Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells

  • Authors:
    • You‑Ping Deng
    • Ying‑Juan Liu
    • Zhan‑Qiu Yang
    • Yan‑Jun Wang
    • Bing‑Yan He
    • Pin Liu
  • View Affiliations

  • Published online on: June 1, 2017     https://doi.org/10.3892/etm.2017.4533
  • Pages: 753-758
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Abstract

Human bocavirus (HBoV) is classified in the Bocavirus genus within the Parvoviridae family, first identified from children with respiratory diseases. Previous studies have investigated the stimulating effect of HBoV on cell apoptosis and autophagy. In the present study, human bronchial epithelial cells (HBECs) were utilized to examine the mechanism of HBoV recombination expressing vector (pWHL‑1) on the promotion of cell apoptosis and autophagy. The results from the present study indicated that pWHL‑1 inhibited the proliferation of HBECs in a time‑dependent manner. Additionally, pWHL‑1induced apoptosis, as substantiated by an increased apoptotic rate and presence of autophagosomes. Following pWHL‑1 transfection, proliferating cell nuclear antigen, caspase‑3 and B cell lymphoma 2 (Bcl‑2) protein expression levels were decreased, with the exception of Bcl‑2 associated x (Bax) protein, which increased. mRNA and protein expression levels of microtubule‑associated protein 1A/1B‑light chain 3 (LC3) II and autophagy protein 5 were increased in pWHL‑1‑transfected HBECs, whereas, the mRNA and protein levels of LC3I and sequestosome 1 were decreased. Notably, pWHL‑1 also enhanced the activation of p53 and inhibited AKT activation in HBECs. Results from the present study suggest that pWHL‑1 induces apoptosis and autophagy, thus providing a novel insight into the effect of HBoV and its uses in respiratory diseases.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Deng YP, Liu YJ, Yang ZQ, Wang YJ, He BY and Liu P: Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells. Exp Ther Med 14: 753-758, 2017
APA
Deng, Y., Liu, Y., Yang, Z., Wang, Y., He, B., & Liu, P. (2017). Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells. Experimental and Therapeutic Medicine, 14, 753-758. https://doi.org/10.3892/etm.2017.4533
MLA
Deng, Y., Liu, Y., Yang, Z., Wang, Y., He, B., Liu, P."Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells". Experimental and Therapeutic Medicine 14.1 (2017): 753-758.
Chicago
Deng, Y., Liu, Y., Yang, Z., Wang, Y., He, B., Liu, P."Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells". Experimental and Therapeutic Medicine 14, no. 1 (2017): 753-758. https://doi.org/10.3892/etm.2017.4533