Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model

Li,Jiannan; Zeng,Jianying; Chen,Jibing; Shi,Jian; Luo,Xiaomei; Fang,Gang; Chai,Wei; Zhang,Wenlong; Liu,Tongjun; Niu,Lizhi

doi:10.3892/etm.2017.4559

July-2017 Volume 14 Issue 1

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July-2017 Volume 14 Issue 1

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Article Open Access

Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model

Authors:
- Jiannan Li
- Jianying Zeng
- Jibing Chen
- Jian Shi
- Xiaomei Luo
- Gang Fang
- Wei Chai
- Wenlong Zhang
- Tongjun Liu
- Lizhi Niu
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Affiliations: Department of General Surgery, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Central Laboratory, Fuda Cancer Hospital, Jinan University School of Medicine, Guangzhou, Guangdong 510665, P.R. China, Department of Gynecology and Obstetrics, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Hematology and Oncology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130031, P.R. China

Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 696-702
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Published online on: June 7, 2017

https://doi.org/10.3892/etm.2017.4559
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Abstract

The aim of the present study was to evaluate the effects of irreversible electroporation (IRE) on the stomach wall following the direct application of IRE onto the organ surface. IRE ablation was performed in 8 Tibetan mini‑pigs, which were randomly assigned into two groups based on their ablated areas: Group A, gastric cardia, fundus of stomach, gastric body and group B, lesser gastric curvature, greater gastric curvature, stomach pylorus. Two IRE needles were placed in the space between the stomach wall and the liver (not inserted into the stomach tissue), and three lesions were created in each pig. Serum aminotransferase and white blood cell (WBC) levels were measured. Gastroscopy and endoscopic ultrasonography were performed. From each group, 2 pigs were sacrificed on day 7 post‑IRE; the remaining pigs were sacrificed on day 28 post‑IRE. There were no signs of perforation on the stomach wall. Serum aminotransferase and WBC levels increased in both groups on day 1 post‑IRE and decreased gradually thereafter. The gastroscopy procedure revealed oval ulcers on day 7 post‑IRE and smaller ulcers on day 28 post‑IRE. Transmural necrosis, inflammation and fibrosis were observed at 7 days post‑IRE. Healing ulcers were observed at 28 days post‑IRE. In conclusion, IRE ablation caused damage to the stomach wall; however, IRE did not induce any perforation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model

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