Evaluation of hyaluronic acid‑P40 conjugated cream in a mouse model of dermatitis induced by oxazolone
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- Published online on: July 19, 2017 https://doi.org/10.3892/etm.2017.4810
- Pages: 2439-2444
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Copyright: © Mangano et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
P40 is a particulate fraction or fragment isolated from Corynebacterium granulosum, which exhibits a wide spectrum of pharmacological functions including antitumor, antibacterial, phagocytic, antiviral and cytokine induction effects. In the present study, the immunomodulatory potential of P40‑conjugated with hyaluronic acid was assessed in a mouse model of dermatitis induced by oxazolone. Oxazolone‑induced allergic contact dermatitis is a T cell‑mediated Th2‑like hypersensitivity reaction, which mimics the corresponding reaction in humans. Female cluster of differentiation‑1 mice were sensitized on days 0 and 1 by the application of 2% oxazolone onto a shaved back. The disease was induced by re‑challenge on day 7 using 15% oxazolone in the inner and outer of the left ears of the mice. Mice were topically treated with hyaluronic acid‑P40 conjugate cream or with placebo to the inner and outer surface of the left ear for 7 consecutive days starting from 1 h after the sensitization. A significant reduction in ear thickness and weight and in edema and leukocyte recruitment were observed in the mice treated with hyaluronic‑P40 conjugate cream compared with mice treated with the cream base alone (P<0.05). Thus, P40‑conjugated with hyaluronic acid may constitute an innovative dermatitis treatment.