Open Access

Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly

  • Authors:
    • Fan Jiang
    • Ning Liu
    • Xiao Zhuang Chen
    • Kun Yuan Han
    • Cai Zhong Zhu
  • View Affiliations

  • Published online on: June 30, 2017     https://doi.org/10.3892/etm.2017.4714
  • Pages: 2031-2035
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to examine the correlation between KCNJ11 gene polymorphism and metabolic syndrome in elderly patients. From January 2014 to January 2015, 54 elderly patients with metabolic syndrome were enrolled in this study as the observation group. During the same period, 46 healthy elderly individuals were enrolled in this study as the control group. KCNJ11 gene polymorphism (rs28502) was analyzed using polymerase chain reaction-restriction fragment length polymorphism. The expression levels of mRNA in different genotypes were detected using FQ-PCR. ELISA was used to evaluate the KCNJ11 protein expression in different genotypes. KCNJ11 gene polymorphism and metabolic syndrome was studied by measuring the blood pressure levels in patients with different genotypes. Three genotypes of KCNJ11 gene in rs28502 were CC, CT and TT. The CC, CT and TT genotype frequencies in healthy population were 8.5, 9.2 and 82.2%, respectively, while the genotype frequencies in patients with metabolic syndrome were 42.4, 49.8 and 7.8%, respectively. There were significant differences between groups (P≤0.05). However, the genotype frequencies of C/T in healthy individuals and metabolic syndrome patients were 35.3 and 38.3%, respectively. There were no significant differences between groups (P>0.05). FQ-PCR results showed that the KCNJ11 mRNA expression levels in the control and observation groups had no significant differences (P>0.05). However, the results obtained from ELISA analysis revealed that KCNJ11 protein expression level in the observation group was significantly higher than that in the control group (P<0.05). In conclusion, KCNJ11 gene polymorphism is associated with metabolic syndrome in the elderly. Elderly patients with the CC and TT genotypes are more likely to develop metabolic syndrome.

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September-2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jiang F, Liu N, Chen X, Han K and Zhu C: Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly. Exp Ther Med 14: 2031-2035, 2017
APA
Jiang, F., Liu, N., Chen, X., Han, K., & Zhu, C. (2017). Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly. Experimental and Therapeutic Medicine, 14, 2031-2035. https://doi.org/10.3892/etm.2017.4714
MLA
Jiang, F., Liu, N., Chen, X., Han, K., Zhu, C."Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly". Experimental and Therapeutic Medicine 14.3 (2017): 2031-2035.
Chicago
Jiang, F., Liu, N., Chen, X., Han, K., Zhu, C."Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly". Experimental and Therapeutic Medicine 14, no. 3 (2017): 2031-2035. https://doi.org/10.3892/etm.2017.4714