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Article

Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents

  • Authors:
    • Tingting Zhang
    • Min Wang
    • Yixin Xie
    • Xianping Li
    • Zhihui Dong
    • Yanhua Liu
    • Ling Wang
    • Min Yang
    • Huan Song
    • Hong Cao
    • Wei Cao
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 1538-1546
    |
    Published online on: February 21, 2017
       https://doi.org/10.3892/etm.2017.4141
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Abstract

The aim of the present study was to investigate the resistance of Acinetobacter baumannii, which was induced by cefepime (FEP), cefoperazone-sulbactam (SCF), tazobactam (TZP), levofloxacin (LEV), amikacin (AK), imipenem (IPM), and ciprofloxacin (CIP), in vitro. Multi‑step drug resistance selection of 16 A. baumannii strains was performed using seven antibacterial agents (FEP, TZP, CIP, AK, IPM, SCF, and LEV). The minimum inhibitory concentration (MIC) was determined using the agar dilution method. Random amplified polymorphic DNA polymerase chain reaction was performed to analyze the genotypes and the carrying rates of aac(3)‑I, aac(6')‑I, ant(3)‑I, aph(3)‑Via, OXA‑23, OXA‑24, AmpC, TEM‑1, metallo‑β‑lactamase gene (IMP), armA, rmtA, rmtB, parC, gyrA and adeB. Expression of adeB was determined using semi‑quantitative reverse transcription‑polymerase chain reaction (Semi‑qRT‑PCR). Among the 16 strains, 15 strains with drug resistance (93.8%) were obtained following in vitro induction. Notable increases (8‑ to 128-fold) were noted in the MIC and different genotypes were showed in RAPD of the strains before and after performing the drug resistant test. PCR data revealed significant differences (P<0.05) between the carrying rates of resistant genes before and after drug induction, with the exception of rmtA, OXA‑24, TEM‑1, and IMP. Significant increases were demonstrated in the comparative adeB grayscale in strains that underwent drug induction when compared with the sensitive strains (55.69±43.11% vs. 10.08±26.35%; P=0.001). Findings of the present study suggest that the active efflux pump, adeB, has an important role in multidrug resistance of the A. baumannii induced by antibacterial agents in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang T, Wang M, Xie Y, Li X, Dong Z, Liu Y, Wang L, Yang M, Song H, Cao H, Cao H, et al: Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents. Exp Ther Med 13: 1538-1546, 2017.
APA
Zhang, T., Wang, M., Xie, Y., Li, X., Dong, Z., Liu, Y. ... Cao, W. (2017). Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents. Experimental and Therapeutic Medicine, 13, 1538-1546. https://doi.org/10.3892/etm.2017.4141
MLA
Zhang, T., Wang, M., Xie, Y., Li, X., Dong, Z., Liu, Y., Wang, L., Yang, M., Song, H., Cao, H., Cao, W."Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents". Experimental and Therapeutic Medicine 13.4 (2017): 1538-1546.
Chicago
Zhang, T., Wang, M., Xie, Y., Li, X., Dong, Z., Liu, Y., Wang, L., Yang, M., Song, H., Cao, H., Cao, W."Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1538-1546. https://doi.org/10.3892/etm.2017.4141
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang T, Wang M, Xie Y, Li X, Dong Z, Liu Y, Wang L, Yang M, Song H, Cao H, Cao H, et al: Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents. Exp Ther Med 13: 1538-1546, 2017.
APA
Zhang, T., Wang, M., Xie, Y., Li, X., Dong, Z., Liu, Y. ... Cao, W. (2017). Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents. Experimental and Therapeutic Medicine, 13, 1538-1546. https://doi.org/10.3892/etm.2017.4141
MLA
Zhang, T., Wang, M., Xie, Y., Li, X., Dong, Z., Liu, Y., Wang, L., Yang, M., Song, H., Cao, H., Cao, W."Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents". Experimental and Therapeutic Medicine 13.4 (2017): 1538-1546.
Chicago
Zhang, T., Wang, M., Xie, Y., Li, X., Dong, Z., Liu, Y., Wang, L., Yang, M., Song, H., Cao, H., Cao, W."Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1538-1546. https://doi.org/10.3892/etm.2017.4141
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