Open Access

Areca nut extract protects against ovariectomy-induced osteoporosis in mice

  • Authors:
    • Shuyu Li
    • Rong Chen
    • Kaili Luo
    • Yang Guo
    • Man Xiao
    • Guankui Du
  • View Affiliations

  • Published online on: April 19, 2017     https://doi.org/10.3892/etm.2017.4362
  • Pages: 2893-2899
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Estrogen deficiency increases the generation of reactive oxygen species (ROS), which is a crucial pathogenic factor for osteoporosis. Areca nuts are rich in phenolics, which have high antioxidant activity. In the present study, an ovariectomy (OVX)-induced osteoporosis mouse model was used to investigate the protective effects of areca nut extract (ANE) on bone loss and related processes. A total of 24 8‑week‑old female mice were randomly divided into three groups (n=8 per group): I Sham‑operated control; II, bilateral OVX; and III, bilateral OVX + ANE. Group III were treated orally with ANE at a single dose of 300 mg/kg body weight daily for 6 months. ANE supplementation for 6 months improved trabecular bone microarchitecture and significantly increased bone mineral density in the distal femur (P<0.05) compared with Group II. Furthermore, serum levels of the osteoclast differentiation‑inducing factors, receptor activator of nuclear factor‑κB ligand and osteoprotegerin were significantly increased and decreased, respectively (both P<0.05), in OVX mice and these effects were significantly inhibited by ANE treatment (both P<0.05). ANE supplementation also resulted in significantly decreased serum hydrogen peroxide and malondialdehyde levels compared with Group II, while the levels of glutathione and catalase activity were significantly increased (P<0.05 and P<0.01, respectively). The current study indicated that the protective effects of ANE against bone loss were mediated, at least in part, via inhibition of the release of ROS and bone resorption. These results suggested that ANE could have therapeutic value in the treatment of osteoporosis.

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June-2017
Volume 13 Issue 6

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Spandidos Publications style
Li S, Chen R, Luo K, Guo Y, Xiao M and Du G: Areca nut extract protects against ovariectomy-induced osteoporosis in mice. Exp Ther Med 13: 2893-2899, 2017
APA
Li, S., Chen, R., Luo, K., Guo, Y., Xiao, M., & Du, G. (2017). Areca nut extract protects against ovariectomy-induced osteoporosis in mice. Experimental and Therapeutic Medicine, 13, 2893-2899. https://doi.org/10.3892/etm.2017.4362
MLA
Li, S., Chen, R., Luo, K., Guo, Y., Xiao, M., Du, G."Areca nut extract protects against ovariectomy-induced osteoporosis in mice". Experimental and Therapeutic Medicine 13.6 (2017): 2893-2899.
Chicago
Li, S., Chen, R., Luo, K., Guo, Y., Xiao, M., Du, G."Areca nut extract protects against ovariectomy-induced osteoporosis in mice". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2893-2899. https://doi.org/10.3892/etm.2017.4362