Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection

  • Authors:
    • Yue Yao
    • Yujia Yang
    • Xuehua He
    • Xia Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 495-498
    |
    Published online on: May 25, 2017
       https://doi.org/10.3892/etm.2017.4513
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The upregulation of miR-16-1 expression and heat shock protein 70 (HSP70) and inflammatory reaction mechanism in astrocytes of mice with epilepsy induced by encephalitis B virus infection were studied. Six-to-eight‑week-old healthy male C57BL/6 mice received intraperitoneal injection of pilocarpine (320‑340 mg/kg, 40 mg/ml) to induce status epilepsy. After 7 days, mice were inoculated with 100 µl Dulbecco's modified Eagle's medium (DMEM) in the neck, including 6.25x23 PFU Japanese encephalitis virus P3 wild strain. The experiment was divided into 4 groups, including, the healthy control group, the epilepsy model group, the model group + negative inoculation group and the virus infection group with 10 mice in each group. The healthy control group received intraperitoneal injection of the same amount of normal saline; the model group + negative inoculation group was injected with the same amount of DMEM without P3. One and three days after infection, 5 mice from each group were sacrificed, hippocampus tissues were obtained and astrocytes were isolated. After purification, glial fibrillary acidic protein was identified by immunohistochemical staining. Infected glial cells were detected by P3 antigen of immunofluorescence staining. RT-PCR method was used to detect the expression of miR-16-1 mRNA in astrocytes. Western blot analysis was used to detect the expression of HSP70. ELISA method was used to detect the levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α and nuclear factor-κB (NF-κB) inflammatory factors in tail vein blood. Level of expression of miR-16-1 mRNA, HSP70 as well as IL-6, TNF-α and NF-κB inflammatory factor levels of virus infected mice of 1 and 3 days were significantly higher (P<0.05) than those of model group and negative inoculation group and lowest in control group. In conclusion, the level of expression of miR-16-1 and HSP70 can be increased by the infection of Japanese encephalitis virus on the astrocytes of mice with epilepsy, to promote the expression of IL-6, TNF-α and NF-κB of inflammatory factors.
View Figures
View References

1 

Mirás Veiga A, Moreno DC, Menéndez AI, Siscart IM, Fernández MD, Sánchez EG, González MG and Sáez FG: Effectiveness of electroconvulsive therapy for refractory status epilepticus in febrile infection-related epilepsy syndrome. Neuropediatrics. Jul 19–2016.(Epub ahead of print).

2 

Misra UK, Tan CT and Kalita J: Viral encephalitis and epilepsy. Epilepsia. 49:13–18. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Kielbinski M, Gzielo-Jurek K and Soltys Z: Roles for astrocytes in epilepsy: insights from malformations of cortical development. Neuropathol Appl Neurobiol. 3:1123–1124. 2016.

4 

Li Y, Wang J, Jiang C, Zheng G, Lu X and Guo H: Association of the genetic polymorphisms in pre-microRNAs with risk of childhood epilepsy in a Chinese population. Seizure. 40:21–26. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Kandratavicius L, Hallak JE, Carlotti CG Jr, Assirati JA Jr and Leite JP: Hippocampal expression of heat shock proteins in mesial temporal lobe epilepsy with psychiatric comorbidities and their relation to seizure outcome. Epilepsia. 55:1834–1843. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Hubbard JA, Szu JI, Yonan JM and Binder DK: Regulation of astrocyte glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4) expression in a model of epilepsy. Exp Neurol. 283:85–96. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Robel S: Astroglial scarring and seizures: a cell biological perspective on epilepsy. Neuroscientist. Apr 26–2016.(Epub ahead of print). PubMed/NCBI

8 

Yang J, He F, Meng Q, Sun Y, Wang W and Wang C: Inhibiting HIF-1α decreases expression of TNF-α and caspase-3 in specific brain regions exposed kainic acid-induced status epilepticus. Cell Physiol Biochem. 38:75–82. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Yunoki M, Kurosu T, Koketsu RK, Takahashi K, Okuno Y and Ikuta K: Neutralizing activities of human immunoglobulin derived from donors in Japan against mosquito-borne flaviviruses, Japanese encephalitis virus, West Nile virus, and dengue virus. Biologics. 10:99–102. 2016.PubMed/NCBI

10 

Takamatsu Y, Uchida L and Morita K: Delayed IFN response differentiates replication of West Nile virus and Japanese encephalitis virus in human neuroblastoma and glioblastoma cells. J Gen Virol. 96:2194–2199. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Shen SC, Shen CI, Lin H, Chen CJ, Chang CY, Chen SM, Lee HC, Lai PS and Su HL: Susceptibility of human embryonic stem cell-derived neural cells to Japanese encephalitis virus infection. PLoS One. 9:e1149902014. View Article : Google Scholar : PubMed/NCBI

12 

Azimi A, Hagh MF, Yousefi B, Rahnama MA, Khorrami A, Heydarabad MZ, Najafpour M, Hallajzadeh J and Ghahremani A: The effect of prednisolone on miR-15a and miR-16-1 expression levels and apoptosis in acute lymphoblastic leukemia cell line: CCRF-CEM. Drug Res (Stuttg). 66:432–435. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Reschke CR and Henshall DC: microRNA and epilepsy. Adv Exp Med Biol. 888:41–70. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Alsharafi WA, Xiao B, Abuhamed MM and Luo Z: miRNAs: biological and clinical determinants in epilepsy. Front Mol Neurosci. 8:592015. View Article : Google Scholar : PubMed/NCBI

15 

Srivastava A, Dixit AB, Banerjee J, Tripathi M and Chandra P Sarat: Role of inflammation and its miRNA based regulation in epilepsy: implications for therapy. Clin Chim Acta. 452:1–9. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Goswami S and Banerjee A, Kumari B, Bandopadhyay B, Bhattacharya N, Basu N, Vrati S and Banerjee A: Differential expression and significance of circulating microRNAs in cerebrospinal fluid of acute encephalitis patients infected with Japanese encephalitis virus. Mol Neurobiol. 9:1567–1568. 2016.

17 

Rispoli A, Cipollini E, Catania S, Di Giaimo R, Pulice G, van Houte S, Sparla F, Dal Piaz F, Roncarati D, Trost P, et al: Insights in progressive myoclonus epilepsy: HSP70 promotes cystatin B polymerization. Biochim Biophys Acta. 1834:2591–2599. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Rejdak K, Kuhle J, Rüegg S, Lindberg RL, Petzold A, Sulejczak D, Papuc E, Rejdak R, Stelmasiak Z and Grieb P: Neurofilament heavy chain and heat shock protein 70 as markers of seizure-related brain injury. Epilepsia. 53:922–927. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Turturici G, Sconzo G and Geraci F: Hsp70 and its molecular role in nervous system diseases. Biochem Res Int. 2011:6181272011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Yang Y, He X and Wang X: miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection. Exp Ther Med 14: 495-498, 2017.
APA
Yao, Y., Yang, Y., He, X., & Wang, X. (2017). miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection. Experimental and Therapeutic Medicine, 14, 495-498. https://doi.org/10.3892/etm.2017.4513
MLA
Yao, Y., Yang, Y., He, X., Wang, X."miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection". Experimental and Therapeutic Medicine 14.1 (2017): 495-498.
Chicago
Yao, Y., Yang, Y., He, X., Wang, X."miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection". Experimental and Therapeutic Medicine 14, no. 1 (2017): 495-498. https://doi.org/10.3892/etm.2017.4513
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Yang Y, He X and Wang X: miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection. Exp Ther Med 14: 495-498, 2017.
APA
Yao, Y., Yang, Y., He, X., & Wang, X. (2017). miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection. Experimental and Therapeutic Medicine, 14, 495-498. https://doi.org/10.3892/etm.2017.4513
MLA
Yao, Y., Yang, Y., He, X., Wang, X."miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection". Experimental and Therapeutic Medicine 14.1 (2017): 495-498.
Chicago
Yao, Y., Yang, Y., He, X., Wang, X."miR-16-1 expression, heat shock protein 70 and inflammatory reactions in astrocytes of mice with epilepsy induced by encephalitis B virus infection". Experimental and Therapeutic Medicine 14, no. 1 (2017): 495-498. https://doi.org/10.3892/etm.2017.4513
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team