Open Access

Terlipressin protects intestinal epithelial cells against oxygen-glucose deprivation/re-oxygenation injury via the phosphatidylinositol 3-kinase pathway

  • Authors:
    • Zi‑Meng Liu
    • Xu‑Yu Zhang
    • Juan Chen
    • Jian‑Tong Shen
    • Zhi‑Yi Jiang
    • Xiang‑Dong Guan
  • View Affiliations

  • Published online on: May 23, 2017     https://doi.org/10.3892/etm.2017.4502
  • Pages: 260-266
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Intestinal ischemia/reperfusion (I/R) injury is associated with a high morbidity and mortality. Vasopressin is administered to critically ill patients with potential intestinal I/R. However, the impacts of vasopressin on intestinal epithelia under ischemic/anoxic conditions remain unclear. The aim of the present study was to evaluate the effects of terlipressin, a highly selective vasopressin V1 receptor agonist, on oxygen and glucose deprivation/re-oxygenation (OGD/R)‑induced damage in intestinal epithelial cells (IEC‑6). IEC‑6 cells were subjected to OGD for 4 h, followed by 4 h re‑oxygenation. Terlipressin was incubated with cells for 4 h following OGD. Following OGD/R, IEC‑6 cell viability, proliferation and apoptosis, as well as cell cycle dynamics, were assessed and the levels of tumor necrosis factor (TNF)‑α and 15‑F2t‑isoprostane in the culture medium were measured. In addition, wortmannin, a specific phosphatidylinositol 3‑kinase (PI3K) inhibitor, was administrated to investigate the mechanism of terlipressin action. The results demonstrated that IEC‑6 cell viability and proliferation decreased, and cell apoptosis increased, following OGD/R. However, IEC‑6 cell cycle dynamics did not significantly change 4 h after OGD. Incubation with 25 nM terlipressin significantly improved cell viability, proliferation and apoptosis. Furthermore, terlipressin inhibited the secretion of TNF‑α and 15‑F2t‑isoprostane from IEC‑6 cells following OGD/R. The aforementioned effects of terlipressin were completely abolished following the application of 2 µM wortmannin. Therefore, the current study demonstrated that terlipressin administration following OGD attenuates OGD/R‑induced cell damage via the PI3K signaling pathway. These results may help physicians to better understand and more effectively use terlipressin in a clinical setting.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu ZM, Zhang XY, Chen J, Shen JT, Jiang ZY and Guan XD: Terlipressin protects intestinal epithelial cells against oxygen-glucose deprivation/re-oxygenation injury via the phosphatidylinositol 3-kinase pathway. Exp Ther Med 14: 260-266, 2017
APA
Liu, Z., Zhang, X., Chen, J., Shen, J., Jiang, Z., & Guan, X. (2017). Terlipressin protects intestinal epithelial cells against oxygen-glucose deprivation/re-oxygenation injury via the phosphatidylinositol 3-kinase pathway. Experimental and Therapeutic Medicine, 14, 260-266. https://doi.org/10.3892/etm.2017.4502
MLA
Liu, Z., Zhang, X., Chen, J., Shen, J., Jiang, Z., Guan, X."Terlipressin protects intestinal epithelial cells against oxygen-glucose deprivation/re-oxygenation injury via the phosphatidylinositol 3-kinase pathway". Experimental and Therapeutic Medicine 14.1 (2017): 260-266.
Chicago
Liu, Z., Zhang, X., Chen, J., Shen, J., Jiang, Z., Guan, X."Terlipressin protects intestinal epithelial cells against oxygen-glucose deprivation/re-oxygenation injury via the phosphatidylinositol 3-kinase pathway". Experimental and Therapeutic Medicine 14, no. 1 (2017): 260-266. https://doi.org/10.3892/etm.2017.4502