Open Access

Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model

  • Authors:
    • Jiannan Li
    • Jianying Zeng
    • Jibing Chen
    • Jian Shi
    • Xiaomei Luo
    • Gang Fang
    • Wei Chai
    • Wenlong Zhang
    • Tongjun Liu
    • Lizhi Niu
  • View Affiliations

  • Published online on: June 7, 2017     https://doi.org/10.3892/etm.2017.4559
  • Pages: 696-702
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to evaluate the effects of irreversible electroporation (IRE) on the stomach wall following the direct application of IRE onto the organ surface. IRE ablation was performed in 8 Tibetan mini‑pigs, which were randomly assigned into two groups based on their ablated areas: Group A, gastric cardia, fundus of stomach, gastric body and group B, lesser gastric curvature, greater gastric curvature, stomach pylorus. Two IRE needles were placed in the space between the stomach wall and the liver (not inserted into the stomach tissue), and three lesions were created in each pig. Serum aminotransferase and white blood cell (WBC) levels were measured. Gastroscopy and endoscopic ultrasonography were performed. From each group, 2 pigs were sacrificed on day 7 post‑IRE; the remaining pigs were sacrificed on day 28 post‑IRE. There were no signs of perforation on the stomach wall. Serum aminotransferase and WBC levels increased in both groups on day 1 post‑IRE and decreased gradually thereafter. The gastroscopy procedure revealed oval ulcers on day 7 post‑IRE and smaller ulcers on day 28 post‑IRE. Transmural necrosis, inflammation and fibrosis were observed at 7 days post‑IRE. Healing ulcers were observed at 28 days post‑IRE. In conclusion, IRE ablation caused damage to the stomach wall; however, IRE did not induce any perforation.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li J, Zeng J, Chen J, Shi J, Luo X, Fang G, Chai W, Zhang W, Liu T, Niu L, Niu L, et al: Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model. Exp Ther Med 14: 696-702, 2017
APA
Li, J., Zeng, J., Chen, J., Shi, J., Luo, X., Fang, G. ... Niu, L. (2017). Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model. Experimental and Therapeutic Medicine, 14, 696-702. https://doi.org/10.3892/etm.2017.4559
MLA
Li, J., Zeng, J., Chen, J., Shi, J., Luo, X., Fang, G., Chai, W., Zhang, W., Liu, T., Niu, L."Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model". Experimental and Therapeutic Medicine 14.1 (2017): 696-702.
Chicago
Li, J., Zeng, J., Chen, J., Shi, J., Luo, X., Fang, G., Chai, W., Zhang, W., Liu, T., Niu, L."Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model". Experimental and Therapeutic Medicine 14, no. 1 (2017): 696-702. https://doi.org/10.3892/etm.2017.4559