Open Access

Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice

  • Authors:
    • Choong‑Gon Kim
    • Ji‑Eun Lee
    • Da‑Geum Jeong
    • Youn‑Ho Lee
    • Sang‑In Park
    • Dae‑Geon Lee
    • Chang‑Hyun Han
    • Su‑Jin Kang
    • Chang‑Hyun Song
    • Seong‑Hun Choi
    • Young‑Joon Lee
    • Sae‑Kwang Ku
  • View Affiliations

  • Published online on: April 27, 2017     https://doi.org/10.3892/etm.2017.4397
  • Pages: 3448-3466
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, it was evaluated whether east saline groundwater concentration solution (ESGWc) exerted a favorable inhibitory effect on 2,4-dinitrochlorobenzene (DNCB)‑induced allergic/atopic‑like dermatitis (AD). AD was induced and boosted by sensitization with DNCB via topical application on the dorsal back skins. Mice with DNCB‑induced AD were bathed in 100‑, 200‑ and 400‑fold diluted ESGWc. After 6 weeks bathing, changes to body weight, clinical skin severity scores, scratching behavior, serum total immunoglobulin (Ig)E levels, submandibular lymph node and spleen weights, splenic cytokine levels, skin cytokine mRNA expressions, antioxidant defense systems and superoxide anion productions were recorded to determine the effects of bathing on the histopathology of dorsal back skin tissues. All DNCB‑induced mice demonstrated that the induction of AD through IgE‑mediated hypersensitivities, oxidative stresses, activation of MMP and apoptosis of keratinocytes resulted in no significant differences in body weight between the different groups at each time point following initial sensitization. However, markers of DNCB‑induced AD were significantly inhibited (P<0.05) in a concentration‑dependent manner following bathing in all concentrations of ESGWc. The results obtained in the present study suggest that bathing in ESGWc may have favorable protective effects against DNCB‑induced AD due to favorable systemic and local immunomodulatory effects, active cytoprotective anti‑apoptotic effects, inhibitory effects of matrix metalloproteinase activity, and anti-inflammatory and antioxidative effects.

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June 2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Kim, C., Lee, J., Jeong, D., Lee, Y., Park, S., Lee, D. ... Ku, S. (2017). Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice. Experimental and Therapeutic Medicine, 13, 3448-3466. https://doi.org/10.3892/etm.2017.4397
MLA
Kim, C., Lee, J., Jeong, D., Lee, Y., Park, S., Lee, D., Han, C., Kang, S., Song, C., Choi, S., Lee, Y., Ku, S."Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice". Experimental and Therapeutic Medicine 13.6 (2017): 3448-3466.
Chicago
Kim, C., Lee, J., Jeong, D., Lee, Y., Park, S., Lee, D., Han, C., Kang, S., Song, C., Choi, S., Lee, Y., Ku, S."Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3448-3466. https://doi.org/10.3892/etm.2017.4397