VV, Burke JR, Sadikot RT, Christman JW, Yull FE and Blackwell TS: Duration and intensity of NF-kappaB activity determine the severity of endotoxin-induced acute lung injury. J Immunol. 176:4995–5005. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Bakker-Woudenberg IA, ten Kate MT, Guo L, Working P and Mouton JW: Improved efficacy of ciprofloxacin administered in polyethylene glycol-coated liposomes for treatment of Klebsiella pneumoniae pneumonia in rats. Antimicrob Agents Chemother. 45:1487–1492. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Laichalk LL, Kunkel SL, Strieter RM, Danforth JM, Bailie MB and Standiford TJ: Tumor necrosis factor mediates lung antibacterial host defense in murine Klebsiella pneumonia. Infect Immun. 64:5211–5218. 1996.PubMed/NCBI

19 

Ulich TR, Watson LR, Yin SM, Guo KZ, Wang P, Thang H and del Castillo J: The intratracheal administration of endotoxin and cytokines. I. Characterization of LPS-induced IL-1 and TNF mRNA expression and the LPS-, IL-1-, and TNF-induced inflammatory infiltrate. Am J Pathol. 138:1485–1496. 1991.PubMed/NCBI

20 

Jones MR, Simms BT, Lupa MM, Kogan MS and Mizgerd JP: Lung NF-kappaB activation and neutrophil recruitment require IL-1 and TNF receptor signaling during pneumococcal pneumonia. J Immunol. 175:7530–7535. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Boehm U, Klamp T, Groot M and Howard J: Cellular responses to interferon-gamma. Annu Rev Immunol. 15:749–795. 1997. View Article : Google Scholar : PubMed/NCBI

22 

Maus UA, Waelsch K, Kuziel WA, Delbeck T, Mack M, Blackwell TS, Christman JW, Schlöndorff D, Seeger W and Lohmeyer J: Monocytes are potent facilitators of alveolar neutrophil emigration during lung inflammation: Role of the CCL2-CCR2 axis. J Immunol. 170:3273–3278. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Leung K and Nabel GJ: HTLV-1 transactivator induces interleukin-2 receptor expression through an NF-kappa B-like factor. Nature. 333:776–778. 1988. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu H, Mei B, Wang M and Xu S: Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB. Exp Ther Med 13: 1398-1402, 2017.
APA
Xu, H., Mei, B., Wang, M., & Xu, S. (2017). Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB. Experimental and Therapeutic Medicine, 13, 1398-1402. https://doi.org/10.3892/etm.2017.4130
MLA
Xu, H., Mei, B., Wang, M., Xu, S."Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB". Experimental and Therapeutic Medicine 13.4 (2017): 1398-1402.
Chicago
Xu, H., Mei, B., Wang, M., Xu, S."Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1398-1402. https://doi.org/10.3892/etm.2017.4130
Copy and paste a formatted citation
x
Spandidos Publications style
Xu H, Mei B, Wang M and Xu S: Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB. Exp Ther Med 13: 1398-1402, 2017.
APA
Xu, H., Mei, B., Wang, M., & Xu, S. (2017). Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB. Experimental and Therapeutic Medicine, 13, 1398-1402. https://doi.org/10.3892/etm.2017.4130
MLA
Xu, H., Mei, B., Wang, M., Xu, S."Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB". Experimental and Therapeutic Medicine 13.4 (2017): 1398-1402.
Chicago
Xu, H., Mei, B., Wang, M., Xu, S."Inhibitor κBα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κB". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1398-1402. https://doi.org/10.3892/etm.2017.4130
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