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Article Open Access

Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis

  • Authors:
    • Wei Hao
    • Chuanqiang Jiang
    • Ming Jiang
    • Tian Wang
    • Xin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics and Traumatology, Yantai Yuhuangding Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong 264000, P.R. China, Department of Orthopedics and Traumatology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China, Department of Stomatology, 107 Hospital of Jinan Military Area, Yantai, Shandong 264002, P.R. China, Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China
    Copyright: © Hao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 43-50
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    Published online on: May 17, 2017
       https://doi.org/10.3892/etm.2017.4465
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Abstract

Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose‑derived stem cells (opASCs) were harvested from subcutaneous adipose tissue. Mature adipocytes were dedifferentiated to produce opDFATs by the ceiling culture method. OpDFATs were osteogenically induced in vitro with opASCs as a control. Cell growth, alkaline phosphatase (ALPase) activity and cell mineralization were determined, and expression levels of osteogenesis‑specific genes (collagen I, osteocalcin and bone sialoprotein) were analyzed using quantitative reverse transcription polymerase chain reaction. After 14 days, the opDFATs were combined with a poly(lactide‑co‑glycolide)‑β‑tricalcium phosphate porous scaffold after being suspended in collagen I gel and implanted into nude mice for 4 weeks prior to histological analysis. Unilocular lipid droplets in mature adipocytes gradually split into smaller droplets and disappeared from the cytoplasm. Mature adipocytes dedifferentiated to opDFATs and cell morphology changed from spherical to elongated. High levels of ALPase and cell mineralization were observed in opDFATs by staining. No significant differences were found between the growth curves, ALPase activity, cell mineralization and expression levels of osteogenesis‑specific genes between opDFATS and opASCs. After implantation for 4 weeks, new bone tissue was observed histologically in the opDFATs‑based biocomposite. OpDFATs are implicated as a novel type of seed cell for bone tissue engineering based on their osteogenic potency and higher abundance in adipose tissue compared with opASCs.
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1 

Jiang M, Wang X, Liu H, Zhou L, Jiang T, Zhou H and Hao W: Bone formation in adipose-derived stem cells isolated from elderly patients with osteoporosis: A preliminary study. Cell Biol Int. 38:97–105. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Hao W, Hu YY, Wei YY, Pang L, Lv R, Bai JP, Xiong Z and Jiang M: Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold. Cells Tissues Organs. 187:89–102. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Lee JH, Lee JH, Park JW and Shin YH: The insertional torque of a pedicle screw has a positive correlation with bone mineral density in posterior lumbar pedicle screw fixation. J Bone Joint Surg Br. 94:93–97. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Wu ZX, Gong FT, Liu L, Ma ZS, Zhang Y, Zhao X, Yang M, Lei W and Sang HX: A comparative study on screw loosening in osteoporotic lumbar spine fusion between expandable and conventional pedicle screws. Arch Orthop Trauma Surg. 132:471–476. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Hao W, Pang L, Jiang M, Lv R, Xiong Z and Hu YY: Skeletal repair in rabbits using a novel biomimetic composite based on adipose-derived stem cells encapsulated in collagen I gel with PLGA-beta-TCP scaffold. J Orthop Res. 28:252–257. 2010.PubMed/NCBI

6 

Hao W, Dong J, Jiang M, Wu J, Cui F and Zhou D: Enhanced bone formation in large segmental radial defects by combining adipose-derived stem cells expressing bone morphogenetic protein 2 with nHA/RHLC/PLA scaffold. Int Orthop. 34:1341–1349. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Rosen CJ and Bouxsein ML: Mechanisms of disease: Is osteoporosis the obesity of bone? Nat Clin Pract Rheumatol. 2:35–43. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Hagey AR and Warren MP: Role of exercise and nutrition in menopause. Clin Obstet Gynecol. 51:627–641. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Carbonare L Dalle, Valenti MT, Zanatta M, Donatelli L and Lo Cascio V: Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis. Arthritis Rheum. 60:3356–3365. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Chen JR, Lazarenko OP, Wu X, Tong Y, Blackburn ML, Shankar K, Badger TM and Ronis MJ: Obesity reduces bone density associated with activation of PPARγ and suppression of Wnt/β-catenin in rapidly growing male rats. PLoS One. 5:e137042010. View Article : Google Scholar : PubMed/NCBI

11 

Chen HT, Lee MJ, Chen CH, Chuang SC, Chang LF, Ho ML, Hung SH, Fu YC, Wang YH, Wang HI, et al: Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures. J Cell Mol Med. 16:582–593. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Wu W, Niklason L and Steinbacher DM: The effect of age on human adipose-derived stem cells. Plast Reconstr Surg. 131:27–37. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Park SR, Oreffo RO and Triffitt JT: Interconversion potential of cloned human marrow adipocytes in vitro. Bone. 24:549–554. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Justesen J, Pedersen SB, Stenderup K and Kassem M: Subcutaneous adipocytes can differentiate into bone-forming cells in vitro and in vivo. Tissue Eng. 10:381–391. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Poloni A, Maurizi G, Leoni P, Serrani F, Mancini S, Frontini A, Zingaretti MC, Siquini W, Sarzani R and Cinti S: Human dedifferentiated adipocytes show similar properties to bone marrow-derived mesenchymal stem cells. Stem Cells. 30:965–974. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Kikuta S, Tanaka N, Kazama T, Kazama M, Kano K, Ryu J, Tokuhashi Y and Matsumoto T: Osteogenic effects of dedifferentiated fat cell transplantation in rabbit models of bone defect and ovariectomy-induced osteoporosis. Tissue Eng Part A. 19:1792–1802. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Hodson AW and Skillen AW: Comparison of diazo-coupling, formazan, and silver staining techniques for visualizing alkaline phosphatase isoenzymes after electrophoresis in homogeneous-pore and gradient-pore polyacrylamide gels. Anal Biochem. 169:253–261. 1988. View Article : Google Scholar : PubMed/NCBI

18 

Mallory FB: Pathological techniques: A practical manual for workers in pathological histology including directions for the performance of autopsies and for microphotography. WB Saunders, Philadelphia, PA; pp. 143–144. 1983

19 

Stolzing A, Jones E, McGonagle D and Scutt A: Age-related changes in human bone marrow-derived mesenchymal stem cells: Consequences for cell therapies. Mech Ageing Dev. 129:163–173. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Zhou S, Greenberger JS, Epperly MW, Goff JP, Adler C, Leboff MS and Glowacki J: Age-related intrinsic changes in human bone-marrow-derived mesenchymal stem cells and their differentiation to osteoblasts. Aging cell. 7:335–343. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Riis BJ, Rødbro P and Christiansen C: The role of serum concentrations of sex steroids and bone turnover in the development and occurrence of postmenopausal osteoporosis. Calcif Tissue Int. 38:318–322. 1986. View Article : Google Scholar : PubMed/NCBI

22 

Lau EM, Chan YH, Chan M, Woo J, Griffith J, Chan HH and Leung PC: Vertebral deformity in chinese men: Prevalence, risk factors, bone mineral density, and body composition measurements. Calcif Tissue Int. 66:47–52. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Hsu YH, Venners SA, Terwedow HA, Feng Y, Niu T, Li Z, Laird N, Brain JD, Cummings SR, Bouxsein ML, et al: Relation of body composition, fat mass, and serum lipids to osteoporotic fractures and bone mineral density in Chinese men and women. Am J Clin Nutr. 83:146–154. 2006.PubMed/NCBI

24 

Zhao LJ, Liu YJ, Liu PY, Hamilton J, Recker RR and Deng HW: Relationship of obesity with osteoporosis. J Clin Endocrinol Metab. 92:1640–1646. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Zhao LJ, Jiang H, Papasian CJ, Maulik D, Drees B, Hamilton J and Deng HW: Correlation of obesity and osteoporosis: Effect of fat mass on the determination of osteoporosis. J Bone Miner Res. 23:17–29. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Matsumoto T, Kano K, Kondo D, Fukuda N, Iribe Y, Tanaka N, Matsubara Y, Sakuma T, Satomi A, Otaki M, et al: Mature adipocyte-derived dedifferentiated fat cells exhibit multilineage potential. J Cell Physiol. 215:210–222. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Schäffler A and Büchler C: Concise review: Adipose tissue-derived stromal cells-basic and clinical implications for novel cell-based therapies. Stem cells. 25:818–827. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP and Hedrick MH: Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Yoshimura K, Shigeura T, Matsumoto D, Sato T, Takaki Y, Aiba-Kojima E, Sato K, Inoue K, Nagase T, Koshima I and Gonda K: Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol. 208:64–76. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Kazama T, Fujie M, Endo T and Kano K: Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro. Biochem Biophys Res Commun. 377:780–785. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Oki Y, Watanabe S, Endo T and Kano K: Mature adipocyte-derived dedifferentiated fat cells can trans-differentiate into osteoblasts in vitro and in vivo only by all-trans retinoic acid. Cell Struct Funct. 33:211–222. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Jumabay M, Matsumoto T, Yokoyama S, Kano K, Kusumi Y, Masuko T, Mitsumata M, Saito S, Hirayama A, Mugishima H and Fukuda N: Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats. J Mol Cell Cardiol. 47:565–575. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Sakuma T, Matsumoto T, Kano K, Fukuda N, Obinata D, Yamaguchi K, Yoshida T, Takahashi S and Mugishima H: Mature, adipocyte derived, dedifferentiated fat cells can differentiate into smooth muscle-like cells and contribute to bladder tissue regeneration. J Urol. 182:355–365. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Nakamura T, Shinohara Y, Momozaki S, Yoshimoto T and Noguchi K: Co-stimulation with bone morphogenetic protein-9 and FK506 induces remarkable osteoblastic differentiation in rat dedifferentiated fat cells. Biochem Biophys Res Commun. 440:289–294. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Liu HY, Wu AT, Tsai CY, Chou KR, Zeng R, Wang MF, Chang WC, Hwang SM, Su CH and Deng WP: The balance between adipogenesis and osteogenesis in bone regeneration by platelet-rich plasma for age-related osteoporosis. Biomaterials. 32:6773–6780. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Xiong Z, Yan Y, Wang S, Zhang R and Zhang C: Fabrication of porous scaffolds for bone tissue engineering via low-temperature deposition. Scripta Materialia. 46:771–776. 2002. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Hao W, Jiang C, Jiang M, Wang T and Wang X: Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis. Exp Ther Med 14: 43-50, 2017.
APA
Hao, W., Jiang, C., Jiang, M., Wang, T., & Wang, X. (2017). Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis. Experimental and Therapeutic Medicine, 14, 43-50. https://doi.org/10.3892/etm.2017.4465
MLA
Hao, W., Jiang, C., Jiang, M., Wang, T., Wang, X."Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis". Experimental and Therapeutic Medicine 14.1 (2017): 43-50.
Chicago
Hao, W., Jiang, C., Jiang, M., Wang, T., Wang, X."Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis". Experimental and Therapeutic Medicine 14, no. 1 (2017): 43-50. https://doi.org/10.3892/etm.2017.4465
Copy and paste a formatted citation
x
Spandidos Publications style
Hao W, Jiang C, Jiang M, Wang T and Wang X: Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis. Exp Ther Med 14: 43-50, 2017.
APA
Hao, W., Jiang, C., Jiang, M., Wang, T., & Wang, X. (2017). Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis. Experimental and Therapeutic Medicine, 14, 43-50. https://doi.org/10.3892/etm.2017.4465
MLA
Hao, W., Jiang, C., Jiang, M., Wang, T., Wang, X."Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis". Experimental and Therapeutic Medicine 14.1 (2017): 43-50.
Chicago
Hao, W., Jiang, C., Jiang, M., Wang, T., Wang, X."Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis". Experimental and Therapeutic Medicine 14, no. 1 (2017): 43-50. https://doi.org/10.3892/etm.2017.4465
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