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Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries

  • Authors:
    • Hai Du
    • Xing Ming
    • Shengkui Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 169-172
    |
    Published online on: May 12, 2017
       https://doi.org/10.3892/etm.2017.4455
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Abstract

Acute cerebral ischemia/reperfusion injury (ACIR) rat model was used to investigate the effect of spermine on oxidative stress and apoptosis. Sixty healthy and clean male Sprague-Dawley rats were randomly divided into 3 groups: The sham-operated group (n=12), the model group (n=12) and the experiment group (n=36). The experiment group was further divided into 3 subgroups: The SPE-10 group (n=12), the SPE-25 group (n=12) and the SPE-50 group (n=12). Rats in the experimental sub-groups SPE-10, SPE-25 and SPE-50 were injected with 10, 25 and 50 mg/kg of spermine, respectively, one week before the establishment of rat models. Rats in the sham‑operated and model groups were injected with 0.9% NaCl solution. We evaluated the effect of spermine on malondialdehyde (MDA) level and superoxide dismutase (SOD) activity using ELISA kits. Bax and Bcl-2 levels were measured using western blot analysis. Our results showed that after spermine injection, MDA levels markedly decreased, while SOD activity increased significantly. The variations in MDA levels and SOD activity were dose (spermine)-dependent. Bax protein levels increased significantly, while Bcl-2 levels decreased significantly after the onset of ACIR injuries. After spermine injection, there was a significant decrease in Bax levels. Bcl-2 levels in these rats markedly increased. The observed decline in Bax levels and the increase in Bcl-2 levels in the experimental groups were dose-dependent. We concluded that spermine protected nerve tissues in rats with ACIR by decreasing the MDA level, increasing SOD activity and modifying the balance between Bax and Bcl-2 proteins.
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Copy and paste a formatted citation
Spandidos Publications style
Du H, Ming X and Zhou S: Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries. Exp Ther Med 14: 169-172, 2017.
APA
Du, H., Ming, X., & Zhou, S. (2017). Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries. Experimental and Therapeutic Medicine, 14, 169-172. https://doi.org/10.3892/etm.2017.4455
MLA
Du, H., Ming, X., Zhou, S."Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries". Experimental and Therapeutic Medicine 14.1 (2017): 169-172.
Chicago
Du, H., Ming, X., Zhou, S."Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries". Experimental and Therapeutic Medicine 14, no. 1 (2017): 169-172. https://doi.org/10.3892/etm.2017.4455
Copy and paste a formatted citation
x
Spandidos Publications style
Du H, Ming X and Zhou S: Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries. Exp Ther Med 14: 169-172, 2017.
APA
Du, H., Ming, X., & Zhou, S. (2017). Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries. Experimental and Therapeutic Medicine, 14, 169-172. https://doi.org/10.3892/etm.2017.4455
MLA
Du, H., Ming, X., Zhou, S."Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries". Experimental and Therapeutic Medicine 14.1 (2017): 169-172.
Chicago
Du, H., Ming, X., Zhou, S."Pre-treatment with spermine for acute cerebral ischemia/reperfusion injuries". Experimental and Therapeutic Medicine 14, no. 1 (2017): 169-172. https://doi.org/10.3892/etm.2017.4455
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