Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells

  • Authors:
    • Zhang‑Yu Bu
    • Li‑Min Wu
    • Xiao‑Hong Yu
    • Jian‑Bo Zhong
    • Ping Yang
    • Jian Chen
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China
    Copyright: © Bu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 303-307
    |
    Published online on: May 16, 2017
       https://doi.org/10.3892/etm.2017.4456
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present investigation explored the in vitro culture, isolation and characterization of hair follicle cell differentiation from umbilical cord blood mesenchymal stem cells (MSCs). Flow cytometry was used to obtain MSCs from the isolation and purification of human umbilical cord blood MSCs. Culture suspension of hair follicle organ was centrifuged and the supernatant used in the culture medium of MSCs, and the entire process of induced differentiation was recorded by photomicroscopy. The expression level of surface marker CK15 of hair follicle cells obtained from induced differentiation was detected with immunofluorescence. RT‑PCR method was used to further detect the difference in expression of CK15 between hair follicle cells and umbilical cord blood MSCs, and statistical analysis was carried out. CD44+CD29+ double‑labeled cells accounted for 50.8% of all the samples of umbilical cord blood MSCs in this study. The diameter of hair follicle cells differentiated from umbilical cord blood stem cells reached 800x10‑3 mm after 3 weeks of cell culture. Based on the detection and colocalization of CK15 expression in induced hair follicle cells, the overlap ratio between CK15 and nuclei reached 83% in hair follicle cells, which was obviously higher than that in umbilical cord blood stem cells. The difference had statistical significance (P<0.05). In conclusion, hair follicle cells can be successfully differentiated from umbilical cord blood stem cells by using the supernatant from hair follicle cells. This method can be used for high‑speed induced differentiation with high purity, which is promising for clinical application.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Codinach M, Blanco M, Ortega I, Lloret M, Reales L, Coca MI, Torrents S, Doral M, Oliver-Vila I, Requena-Montero M, et al: Design and validation of a consistent and reproducible manufacture process for the production of clinical-grade bone marrow-derived multipotent mesenchymal stromal cells. Cytotherapy. 18:1197–1208. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Kumar K, Agarwal P, Das K, Mili B, Madhusoodan AP, Kumar A and Bag S: Isolation and characterization of mesenchymal stem cells from caprine umbilical cord tissue matrix. Tissue Cell. 48:653–658. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Del Valle-Echevarria AR, Sanseverino W, Garcia-Mas J and Havey MJ: Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber. Theor Appl Genet. 129:1951–1959. 2016. View Article : Google Scholar : PubMed/NCBI

4 

de Moor JS, Dowling EC, Ekwueme DU, Guy GP Jr, Rodriguez J, Virgo KS, Han X, Kent EE, Li C, Litzelman K, et al: Employment implications of informal cancer caregiving. J Cancer Surviv. 11:48–57. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Sigurjónsson OE, Guðmundsson KO and Guðmundsson S: Mesenchymal stem cells. A review. Laeknabladid. 87:627–632. 2001.(In Icelandic). PubMed/NCBI

6 

Maranda EL, Rodriguez-Menocal L and Badiavas EV: Role of mesenchymal stem cells in dermal repair in burns and diabetic wounds. Curr Stem Cell Res Ther. 12:61–70. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Astori G, Amati E, Bambi F, Bernardi M, Chieregato K, Schäfer R, Sella S and Rodeghiero F: Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: Present and future. Stem Cell Res Ther. 7:93–100. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Dyrna F, Herbst E, Hoberman A, Imhoff AB and Schmitt A: Stem cell procedures in arthroscopic surgery. Eur J Med Res. 21:29–36. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Jiang LH, Hao Y, Mousawi F, Peng H and Yang X: Expression of P2 purinergic receptors in mesenchymal stem cells and their roles in extracellular nucleotide regulation of cell functions. J Cell Physiol. 232:287–297. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Morris AD, Chen J, Lau E and Poh J: Domperidone-associated QT interval prolongation in non-oncologic pediatric patients: A review of the literature. Can J Hosp Pharm. 69:224–230. 2016.PubMed/NCBI

11 

Kavosi Z, Khorrami M Sarikhani, Keshavarz K, Jafari A, Meshkini A Hashemi, Safaei HR and Nikfar S: Is taurolidine-citrate an effective and cost-effective hemodialysis catheter lock solution? A systematic review and cost-effectiveness analysis. Med J Islam Repub Iran. 30:347–358. 2016.PubMed/NCBI

12 

Shi X, Lv S, He X, Liu X, Sun M, Li M, Chi G and Li Y: Differentiation of hepatocytes from induced pluripotent stem cells derived from human hair follicle mesenchymal stem cells. Cell Tissue Res. 366:89–99. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Maruyama CL, Leigh NJ, Nelson JW, McCall AD, Mellas RE, Lei P, Andreadis ST and Baker OJ: Stem cell-soluble signals enhance multilumen formation in SMG cell clusters. J Dent Res. 94:1610–1617. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Son S, Liang MS, Lei P, Xue X, Furlani EP and Andreadis ST: Magnetofection mediated transient NANOG overexpression enhances proliferation and myogenic differentiation of human hair follicle derived mesenchymal stem cells. Bioconjug Chem. 26:1314–1327. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Maleki M, Ghanbarvand F, Behvarz M Reza, Ejtemaei M and Ghadirkhomi E: Comparison of mesenchymal stem cell markers in multiple human adult stem cells. Int J Stem Cells. 7:118–126. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Dong L, Hao H, Xia L, Liu J, Ti D, Tong C, Hou Q, Han Q, Zhao Y, Liu H, et al: Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth. Sci Rep. 4:5432–5440. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wu M, Guo X, Yang L, Wang Y, Tang Y, Yang Y and Liu H: Mesenchymal stem cells with modification of junctional adhesion molecule a induce hair formation. Stem Cells Transl Med. 3:481–488. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Wang Y, Liu J, Tan X, Li G, Gao Y, Liu X, Zhang L and Li Y: Induced pluripotent stem cells from human hair follicle mesenchymal stem cells. Stem Cell Rev. 9:451–460. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Gola M, Czajkowski R, Bajek A, Dura A and Drewa T: Melanocyte stem cells: Biology and current aspects. Med Sci Monit. 18:RA155–RA159. 2012. View Article : Google Scholar : PubMed/NCBI

20 

DiDomenico L, Landsman AR, Emch KJ and Landsman A: A prospective comparison of diabetic foot ulcers treated with either a cryopreserved skin allograft or a bioengineered skin substitute. Wounds. 23:184–189. 2011.PubMed/NCBI

21 

Liang MS and Andreadis ST: Engineering fibrin-binding TGF-β1 for sustained signaling and contractile function of MSC based vascular constructs. Biomaterials. 32:8684–8693. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Bu ZY, Wu LM, Yu XH, Zhong JB, Yang P and Chen J: Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells. Exp Ther Med 14: 303-307, 2017.
APA
Bu, Z., Wu, L., Yu, X., Zhong, J., Yang, P., & Chen, J. (2017). Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells. Experimental and Therapeutic Medicine, 14, 303-307. https://doi.org/10.3892/etm.2017.4456
MLA
Bu, Z., Wu, L., Yu, X., Zhong, J., Yang, P., Chen, J."Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells". Experimental and Therapeutic Medicine 14.1 (2017): 303-307.
Chicago
Bu, Z., Wu, L., Yu, X., Zhong, J., Yang, P., Chen, J."Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells". Experimental and Therapeutic Medicine 14, no. 1 (2017): 303-307. https://doi.org/10.3892/etm.2017.4456
Copy and paste a formatted citation
x
Spandidos Publications style
Bu ZY, Wu LM, Yu XH, Zhong JB, Yang P and Chen J: Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells. Exp Ther Med 14: 303-307, 2017.
APA
Bu, Z., Wu, L., Yu, X., Zhong, J., Yang, P., & Chen, J. (2017). Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells. Experimental and Therapeutic Medicine, 14, 303-307. https://doi.org/10.3892/etm.2017.4456
MLA
Bu, Z., Wu, L., Yu, X., Zhong, J., Yang, P., Chen, J."Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells". Experimental and Therapeutic Medicine 14.1 (2017): 303-307.
Chicago
Bu, Z., Wu, L., Yu, X., Zhong, J., Yang, P., Chen, J."Isolation and characterization of in vitro culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells". Experimental and Therapeutic Medicine 14, no. 1 (2017): 303-307. https://doi.org/10.3892/etm.2017.4456
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team