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Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells

  • Authors:
    • Jing Liu
    • Guang Song
    • Xuewen Lin
    • Xining Pang
    • Tao Meng
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Stem Cells and Regenerative Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1447-1455
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    Published online on: February 21, 2017
       https://doi.org/10.3892/etm.2017.4143
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Abstract

Well-controlled trophoblast invasion at the maternal-fetal interface is crucial for normal placentation and successful pregnancy, otherwise pathological conditions of pregnancy occur, such as preeclampsia. In previous studies, it has been demonstrated that unique long 16 binding protein (ULBP)1, a ligand for the natural‑killer group (NKG)2D receptor on uterine natural killer (uNK) cells, is upregulated in the placenta in patients with preeclampsia. As they are present on the majority of the decidua, uNK have an important role in pregnancy. The aim of the present study was to determine the role of ULBP1 in trophoblast cell invasion, which is closely associated with the occurrence of preeclampsia. In the present study, ULBP1 expression levels in placentas collected after cesarean section from women with preeclampsia and normal pregnant women were determined by immunohistochemistry, reverse transcription‑quantitative polymerase chain reaction and western blotting. The effects of ULBP1 on extravillous trophoblast cell line (HTR‑8/SVneo) invasion mediated via uNK cells and the underlying mechanisms were investigated. mRNA and protein expression levels of ULBP1 were significantly upregulated (P<0.05) in preeclamptic placentas compared with normal controls. ULBP1 inhibited HTR‑8/SVneo cells via the regulation of biological functions of uNK cells, including the downregulation of NKG2D expression on uNK cells and the stimulation of production of cytokines and chemokines that affect extravillous cytotrophoblast invasion by uNK cells. ULBP1 may have an important role in the pathophysiology of preeclampsia through the modification of biological functions of uNK cells, which may affect trophoblast invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Song G, Lin X, Pang X and Meng T: Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells. Exp Ther Med 13: 1447-1455, 2017.
APA
Liu, J., Song, G., Lin, X., Pang, X., & Meng, T. (2017). Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells. Experimental and Therapeutic Medicine, 13, 1447-1455. https://doi.org/10.3892/etm.2017.4143
MLA
Liu, J., Song, G., Lin, X., Pang, X., Meng, T."Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells". Experimental and Therapeutic Medicine 13.4 (2017): 1447-1455.
Chicago
Liu, J., Song, G., Lin, X., Pang, X., Meng, T."Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1447-1455. https://doi.org/10.3892/etm.2017.4143
Copy and paste a formatted citation
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Spandidos Publications style
Liu J, Song G, Lin X, Pang X and Meng T: Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells. Exp Ther Med 13: 1447-1455, 2017.
APA
Liu, J., Song, G., Lin, X., Pang, X., & Meng, T. (2017). Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells. Experimental and Therapeutic Medicine, 13, 1447-1455. https://doi.org/10.3892/etm.2017.4143
MLA
Liu, J., Song, G., Lin, X., Pang, X., Meng, T."Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells". Experimental and Therapeutic Medicine 13.4 (2017): 1447-1455.
Chicago
Liu, J., Song, G., Lin, X., Pang, X., Meng, T."Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1447-1455. https://doi.org/10.3892/etm.2017.4143
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