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Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis

  • Authors:
    • Shifeng Ma
    • Xiuhui Chen
    • Lihui Wang
    • Ying Wei
    • Yongqing Ni
    • Yanan Chu
    • Yuanlin Liu
    • Heng Zhu
    • Rongxiu Zheng
    • Yi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Paediatrics, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Postgraduate Studies, Hebei North College, Zhangjiakou, Hebei 075000, P.R. China, Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100085, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1295-1302
    |
    Published online on: February 16, 2017
       https://doi.org/10.3892/etm.2017.4131
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Abstract

The aim of the present study was to determine the repairing effects of intercellular adhesion molecule (ICAM)-1-expressing mesenchymal stem cells (MSCs) in mice with autoimmune thyroiditis. Following induction of an experimental autoimmune thyroiditis (EAT) model, mice were randomly divided into the following groups (n=10 each): i) Normal control; and experimental groups that were subject to EAT induction, including ii) EAT model; and iii) primary MSC; iv) C3H10T1/2/MSC; v) C3H10T1/2‑MIGR1/MSC; and vi) C3H10T1/2‑MIGR1‑ICAM‑1/MSC, which were all administered the relevant cells. MSCs were administered via the caudal vein. A blood sample was harvested from the angular vein of each animal 28 days post-treatment and ELISA was used to determine the serum total triiodothyronine, total thyroxine (T4), thyroid-stimulating hormone (TSH), anti‑thyroid peroxidase (TPOAb), anti‑thyroid microsomal (TMAb) and anti‑thyroglobulin (TGAb) antibodies. Hematoxylin and eosin staining was performed to evaluate injury of the thyroid gland by determining the size of the follicle, inflammatory infiltration, colloidal substance retention and epithelial injury. Reverse transcription‑quantitative polymerase chain reaction was performed to determine the mRNA expression of interleukin (IL)‑4, IL‑10, IL‑17 and interferon (INF)‑γ. Western blot analysis was performed to determine the expression of p38 mitogen-activated protein kinase (p38) and extracellular signal‑regulated kinase (ERK). To observe cellular migration in vivo, mice were divided into the following groups, (n=10 each), which were subject to EAT induction: i) CM-DiI-labeled primary MSC; ii) CM-DiI-labeled C3H10T1/2/MSC; iii) CM‑DiI‑labeled C3H10T1/2‑MIGR1/MSC; and iv) CM-DiI-labeled C3H10T1/2-ICAM‑1/MSC, which were all administered the relevant cells via the caudal vein. C3H10T1/2‑ICAM‑1/MSCs were able to ameliorate the expression of T4, TSH, TPOAb, TMAb and TGAb in vivo, attenuate thyroid follicle injury and decrease the splenic index in mice. They were also able to ameliorate the mRNA expression of IL‑4, IL‑10, IL‑17 and INF‑γ, and the modulation of the P38 and ERK‑signaling pathways in the mouse spleen. Furthermore, ICAM‑1 overexpression was able to modulate the nesting of MSCs in the thyroid gland and lung. These findings suggest that C3H10T1/2-ICAM-1/MSC may affect the differentiation, proliferation and migration of immunocytes through modulating the p38 and ERK signaling pathways, and that ICAM‑1 may modulate the immunoregulatory effects of MSCs by affecting the migration of MSCs in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Ma S, Chen X, Wang L, Wei Y, Ni Y, Chu Y, Liu Y, Zhu H, Zheng R, Zhang Y, Zhang Y, et al: Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis. Exp Ther Med 13: 1295-1302, 2017.
APA
Ma, S., Chen, X., Wang, L., Wei, Y., Ni, Y., Chu, Y. ... Zhang, Y. (2017). Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis. Experimental and Therapeutic Medicine, 13, 1295-1302. https://doi.org/10.3892/etm.2017.4131
MLA
Ma, S., Chen, X., Wang, L., Wei, Y., Ni, Y., Chu, Y., Liu, Y., Zhu, H., Zheng, R., Zhang, Y."Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis". Experimental and Therapeutic Medicine 13.4 (2017): 1295-1302.
Chicago
Ma, S., Chen, X., Wang, L., Wei, Y., Ni, Y., Chu, Y., Liu, Y., Zhu, H., Zheng, R., Zhang, Y."Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1295-1302. https://doi.org/10.3892/etm.2017.4131
Copy and paste a formatted citation
x
Spandidos Publications style
Ma S, Chen X, Wang L, Wei Y, Ni Y, Chu Y, Liu Y, Zhu H, Zheng R, Zhang Y, Zhang Y, et al: Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis. Exp Ther Med 13: 1295-1302, 2017.
APA
Ma, S., Chen, X., Wang, L., Wei, Y., Ni, Y., Chu, Y. ... Zhang, Y. (2017). Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis. Experimental and Therapeutic Medicine, 13, 1295-1302. https://doi.org/10.3892/etm.2017.4131
MLA
Ma, S., Chen, X., Wang, L., Wei, Y., Ni, Y., Chu, Y., Liu, Y., Zhu, H., Zheng, R., Zhang, Y."Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis". Experimental and Therapeutic Medicine 13.4 (2017): 1295-1302.
Chicago
Ma, S., Chen, X., Wang, L., Wei, Y., Ni, Y., Chu, Y., Liu, Y., Zhu, H., Zheng, R., Zhang, Y."Repairing effects of ICAM-1-expressing mesenchymal stem cells in mice with autoimmune thyroiditis". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1295-1302. https://doi.org/10.3892/etm.2017.4131
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