Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin

  • Authors:
    • Bei Zhang
    • Yongquan Shi
    • Junjie Zou
    • Xiangfang Chen
    • Wei Tang
    • Fei Ye
    • Zhimin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Shanghai Changzheng Hospital, Shanghai 200003, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 135-140
    |
    Published online on: May 17, 2017
       https://doi.org/10.3892/etm.2017.4458
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

ATP-sensitive potassium (KATP) channels are well characterized in cardiac, pancreatic and many other muscle cells. The purpose of this study was to determine if KATP channels play a role in diabetic nephropathy (DN). In the present study, functional expression of the KATP channel was examined in rat mesangial cells with or without high glucose (HG) stimulation. The mesangial cell proliferation and the release of matrix metalloproteinase (MMP)-2 and fibronectin in response to high glucose with a selective opener of KATP (diazoxide, DZX), or with a selective inhibitor of KATP (5-hydroxydecanoate, 5-HD) were also measured. The cell proliferation was observed using Cell Counting Kit-8 assay, and the mRNA expressions of KATP subunit, including Kir6.1, Kir6.2, sulfonylurea receptor 1 (SUR1), SUR2A and SUR2B, were assessed using quantitative real-time PCR. MMP-2 and fibronectin release was measured by ELISA. The present study clarified expression of SUR subunit of KATP in plasma. HG treatment could cause increased cell proliferation and release of MMP-2 and fibronectin in a dose-dependent manner. HG also significantly decreased the expression of Kir6.1, SUR2A and SUR2B. Pretreatment of DZX markedly decreased the expression of SUR1, SUR2A and SUR2B, but had no effect on Kir6.1 expression compared with HG alone, while these changes were inhibited by 5-HD pretreatment. Moreover, DZX also inhibited cell proliferation and release of MMP-2 and fibronectin in HG-induced rat mesangial cells, and that was corrected by 5-HD. These data suggest that HG stimulates mesangial cell proliferation and cellular matrix release via inhibiting KATP channel activity, leading us to propose that KATP channel dysfunction may be involved in the development of DN.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Aghadavod E, Khodadadi S, Baradaran A, Nasri P, Bahmani M and Rafieian-Kopaei M: Role of oxidative stress and inflammatory factors in diabetic kidney disease. Iran J Kidney Dis. 10:337–343. 2016.PubMed/NCBI

2 

John S: Complication in diabetic nephropathy. Diabetes Metab Syndr. 10:247–249. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Kolset SO, Reinholt FP and Jenssen T: Diabetic nephropathy and extracellular matrix. J Histochem Cytochem. 60:976–986. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Ruster C and Wolf G: The role of chemokines and chemokine receptors in diabetic nephropathy. Front Biosci. 13:944–955. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Kikkawa R, Koya D and Haneda M: Progression of diabetic nephropathy. Am J Kidney Dis. 41 Suppl 1:S19–S21. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Zhang L, Pang S, Deng B, Qian L, Chen J, Zou J, Zheng J, Yang L, Zhang C, Chen X, et al: High glucose induces renal mesangial cell proliferation and fibronectin expression through JNK/NF-κB/NADPH oxidase/ROS pathway, which is inhibited by resveratrol. Int J Biochem Cell Biol. 44:629–638. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Li X, Liu W, Wang Q, Liu P, Deng Y, Lan T, Zhang X, Qiu B, Ning H and Huang H: Emodin suppresses cell proliferation and fibronectin expression via p38MAPK pathway in rat mesangial cells cultured under high glucose. Mol Cell Endocrinol. 307:157–162. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Tan RJ and Liu Y: Matrix metalloproteinases in kidney homeostasis and diseases. Am J Physiol Renal Physiol. 302:F1351–F1361. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Gharagozlian S, Svennevig K, Bangstad HJ, Winberg JO and Kolset SO: Matrix metalloproteinases in subjects with type 1 diabetes. BMC Clin Pathol. 9:72009. View Article : Google Scholar : PubMed/NCBI

10 

Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B and Tannock LR: Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy. Am J Pathol. 179:1179–1187. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Burke MA, Mutharasan RK and Ardehali H: The sulfonylurea receptor, an atypical ATP-binding cassette protein, and its regulation of the KATP channel. Circ Res. 102:164–176. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Wheeler A, Wang C, Yang K, Fang K, Davis K, Styer AM, Mirshahi U, Moreau C, Revilloud J, Vivaudou M, et al: Coassembly of different sulfonylurea receptor subtypes extends the phenotypic diversity of ATP-sensitive potassium (KATP) channels. Mol Pharmacol. 74:1333–1344. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Teramoto N, Zhu HL, Shibata A, Aishima M, Walsh EJ, Nagao M and Cole WC: ATP-sensitive K+ channels in pig urethral smooth muscle cells are heteromultimers of Kir6.1 and Kir6.2. Am J Physiol Renal Physiol. 296:F107–F117. 2009. View Article : Google Scholar : PubMed/NCBI

14 

McTaggart JS, Clark RH and Ashcroft FM: The role of the KATP channel in glucose homeostasis in health and disease: more than meets the islet. J Physiol. 588:3201–3209. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Yang SN, Wenna ND, Yu J, Yang G, Qiu H, Yu L, Juntti-Berggren L, Köhler M and Berggren PO: Glucose recruits K(ATP) channels via non-insulin-containing dense-core granules. Cell Metab. 6:217–228. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Hu K, Huang CS, Jan YN and Jan LY: ATP-sensitive potassium channel traffic regulation by adenosine and protein kinase C. Neuron. 38:417–432. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Chen PC, Kryukova YN and Shyng SL: Leptin regulates KATP channel trafficking in pancreatic β-cells by a signaling mechanism involving AMP-activated protein kinase (AMPK) and cAMP-dependent protein kinase (PKA). J Biol Chem. 288:34098–34109. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Masia R, Enkvetchakul D and Nichols CG: Differential nucleotide regulation of KATP channels by SUR1 and SUR2A. J Mol Cell Cardiol. 39:491–501. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Hong SH, Kyeong KS, Kim CH, Kim YC, Choi W, Yoo RY, Kim HS, Park YJ, Ji IW, Jeong EH, et al: Regulation of myometrial contraction by ATP-sensitive potassium (KATP) channel via activation of SUR2B and Kir 6.2 in mouse. J Vet Med Sci. 78:1153–1159. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Zhang H, Flagg TP and Nichols CG: Cardiac sarcolemmal K(ATP) channels: latest twists in a questing tale! J Mol Cell Cardiol. 48:71–75. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Pu JL, Ye B, Kroboth SL, McNally EM, Makielski JC and Shi NQ: Cardiac sulfonylurea receptor short form-based channels confer a glibenclamide-insensitive KATP activity. J Mol Cell Cardiol. 44:188–200. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Elrod JW, Harrell M, Flagg TP, Gundewar S, Magnuson MA, Nichols CG, Coetzee WA and Lefer DJ: Role of sulfonylurea receptor type 1 subunits of ATP-sensitive potassium channels in myocardial ischemia/reperfusion injury. Circulation. 117:1405–1413. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Lefer DJ, Nichols CG and Coetzee WA: Sulfonylurea receptor 1 subunits of ATP-sensitive potassium channels and myocardial ischemia/reperfusion injury. Trends Cardiovasc Med. 19:61–67. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Yuan P, Xue H, Zhou L, Qu L, Li C, Wang Z, Ni J, Yu C, Yao T, Huang Y, et al: Rescue of mesangial cells from high glucose-induced over-proliferation and extracellular matrix secretion by hydrogen sulfide. Nephrol Dial Transplant. 26:2119–2126. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Rysz J, Banach M, Stolarek RA, Pasnik J, Cialkowska-Rysz A, Koktysz R, Piechota M and Baj Z: Serum matrix metalloproteinases MMP-2 and MMP-9 and metalloproteinase tissue inhibitors TIMP-1 and TIMP-2 in diabetic nephropathy. J Nephrol. 20:444–452. 2007.PubMed/NCBI

27 

Wang J, Huang H, Liu P, Tang F, Qin J, Huang W, Chen F, Guo F, Liu W and Yang B: Inhibition of phosphorylation of p38 MAPK involved in the protection of nephropathy by emodin in diabetic rats. Eur J Pharmacol. 553:297–303. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Hopps E and Caimi G: Matrix metalloproteinases in metabolic syndrome. Eur J Intern Med. 23:99–104. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Tarasov AI, Girard CA and Ashcroft FM: ATP sensitivity of the ATP-sensitive K+ channel in intact and permeabilized pancreatic beta-cells. Diabetes. 55:2446–2454. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Liang W, Chen J, Mo L, Ke X, Zhang W, Zheng D, Pan W, Wu S, Feng J, Song M, et al: ATP-sensitive K+ channels contribute to the protective effects of exogenous hydrogen sulfide against high glucose-induced injury in H9c2 cardiac cells. Int J Mol Med. 37:763–772. 2016.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Shi Y, Zou J, Chen X, Tang W, Ye F and Liu Z: KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin. Exp Ther Med 14: 135-140, 2017.
APA
Zhang, B., Shi, Y., Zou, J., Chen, X., Tang, W., Ye, F., & Liu, Z. (2017). KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin. Experimental and Therapeutic Medicine, 14, 135-140. https://doi.org/10.3892/etm.2017.4458
MLA
Zhang, B., Shi, Y., Zou, J., Chen, X., Tang, W., Ye, F., Liu, Z."KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin". Experimental and Therapeutic Medicine 14.1 (2017): 135-140.
Chicago
Zhang, B., Shi, Y., Zou, J., Chen, X., Tang, W., Ye, F., Liu, Z."KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin". Experimental and Therapeutic Medicine 14, no. 1 (2017): 135-140. https://doi.org/10.3892/etm.2017.4458
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Shi Y, Zou J, Chen X, Tang W, Ye F and Liu Z: KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin. Exp Ther Med 14: 135-140, 2017.
APA
Zhang, B., Shi, Y., Zou, J., Chen, X., Tang, W., Ye, F., & Liu, Z. (2017). KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin. Experimental and Therapeutic Medicine, 14, 135-140. https://doi.org/10.3892/etm.2017.4458
MLA
Zhang, B., Shi, Y., Zou, J., Chen, X., Tang, W., Ye, F., Liu, Z."KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin". Experimental and Therapeutic Medicine 14.1 (2017): 135-140.
Chicago
Zhang, B., Shi, Y., Zou, J., Chen, X., Tang, W., Ye, F., Liu, Z."KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin". Experimental and Therapeutic Medicine 14, no. 1 (2017): 135-140. https://doi.org/10.3892/etm.2017.4458
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team