The effects and possible mechanism of β2AR gene expression in cardiocytes of canines with heart failure

Gong,Haibin; San,Yu; Wang,Lei; Lv,Qian; Chen,Libin

doi:10.3892/etm.2017.4521

July-2017 Volume 14 Issue 1

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July-2017 Volume 14 Issue 1

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Article Open Access

The effects and possible mechanism of β2AR gene expression in cardiocytes of canines with heart failure

Authors:
- Haibin Gong
- Yu San
- Lei Wang
- Qian Lv
- Libin Chen
View Affiliations / Copyright

Affiliations: Department of Cardiology, Xuzhou Central Hospital, Xuzhou Cardiovascular Disease Institute, Xuzhou, Jiangsu 221009, P.R. China

Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 539-546
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Published online on: May 30, 2017

https://doi.org/10.3892/etm.2017.4521
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Abstract

The objective of the present study was to observe the changes of β2‑adrenergic receptor (β2AR) protein expression in a canine model of heart failure (HF), and the function of cardiocytes after transfection with Adv‑β2AR. The canine model of chronic HF was induced by rapid right ventricular pacing and cardiocytes were isolated with collagenase II. Cardiocytes were transfected with Adv‑β2AR to observe contractile function with a motion edge‑detection system of single cells. Expression of β2AR protein in cardiocytes was measured by immunoblotting and the levels of intracellular cAMP were measured by ELISA. Compared with the control group (the sham group), the expression of β2AR protein in HF cardiocytes did not change, but the basal (1 mM Ca2+) contraction amplitude percentage (1.809±0.922 vs. 1.120±0.432%, P<0.05), the maximum contraction amplitude percentage (14.855±2.377 vs. 10.784±2.675%, P<0.01) and the basal levels of intracellular cAMP (9.39±2.54 vs. 5.26±0.95 pmol/ml, n=6, P<0.05) of HF cardiocytes were significantly decreased. However, when HF cardiocytes were transfected with Adv‑β2AR and cultured for 48 h, compared with the non‑transfected group, the basal contraction amplitude percentage (0.851±0.324 vs. 1.629±0.522%, P<0.05), the maximum contraction amplitude percentage (9.260±2.208% vs. 12.205±1.437%, P<0.01) and the basal levels of intracellular cAMP (5.26±0.95 vs. 9.03±1.03 pmol/ml, n=6, P<0.05) of cardiocytes in the transfected group were significantly increased. In conclusion, the expression of β2AR protein in HF cardiocytes did not change, but contraction function was impaired. The moderate overexpression of β2AR gene in the HF cardiocytes increased the levels of intracellular cAMP and improved contraction function.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The effects and possible mechanism of β2AR gene expression in cardiocytes of canines with heart failure

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