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JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells

  • Authors:
    • Yaowu Zhang
    • Pingbo Chen
    • Hangang Hong
    • Lei Wang
    • Yubo Zhou
    • Yi Lang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 593-599
    |
    Published online on: May 31, 2017
       https://doi.org/10.3892/etm.2017.4529
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Abstract

Human osteosarcoma is a common primary malignancy of the bone in children and adolescents. It has been reported that curcumin is able to induce apoptosis in osteosarcoma MG63 cells through the mitochondrial pathway. However, whether curcumin is able to induce autophagy and the interaction between apoptosis and autophagy in osteosarcoma cells has yet to be fully elucidated. In the current study, it was determined that curcumin was able to significantly induce apoptosis, and lead to autophagy in MG63 cells. Notably, inhibition of apoptosis enhanced curcumin‑induced autophagy due to upregulation of the c‑Jun N‑terminal kinase (JNK) signaling pathway. This finding was confirmed by the use of JNK‑specific inhibitor, SP600125. Furthermore, our data showed that curcumin‑induced apoptosis was increased when autophagy was completely inhibited by 3‑methyladenine in MG63 cells. These results suggest that autophagy may have an important role in resistance to apoptosis when MG63 cells are incubated with curcumin. Thus, these results provide important insights into the interaction between apoptosis and autophagy in osteosarcoma cells and clinical treatment strategies using curcumin.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Chen P, Hong H, Wang L, Zhou Y and Lang Y: JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells. Exp Ther Med 14: 593-599, 2017.
APA
Zhang, Y., Chen, P., Hong, H., Wang, L., Zhou, Y., & Lang, Y. (2017). JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells. Experimental and Therapeutic Medicine, 14, 593-599. https://doi.org/10.3892/etm.2017.4529
MLA
Zhang, Y., Chen, P., Hong, H., Wang, L., Zhou, Y., Lang, Y."JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells". Experimental and Therapeutic Medicine 14.1 (2017): 593-599.
Chicago
Zhang, Y., Chen, P., Hong, H., Wang, L., Zhou, Y., Lang, Y."JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells". Experimental and Therapeutic Medicine 14, no. 1 (2017): 593-599. https://doi.org/10.3892/etm.2017.4529
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Chen P, Hong H, Wang L, Zhou Y and Lang Y: JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells. Exp Ther Med 14: 593-599, 2017.
APA
Zhang, Y., Chen, P., Hong, H., Wang, L., Zhou, Y., & Lang, Y. (2017). JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells. Experimental and Therapeutic Medicine, 14, 593-599. https://doi.org/10.3892/etm.2017.4529
MLA
Zhang, Y., Chen, P., Hong, H., Wang, L., Zhou, Y., Lang, Y."JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells". Experimental and Therapeutic Medicine 14.1 (2017): 593-599.
Chicago
Zhang, Y., Chen, P., Hong, H., Wang, L., Zhou, Y., Lang, Y."JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells". Experimental and Therapeutic Medicine 14, no. 1 (2017): 593-599. https://doi.org/10.3892/etm.2017.4529
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