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Article

(-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts

  • Authors:
    • Kazuhiko Fujita
    • Takanobu Otsuka
    • Naohiro Yamamoto
    • Shingo Kainuma
    • Reou Ohguchi
    • Tetsu Kawabata
    • Go Sakai
    • Gen Kuroyanagi
    • Rie Matsushima‑Nishiwaki
    • Osamu Kozawa
    • Haruhiko Tokuda
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan
  • Pages: 417-423
    |
    Published online on: May 22, 2017
       https://doi.org/10.3892/etm.2017.4491
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Abstract

Chlorogenic acid (CGA) is a primary phenolic component of coffee and (‑)‑epigallocatechin gallate (EGCG) is a primary flavonoid component of green tea, both of which have been documented to possess beneficial health properties. A previous study by the present authors demonstrated that p38 mitogen‑activated protein kinase (MAPK) may be associated with osteoprotegerin synthesis stimulated by bone morphogenetic protein‑4 (BMP‑4) in osteoblast‑like MC3T3‑E1 cells. In the present study, the effects of CGA and EGCG on BMP‑4‑stimulated osteoprotegerin synthesis in MC3T3‑E1 cells were investigated. It was observed that CGA had no effect on osteoprotegerin release stimulated by BMP‑4, whereas EGCG significantly enhanced BMP‑4‑stimulated osteoprotegerin release (P=0.003). Levels of osteoprotegerin mRNA expression induced by BMP‑4 were also significantly increased by EGCG (P=0.03). By contrast, EGCG had no significant effect on phosphorylation of Smad1 or p38 MAPK induced by BMP‑4. In addition, EGCG had little effect on BMP‑induced phosphorylation of p70 S6 kinase; however rapamycin, as an inhibitor of p70 S6 kinase, significantly suppressed osteoprotegerin release (P=0.007). These data suggest that EGCG but not CGA may upregulate the synthesis of osteoprotegerin induced by BMP-4 in osteoblasts.
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1 

Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Lüthy R, Nguyen HQ, Wooden S, Bennett L, Boone T, et al: Osteoprotegerin: A novel secreted protein involved in the regulation of bone density. Cell. 89:309–319. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Karsenty G and Wagner EF: Reaching a genetic and molecular understanding of skeletal development. Dev Cell. 2:389–406. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Zuo C, Huang Y, Bajis R, Sahih M, Li YP, Dai K and Zhang X: Osteoblastgenesis regulation signals in bone remodeling. Osteoporos Int. 23:1653–1663. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Hadjidakis DJ and Androulakis II: Bone remodeling. Ann N Y Acad Sci. 1092:385–396. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Rachner TD, Khosla S and Hofbauer LC: Osteoporosis: Now and the future. Lancet. 377:1276–1287. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Seitz S, Priemel M, Zustin J, Beil FT, Semler J, Minne H, Schinke T and Amling M: Paget's disease of bone: Histologic analysis of 754 patients. J Bone Miner Res. 24:62–69. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Tat S Kwan, Padrines M, Théoleyre S, Heymann D and Fortun Y: IL-6, RANKL, TNF-alpha/IL-1: Interrelations in bone resorption pathophysiology. Cytokine Growth Factor Rev. 15:49–60. 2004. View Article : Google Scholar : PubMed/NCBI

8 

George SE, Ramalakshmi K and Rao LJ Mohan: A perception on health benefits of coffee. Crit Rev Food Sci Nutr. 48:464–486. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Thielecke F and Boschmann M: The potential role of green tea catechins in the prevention of the metabolic syndrome-a review. Phytochemistry. 70:11–24. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Shimizu M, Adachi S, Masuda M, Kozawa O and Moriwaki H: Cancer chemoprevention with green tea catechins by targeting receptor tyrosine kinases. Mol Nutr Food Res. 55:832–843. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Folwarczna J, Pytlik M, Zych M, Cegieła U, Nowinska B, Kaczmarczyk-Sedlak I, Sliwinski L, Trzeciak H and Trzeciak HI: Effects of caffeic and chlorogenic acids on the rat skeletal system. Eur Rev Med Pharmacol Sci. 19:682–693. 2015.PubMed/NCBI

12 

Kwak SC, Lee C, Kim JY, Oh HM, So HS, Lee MS, Rho MC and Oh J: Chlorogenic acid inhibits osteoclast differentiation and bone resorption by down-regulation of receptor activator of nuclear factor kappa-B ligand-induced nuclear factor of activated T cells c1 expression. Biol Pharm Bull. 36:1779–1786. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Shen CL, Yeh JK, Cao JJ and Wang JS: Green tea and bone metabolism. Nutr Res. 29:437–456. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Singh R, Akhtar N and Haqqi TM: Green tea polyphenol epigallocatechin-3-gallate: Inflammation and arthritis. (corrected). Life Sci. 86:907–918. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Miyazono K, Maeda S and Imamura T: BMP receptor signaling: Transcriptional targets, regulation of signals, and signaling cross-talk. Cytokine Growth Factor Rev. 16:251–263. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Krause C, De Gorter DJJ, Karperien M and ten Dijke P: Signal transduction cascades controlling osteoblast differentiationPrimer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. Rosen CJ: 7th. John Wiley & Sons, Inc.; Washington, DC: 2008, View Article : Google Scholar

17 

Moustakas A and Heldin CH: Non-Smad TGF-beta signals. J Cell Sci. 118:3573–3584. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Cai J, Pardali E, Sánchez-Duffhues G and ten Dijke P: BMP signaling in vascular disease. FEBS Lett. 586:1993–2002. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Kuroyanagi G, Tokuda H, Yamamoto N, Matsushima-Nishiwaki R, Mizutani J, Kozawa O and Otsuka T: Resveratrol amplifies BMP-4-stimulated osteoprotegerin synthesis via p38 MAPK in osteoblasts. Mol Med Rep. 12:3849–3854. 2015.PubMed/NCBI

20 

Sudo H, Kodama HA, Amagai Y, Yamamoto S and Kasai S: In vivo differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI

21 

Kozawa O, Tokuda H, Miwa M, Kotoyori J and Oiso Y: Cross-talk regulation between cyclic AMP production and phosphoinositide hydrolysis induced by prostaglandin E2 in osteoblast-like cells. Exp Cell Res. 198:130–134. 1992. View Article : Google Scholar : PubMed/NCBI

22 

Simpson DA, Feeney S, Boyle C and Stitt AW: Retinal VEGF mRNA measured by SYBR green I fluorescence: A versatile approach to quantitative PCR. Mol Vis. 6:178–183. 2000.PubMed/NCBI

23 

Pryor RJ and Wittwer CT: Real-time polymerase chain reaction and melting curve analysis. Methods Mol Biol. 336:19–32. 2006.PubMed/NCBI

24 

Kozawa O, Hatakeyama D and Uematsu T: Divergent regulation by p44/p42 MAPK and p38 MAPK of bone morphogenetic protein-4-stimulated osteocalcin synthesis in osteoblasts. J Cell Biochem. 84:583–589. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Kozawa O, Matsuno H and Uematsu T: Involvement of p70 S6 kinase in bone morphogenetic protein signaling: Vascular endothelial growth factor synthesis by bone morphogenetic protein-4 in osteoblasts. J Cell Biochem. 81:430–436. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Tokuda H, Hatakeyama D, Shibata T, Akamatsu S, Oiso Y and Kozawa O: P38 MAPK regulates BMP-4-stimulated VEGF synthesis via p70 S6 kinase in osteoblasts. Am J Physiol Endocrinol Metab. 284:E1202–E1209. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

28 

Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress-induced synthesis of hsp27 and alpha B-crystallin by cyclic AMP in C6 glioma cells. J Neurochem. 66:946–950. 1996. View Article : Google Scholar : PubMed/NCBI

29 

Li Y, Corradetti MN, Inoki K and Guan KL: TSC2: Filling the GAP in the mTOR signaling pathway. Trend Biochem Sci. 29:32–38. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Minamitani C, Tokuda H, Adachi S, Matsushima-Nishiwaki R, Yamauchi J, Kato K, Natsume H, Mizutani J, Kozawa O and Otsuka T: P70 S6 kinase limits tumor necrosis factor-alpha-induced interleukin-6 synthesis in osteoblast-like cells. Mol Cell Endocrinol. 315:195–200. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Ullmann U, Haller J, Decourt JP, Girault N, Girault J, Richard-Caudron AS, Pinceau B and Weber P: A single ascending dose study of epigalocatechin gallate in healthy volunteers. J Int Med Res. 31:88–101. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fujita K, Otsuka T, Yamamoto N, Kainuma S, Ohguchi R, Kawabata T, Sakai G, Kuroyanagi G, Matsushima‑Nishiwaki R, Kozawa O, Kozawa O, et al: (-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts. Exp Ther Med 14: 417-423, 2017.
APA
Fujita, K., Otsuka, T., Yamamoto, N., Kainuma, S., Ohguchi, R., Kawabata, T. ... Tokuda, H. (2017). (-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts. Experimental and Therapeutic Medicine, 14, 417-423. https://doi.org/10.3892/etm.2017.4491
MLA
Fujita, K., Otsuka, T., Yamamoto, N., Kainuma, S., Ohguchi, R., Kawabata, T., Sakai, G., Kuroyanagi, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts". Experimental and Therapeutic Medicine 14.1 (2017): 417-423.
Chicago
Fujita, K., Otsuka, T., Yamamoto, N., Kainuma, S., Ohguchi, R., Kawabata, T., Sakai, G., Kuroyanagi, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts". Experimental and Therapeutic Medicine 14, no. 1 (2017): 417-423. https://doi.org/10.3892/etm.2017.4491
Copy and paste a formatted citation
x
Spandidos Publications style
Fujita K, Otsuka T, Yamamoto N, Kainuma S, Ohguchi R, Kawabata T, Sakai G, Kuroyanagi G, Matsushima‑Nishiwaki R, Kozawa O, Kozawa O, et al: (-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts. Exp Ther Med 14: 417-423, 2017.
APA
Fujita, K., Otsuka, T., Yamamoto, N., Kainuma, S., Ohguchi, R., Kawabata, T. ... Tokuda, H. (2017). (-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts. Experimental and Therapeutic Medicine, 14, 417-423. https://doi.org/10.3892/etm.2017.4491
MLA
Fujita, K., Otsuka, T., Yamamoto, N., Kainuma, S., Ohguchi, R., Kawabata, T., Sakai, G., Kuroyanagi, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts". Experimental and Therapeutic Medicine 14.1 (2017): 417-423.
Chicago
Fujita, K., Otsuka, T., Yamamoto, N., Kainuma, S., Ohguchi, R., Kawabata, T., Sakai, G., Kuroyanagi, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts". Experimental and Therapeutic Medicine 14, no. 1 (2017): 417-423. https://doi.org/10.3892/etm.2017.4491
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