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Correlation analysis of human embryo LeY glycan antigen expression and embryo quality

  • Authors:
    • Juan Gu
    • Linlin Sui
    • Yanni Ma
    • Zhenzhen Guo
    • Man Zhang
    • Chenyang Zhu
    • Zhu Cai
    • Ying Kong
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, Liaoning 116027, P.R. China
    Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 159-162
    |
    Published online on: May 22, 2017
       https://doi.org/10.3892/etm.2017.4495
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Abstract

This study assessed the feasibility of using LeY glycan secretion level in human embryos as a method of judging embryo quality. Embryo culture media from patients receiving in vitro fertilization-embryo transfer was collected, and quality scores of embryos were recorded. Secretions of LeY in the culture media in different development stages (from 4-cell to 10-cell), embryos in the same development stage of the same patients (8-cell/I) and embryos in the same development stage of different patients (8-cell/I) were examined by dot-blot. Embryos were divided into a hypersecretion group and hyposecretion group, based on their LeY secretion level. The embryo quality was evaluated by clinical observations, the number which developed to D3 cell stage and the number of successful embryo transplantations. LeY secretion increased as embryos developed from 4-cell to 10-cell (P<0.05); secretion of LeY of 8/I is not identical; development speed of embryos with different secretion level of LeY was also different. The number of embryos which developed to 6-cell or higher was 82.2% in the LeY hypersecretion group but only 60% in the hyposecretion group. The rate of successful transplantation was significantly higher in the hypersecretion group (71.1 vs. 40%). In conclusion, LeY glycan secretion level in human embryos is closely related to embryo quality. LeY may become a useful measure to evaluate embryo quality in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Gu J, Sui L, Ma Y, Guo Z, Zhang M, Zhu C, Cai Z and Kong Y: Correlation analysis of human embryo LeY glycan antigen expression and embryo quality. Exp Ther Med 14: 159-162, 2017.
APA
Gu, J., Sui, L., Ma, Y., Guo, Z., Zhang, M., Zhu, C. ... Kong, Y. (2017). Correlation analysis of human embryo LeY glycan antigen expression and embryo quality. Experimental and Therapeutic Medicine, 14, 159-162. https://doi.org/10.3892/etm.2017.4495
MLA
Gu, J., Sui, L., Ma, Y., Guo, Z., Zhang, M., Zhu, C., Cai, Z., Kong, Y."Correlation analysis of human embryo LeY glycan antigen expression and embryo quality". Experimental and Therapeutic Medicine 14.1 (2017): 159-162.
Chicago
Gu, J., Sui, L., Ma, Y., Guo, Z., Zhang, M., Zhu, C., Cai, Z., Kong, Y."Correlation analysis of human embryo LeY glycan antigen expression and embryo quality". Experimental and Therapeutic Medicine 14, no. 1 (2017): 159-162. https://doi.org/10.3892/etm.2017.4495
Copy and paste a formatted citation
x
Spandidos Publications style
Gu J, Sui L, Ma Y, Guo Z, Zhang M, Zhu C, Cai Z and Kong Y: Correlation analysis of human embryo LeY glycan antigen expression and embryo quality. Exp Ther Med 14: 159-162, 2017.
APA
Gu, J., Sui, L., Ma, Y., Guo, Z., Zhang, M., Zhu, C. ... Kong, Y. (2017). Correlation analysis of human embryo LeY glycan antigen expression and embryo quality. Experimental and Therapeutic Medicine, 14, 159-162. https://doi.org/10.3892/etm.2017.4495
MLA
Gu, J., Sui, L., Ma, Y., Guo, Z., Zhang, M., Zhu, C., Cai, Z., Kong, Y."Correlation analysis of human embryo LeY glycan antigen expression and embryo quality". Experimental and Therapeutic Medicine 14.1 (2017): 159-162.
Chicago
Gu, J., Sui, L., Ma, Y., Guo, Z., Zhang, M., Zhu, C., Cai, Z., Kong, Y."Correlation analysis of human embryo LeY glycan antigen expression and embryo quality". Experimental and Therapeutic Medicine 14, no. 1 (2017): 159-162. https://doi.org/10.3892/etm.2017.4495
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