Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer

Wu,Fei; Chen,Jun; Fan,Li‑Mei; Liu,Kai; Zhang,Ning; Li,Shi‑Wen; Zhu,He; Gao,Hai‑Cheng

doi:10.3892/etm.2017.4494

July-2017 Volume 14 Issue 1

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July-2017 Volume 14 Issue 1

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Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer

Authors:
- Fei Wu
- Jun Chen
- Li‑Mei Fan
- Kai Liu
- Ning Zhang
- Shi‑Wen Li
- He Zhu
- Hai‑Cheng Gao
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Affiliations: Department of Gynaecology and Obstetrics, Second Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Clinical Pharmacy, Jilin University School of Pharmaceutical Sciences, Changchun, Jilin 130021, P.R. China

Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 127-130
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Published online on: May 22, 2017

https://doi.org/10.3892/etm.2017.4494
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Abstract

The aim of the present study was to investigate the effect of rutin treatment on the expression of glycogen synthase kinase (GSK)-3β and tumor necrosis factor (TNF)‑α in A549 human lung carcinoma cells. The A549 cells were divided into control, cisplatin and rutin (low, middle and high) groups. ELISA and western blot analysis of TNF‑α expression, 4',6-diamino-2‑phenylindole (DAPI) staining and GSK‑3β immunofluorescence staining were used to investigate the effect of rutin in the human lung carcinoma cells, using cisplatin as a positive control. TNF‑α expression was significantly higher in the rutin and cisplatin groups compared with the control group. Additionally, DAPI staining revealed that the number of apoptotic cells was higher in the rutin and cisplatin groups compared with the control group, and immunofluorescence showed that the expression of GSK‑3β in the cisplatin and rutin groups was significantly higher compared with that in the control group. The results of the present study suggest that rutin promotes the TNF‑α‑induced apoptosis of A549 human lung carcinoma cells. Furthermore, rutin may be able to regulate the expression of GSK‑3β protein in these cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer

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